Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures

Abstract Cardiac magnetic resonance (CMR) has emerged as a powerful tool for in vivo assessments of cardiac parameters in experimental animal models of cardiovascular diseases, but its reproducibility in this setting remains poorly explored. To address this issue, we investigated the test-retest rep...

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Autores principales:	Michael Joubert, Pia Tager, Damien Legallois, Estelle Defourneaux, Bastien Le Guellec, Bernhard Gerber, Remy Morello, Alain Manrique
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e4a55d4dd284c8f9a36c6af00636860
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spelling	oai:doaj.org-article:7e4a55d4dd284c8f9a36c6af006368602021-12-02T16:06:57ZTest-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures10.1038/s41598-017-07083-12045-2322https://doaj.org/article/7e4a55d4dd284c8f9a36c6af006368602017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07083-1https://doaj.org/toc/2045-2322Abstract Cardiac magnetic resonance (CMR) has emerged as a powerful tool for in vivo assessments of cardiac parameters in experimental animal models of cardiovascular diseases, but its reproducibility in this setting remains poorly explored. To address this issue, we investigated the test-retest reproducibility of preclinical cardiac magnetic resonance imaging (CMR) at 7 Tesla in healthy C57BL/6 mice, including an analysis of the impact of different anesthetic procedures (isoflurane or pentobarbital). We also analyzed the intra-study reproducibility and the intra- and inter-observer post-processing reproducibility of CMR images. Test-retest reproducibility was high for left ventricular parameters, especially with the isoflurane anesthetic procedure, whereas right ventricular parameters and deformation measurements were less reproducible, mainly due to physiological variability. Post-processing reproducibility of CMR images was high both within and between observers. These results highlight that anesthetic procedures might influence CMR test-retest reproducibility, an important ethical consideration for longitudinal studies in rodent models of cardiomyopathy to limit the number of animals used.Michael JoubertPia TagerDamien LegalloisEstelle DefourneauxBastien Le GuellecBernhard GerberRemy MorelloAlain ManriqueNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael Joubert Pia Tager Damien Legallois Estelle Defourneaux Bastien Le Guellec Bernhard Gerber Remy Morello Alain Manrique Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
description	Abstract Cardiac magnetic resonance (CMR) has emerged as a powerful tool for in vivo assessments of cardiac parameters in experimental animal models of cardiovascular diseases, but its reproducibility in this setting remains poorly explored. To address this issue, we investigated the test-retest reproducibility of preclinical cardiac magnetic resonance imaging (CMR) at 7 Tesla in healthy C57BL/6 mice, including an analysis of the impact of different anesthetic procedures (isoflurane or pentobarbital). We also analyzed the intra-study reproducibility and the intra- and inter-observer post-processing reproducibility of CMR images. Test-retest reproducibility was high for left ventricular parameters, especially with the isoflurane anesthetic procedure, whereas right ventricular parameters and deformation measurements were less reproducible, mainly due to physiological variability. Post-processing reproducibility of CMR images was high both within and between observers. These results highlight that anesthetic procedures might influence CMR test-retest reproducibility, an important ethical consideration for longitudinal studies in rodent models of cardiomyopathy to limit the number of animals used.
format	article
author	Michael Joubert Pia Tager Damien Legallois Estelle Defourneaux Bastien Le Guellec Bernhard Gerber Remy Morello Alain Manrique
author_facet	Michael Joubert Pia Tager Damien Legallois Estelle Defourneaux Bastien Le Guellec Bernhard Gerber Remy Morello Alain Manrique
author_sort	Michael Joubert
title	Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
title_short	Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
title_full	Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
title_fullStr	Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
title_full_unstemmed	Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures
title_sort	test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-tesla: effect of anesthetic procedures
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7e4a55d4dd284c8f9a36c6af00636860
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Test-retest reproducibility of cardiac magnetic resonance imaging in healthy mice at 7-Tesla: effect of anesthetic procedures

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