Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT

We established a syntrophic coculture of Syntrophobacter fumaroxidans MPOBT (SF) and Geobacter sulfurreducens PCAT (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen,...

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Autores principales:	Monir Mollaei, Maria Suarez-Diez, Vicente T. Sedano-Nunez, Sjef Boeren, Alfons J. M. Stams, Caroline M. Plugge
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Syntrophobacter fumaroxidans Geobacter sulfurreducens coculture interspecies electron transfer propionate proteomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7e3f855e6fa94753918a97a03e84d944
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spelling	oai:doaj.org-article:7e3f855e6fa94753918a97a03e84d9442021-12-01T18:11:33ZProteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT1664-302X10.3389/fmicb.2021.708911https://doaj.org/article/7e3f855e6fa94753918a97a03e84d9442021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.708911/fullhttps://doaj.org/toc/1664-302XWe established a syntrophic coculture of Syntrophobacter fumaroxidans MPOBT (SF) and Geobacter sulfurreducens PCAT (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron acceptor. Proteomic analyses of the SF-GS coculture revealed propionate conversion via the methylmalonyl-CoA (MMC) pathway by SF. The possibility of interspecies electron transfer (IET) via direct (DIET) and/or hydrogen/formate transfer (HFIT) was investigated by comparing the differential abundance of associated proteins in SF-GS coculture against (i) SF coculture with Methanospirillum hungatei (SF-MH), which relies on HFIT, (ii) GS pure culture growing on acetate, formate, hydrogen as propionate products, and Fe(III). We noted some evidence for DIET in the SF-GS coculture, i.e., GS in the coculture showed significantly lower abundance of uptake hydrogenase (43-fold) and formate dehydrogenase (45-fold) and significantly higher abundance of proteins related to acetate metabolism (i.e., GltA; 62-fold) compared to GS pure culture. Moreover, SF in the SF-GS coculture showed significantly lower abundance of IET-related formate dehydrogenases, Fdh3 (51-fold) and Fdh5 (29-fold), and the rate of propionate conversion in SF-GS was 8-fold lower than in the SF-MH coculture. In contrast, compared to GS pure culture, we found lower abundance of pilus-associated cytochrome OmcS (2-fold) and piliA (5-fold) in the SF-GS coculture that is suggested to be necessary for DIET. Furthermore, neither visible aggregates formed in the SF-GS coculture, nor the pili-E of SF (suggested as e-pili) were detected. These findings suggest that the IET mechanism is complex in the SF-GS coculture and can be mediated by several mechanisms rather than one discrete pathway. Our study can be further useful in understanding syntrophic propionate degradation in bioelectrochemical and anaerobic digestion systems.Monir MollaeiMonir MollaeiMaria Suarez-DiezVicente T. Sedano-NunezSjef BoerenAlfons J. M. StamsAlfons J. M. StamsCaroline M. PluggeCaroline M. PluggeFrontiers Media S.A.articleSyntrophobacter fumaroxidansGeobacter sulfurreducenscocultureinterspecies electron transferpropionateproteomicsMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Syntrophobacter fumaroxidans Geobacter sulfurreducens coculture interspecies electron transfer propionate proteomics Microbiology QR1-502
spellingShingle	Syntrophobacter fumaroxidans Geobacter sulfurreducens coculture interspecies electron transfer propionate proteomics Microbiology QR1-502 Monir Mollaei Monir Mollaei Maria Suarez-Diez Vicente T. Sedano-Nunez Sjef Boeren Alfons J. M. Stams Alfons J. M. Stams Caroline M. Plugge Caroline M. Plugge Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
description	We established a syntrophic coculture of Syntrophobacter fumaroxidans MPOBT (SF) and Geobacter sulfurreducens PCAT (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron acceptor. Proteomic analyses of the SF-GS coculture revealed propionate conversion via the methylmalonyl-CoA (MMC) pathway by SF. The possibility of interspecies electron transfer (IET) via direct (DIET) and/or hydrogen/formate transfer (HFIT) was investigated by comparing the differential abundance of associated proteins in SF-GS coculture against (i) SF coculture with Methanospirillum hungatei (SF-MH), which relies on HFIT, (ii) GS pure culture growing on acetate, formate, hydrogen as propionate products, and Fe(III). We noted some evidence for DIET in the SF-GS coculture, i.e., GS in the coculture showed significantly lower abundance of uptake hydrogenase (43-fold) and formate dehydrogenase (45-fold) and significantly higher abundance of proteins related to acetate metabolism (i.e., GltA; 62-fold) compared to GS pure culture. Moreover, SF in the SF-GS coculture showed significantly lower abundance of IET-related formate dehydrogenases, Fdh3 (51-fold) and Fdh5 (29-fold), and the rate of propionate conversion in SF-GS was 8-fold lower than in the SF-MH coculture. In contrast, compared to GS pure culture, we found lower abundance of pilus-associated cytochrome OmcS (2-fold) and piliA (5-fold) in the SF-GS coculture that is suggested to be necessary for DIET. Furthermore, neither visible aggregates formed in the SF-GS coculture, nor the pili-E of SF (suggested as e-pili) were detected. These findings suggest that the IET mechanism is complex in the SF-GS coculture and can be mediated by several mechanisms rather than one discrete pathway. Our study can be further useful in understanding syntrophic propionate degradation in bioelectrochemical and anaerobic digestion systems.
format	article
author	Monir Mollaei Monir Mollaei Maria Suarez-Diez Vicente T. Sedano-Nunez Sjef Boeren Alfons J. M. Stams Alfons J. M. Stams Caroline M. Plugge Caroline M. Plugge
author_facet	Monir Mollaei Monir Mollaei Maria Suarez-Diez Vicente T. Sedano-Nunez Sjef Boeren Alfons J. M. Stams Alfons J. M. Stams Caroline M. Plugge Caroline M. Plugge
author_sort	Monir Mollaei
title	Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
title_short	Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
title_full	Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
title_fullStr	Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
title_full_unstemmed	Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT
title_sort	proteomic analysis of a syntrophic coculture of syntrophobacter fumaroxidans mpobt and geobacter sulfurreducens pcat
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/7e3f855e6fa94753918a97a03e84d944
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Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT

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