Multi-Mechanistic In Vitro Evaluation of Antihyperglycemic, Antioxidant and Antiglycation Activities of Three Phenolic-Rich Indian Red Rice Genotypes and In Silico Evaluation of Their Phenolic Metabolites

The study evaluated the antidiabetic potential of three traditional Indian red rice genotypes/RR (Kattuyanam/KA, Chennangi/CH & Karungkuruvai/KU) using a combination of in vitro, metabolomics (Quadrupole-Time of Flight-Liquid chromatography-Mass spectrometry/Q-TOF-LC-MS/MS)<i>,</i> a...

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Autores principales:	Ashrita C. Haldipur, Nagarajan Srividya
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antidiabetic AGEs metabolomics phenolic profile molecular docking Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/7e4b3cb68a5a4c76b4a3876159dbc1b6
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spelling	oai:doaj.org-article:7e4b3cb68a5a4c76b4a3876159dbc1b62021-11-25T17:35:59ZMulti-Mechanistic In Vitro Evaluation of Antihyperglycemic, Antioxidant and Antiglycation Activities of Three Phenolic-Rich Indian Red Rice Genotypes and In Silico Evaluation of Their Phenolic Metabolites10.3390/foods101128182304-8158https://doaj.org/article/7e4b3cb68a5a4c76b4a3876159dbc1b62021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2818https://doaj.org/toc/2304-8158The study evaluated the antidiabetic potential of three traditional Indian red rice genotypes/RR (Kattuyanam/KA, Chennangi/CH & Karungkuruvai/KU) using a combination of in vitro, metabolomics (Quadrupole-Time of Flight-Liquid chromatography-Mass spectrometry/Q-TOF-LC-MS/MS)<i>,</i> and in silico techniques. In terms of antihyperglycemic potential, KA exhibited the highest inhibitory activity against α-amylase; CH against α-glucosidase; and KU against DPPIV and PTP1B enzymes. KA exhibited the highest antioxidant activity (DPPH, FRAP, and ABTS) and greater inhibition of protein glycation compared to other RR indicating its potential to mitigate diabetic complications. The metabolomic analysis confirmed the presence of 99 phenolics in the sample extracts (KU-71, KA-70, CH-68). Molecular docking studies revealed seven metabolites to be good inhibitors of the four target enzymes and activators of insulin receptor substrate/IRS. The antihyperglycemic and oxidation-glycation reduction composite index revealed KA to have the highest overall antidiabetic potential. Hence, the RR could be utilized in functional foods with a multi-barrelled strategy for diabetes prevention/management.Ashrita C. HaldipurNagarajan SrividyaMDPI AGarticleantidiabeticAGEsmetabolomicsphenolic profilemolecular dockingChemical technologyTP1-1185ENFoods, Vol 10, Iss 2818, p 2818 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antidiabetic AGEs metabolomics phenolic profile molecular docking Chemical technology TP1-1185
spellingShingle	antidiabetic AGEs metabolomics phenolic profile molecular docking Chemical technology TP1-1185 Ashrita C. Haldipur Nagarajan Srividya Multi-Mechanistic In Vitro Evaluation of Antihyperglycemic, Antioxidant and Antiglycation Activities of Three Phenolic-Rich Indian Red Rice Genotypes and In Silico Evaluation of Their Phenolic Metabolites
description	The study evaluated the antidiabetic potential of three traditional Indian red rice genotypes/RR (Kattuyanam/KA, Chennangi/CH & Karungkuruvai/KU) using a combination of in vitro, metabolomics (Quadrupole-Time of Flight-Liquid chromatography-Mass spectrometry/Q-TOF-LC-MS/MS)<i>,</i> and in silico techniques. In terms of antihyperglycemic potential, KA exhibited the highest inhibitory activity against α-amylase; CH against α-glucosidase; and KU against DPPIV and PTP1B enzymes. KA exhibited the highest antioxidant activity (DPPH, FRAP, and ABTS) and greater inhibition of protein glycation compared to other RR indicating its potential to mitigate diabetic complications. The metabolomic analysis confirmed the presence of 99 phenolics in the sample extracts (KU-71, KA-70, CH-68). Molecular docking studies revealed seven metabolites to be good inhibitors of the four target enzymes and activators of insulin receptor substrate/IRS. The antihyperglycemic and oxidation-glycation reduction composite index revealed KA to have the highest overall antidiabetic potential. Hence, the RR could be utilized in functional foods with a multi-barrelled strategy for diabetes prevention/management.
format	article
author	Ashrita C. Haldipur Nagarajan Srividya
author_facet	Ashrita C. Haldipur Nagarajan Srividya
author_sort	Ashrita C. Haldipur
title	Multi-Mechanistic In Vitro Evaluation of Antihyperglycemic, Antioxidant and Antiglycation Activities of Three Phenolic-Rich Indian Red Rice Genotypes and In Silico Evaluation of Their Phenolic Metabolites
title_short	Multi-Mechanistic In Vitro Evaluation of Antihyperglycemic, Antioxidant and Antiglycation Activities of Three Phenolic-Rich Indian Red Rice Genotypes and In Silico Evaluation of Their Phenolic Metabolites
title_full	Multi-Mechanistic In Vitro Evaluation of Antihyperglycemic, Antioxidant and Antiglycation Activities of Three Phenolic-Rich Indian Red Rice Genotypes and In Silico Evaluation of Their Phenolic Metabolites
title_fullStr	Multi-Mechanistic In Vitro Evaluation of Antihyperglycemic, Antioxidant and Antiglycation Activities of Three Phenolic-Rich Indian Red Rice Genotypes and In Silico Evaluation of Their Phenolic Metabolites
title_full_unstemmed	Multi-Mechanistic In Vitro Evaluation of Antihyperglycemic, Antioxidant and Antiglycation Activities of Three Phenolic-Rich Indian Red Rice Genotypes and In Silico Evaluation of Their Phenolic Metabolites
title_sort	multi-mechanistic in vitro evaluation of antihyperglycemic, antioxidant and antiglycation activities of three phenolic-rich indian red rice genotypes and in silico evaluation of their phenolic metabolites
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7e4b3cb68a5a4c76b4a3876159dbc1b6
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Multi-Mechanistic In Vitro Evaluation of Antihyperglycemic, Antioxidant and Antiglycation Activities of Three Phenolic-Rich Indian Red Rice Genotypes and In Silico Evaluation of Their Phenolic Metabolites

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