The preparation and application of calcium phosphate biomedical composites in filling of weight-bearing bone defects

Abstract Nowadays, artificial bone materials have been widely applied in the filling of non-weight bearing bone defects, but scarcely ever in weight-bearing bone defects. This study aims to develop an artificial bone with excellent mechanical properties and good osteogenic capability. Firstly, the c...

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Autores principales:	Lijia Cheng, Tianchang Lin, Ahmad Taha Khalaf, Yamei Zhang, Hongyan He, Liming Yang, Shuo Yan, Jiang Zhu, Zheng Shi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e39f02315cb4b1c9385c08e49023f17
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spelling	oai:doaj.org-article:7e39f02315cb4b1c9385c08e49023f172021-12-02T10:54:30ZThe preparation and application of calcium phosphate biomedical composites in filling of weight-bearing bone defects10.1038/s41598-021-83941-32045-2322https://doaj.org/article/7e39f02315cb4b1c9385c08e49023f172021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83941-3https://doaj.org/toc/2045-2322Abstract Nowadays, artificial bone materials have been widely applied in the filling of non-weight bearing bone defects, but scarcely ever in weight-bearing bone defects. This study aims to develop an artificial bone with excellent mechanical properties and good osteogenic capability. Firstly, the collagen-thermosensitive hydrogel-calcium phosphate (CTC) composites were prepared as follows: dissolving thermosensitive hydrogel at 4 °C, then mixing with type I collagen as well as tricalcium phosphate (CaP) powder, and moulding the composites at 37 °C. Next, the CTC composites were subjected to evaluate for their chemical composition, micro morphology, pore size, Shore durometer, porosity and water absorption ability. Following this, the CTC composites were implanted into the muscle of mice while the 70% hydroxyapatite/30% β-tricalcium phosphate (HA/TCP) biomaterials were set as the control group; 8 weeks later, the osteoinductive abilities of biomaterials were detected by histological staining. Finally, the CTC and HA/TCP biomaterials were used to fill the large segments of tibia defects in mice. The bone repairing and load-bearing abilities of materials were evaluated by histological staining, X-ray and micro-CT at week 8. Both the CTC and HA/TCP biomaterials could induce ectopic bone formation in mice; however, the CTC composites tended to produce larger areas of bone and bone marrow tissues than HA/TCP. Simultaneously, bone-repairing experiments showed that HA/TCP biomaterials were easily crushed or pushed out by new bone growth as the material has a poor hardness. In comparison, the CTC composites could be replaced gradually by newly formed bone and repair larger segments of bone defects. The CTC composites trialled in this study have better mechanical properties, osteoinductivity and weight-bearing capacity than HA/TCP. The CTC composites provide an experimental foundation for the synthesis of artificial bone and a new option for orthopedic patients.Lijia ChengTianchang LinAhmad Taha KhalafYamei ZhangHongyan HeLiming YangShuo YanJiang ZhuZheng ShiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lijia Cheng Tianchang Lin Ahmad Taha Khalaf Yamei Zhang Hongyan He Liming Yang Shuo Yan Jiang Zhu Zheng Shi The preparation and application of calcium phosphate biomedical composites in filling of weight-bearing bone defects
description	Abstract Nowadays, artificial bone materials have been widely applied in the filling of non-weight bearing bone defects, but scarcely ever in weight-bearing bone defects. This study aims to develop an artificial bone with excellent mechanical properties and good osteogenic capability. Firstly, the collagen-thermosensitive hydrogel-calcium phosphate (CTC) composites were prepared as follows: dissolving thermosensitive hydrogel at 4 °C, then mixing with type I collagen as well as tricalcium phosphate (CaP) powder, and moulding the composites at 37 °C. Next, the CTC composites were subjected to evaluate for their chemical composition, micro morphology, pore size, Shore durometer, porosity and water absorption ability. Following this, the CTC composites were implanted into the muscle of mice while the 70% hydroxyapatite/30% β-tricalcium phosphate (HA/TCP) biomaterials were set as the control group; 8 weeks later, the osteoinductive abilities of biomaterials were detected by histological staining. Finally, the CTC and HA/TCP biomaterials were used to fill the large segments of tibia defects in mice. The bone repairing and load-bearing abilities of materials were evaluated by histological staining, X-ray and micro-CT at week 8. Both the CTC and HA/TCP biomaterials could induce ectopic bone formation in mice; however, the CTC composites tended to produce larger areas of bone and bone marrow tissues than HA/TCP. Simultaneously, bone-repairing experiments showed that HA/TCP biomaterials were easily crushed or pushed out by new bone growth as the material has a poor hardness. In comparison, the CTC composites could be replaced gradually by newly formed bone and repair larger segments of bone defects. The CTC composites trialled in this study have better mechanical properties, osteoinductivity and weight-bearing capacity than HA/TCP. The CTC composites provide an experimental foundation for the synthesis of artificial bone and a new option for orthopedic patients.
format	article
author	Lijia Cheng Tianchang Lin Ahmad Taha Khalaf Yamei Zhang Hongyan He Liming Yang Shuo Yan Jiang Zhu Zheng Shi
author_facet	Lijia Cheng Tianchang Lin Ahmad Taha Khalaf Yamei Zhang Hongyan He Liming Yang Shuo Yan Jiang Zhu Zheng Shi
author_sort	Lijia Cheng
title	The preparation and application of calcium phosphate biomedical composites in filling of weight-bearing bone defects
title_short	The preparation and application of calcium phosphate biomedical composites in filling of weight-bearing bone defects
title_full	The preparation and application of calcium phosphate biomedical composites in filling of weight-bearing bone defects
title_fullStr	The preparation and application of calcium phosphate biomedical composites in filling of weight-bearing bone defects
title_full_unstemmed	The preparation and application of calcium phosphate biomedical composites in filling of weight-bearing bone defects
title_sort	preparation and application of calcium phosphate biomedical composites in filling of weight-bearing bone defects
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7e39f02315cb4b1c9385c08e49023f17
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The preparation and application of calcium phosphate biomedical composites in filling of weight-bearing bone defects

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