Increased Ratio of CD14++CD80+ Cells/CD14++CD163+ Cells in the Infrapatellar Fat Pad of End-Stage Arthropathy Patients
ObjectivesThis study sought to identify the ratio of M1/M2 cells in the infrapatellar fat pads (IFP) and subcutaneous fat tissues (SC) of osteoarthritis (OA) and rheumatoid arthritis (RA) patients. The clinical features of OA and RA patients treated with or without biological disease-modifying anti-...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:7e4e68273963483fa26e2f37b94f89762021-12-01T07:55:17ZIncreased Ratio of CD14++CD80+ Cells/CD14++CD163+ Cells in the Infrapatellar Fat Pad of End-Stage Arthropathy Patients1664-322410.3389/fimmu.2021.774177https://doaj.org/article/7e4e68273963483fa26e2f37b94f89762021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.774177/fullhttps://doaj.org/toc/1664-3224ObjectivesThis study sought to identify the ratio of M1/M2 cells in the infrapatellar fat pads (IFP) and subcutaneous fat tissues (SC) of osteoarthritis (OA) and rheumatoid arthritis (RA) patients. The clinical features of OA and RA patients treated with or without biological disease-modifying anti-rheumatic drugs (bDMARDs) were also assessed.MethodsIFP and SC were collected from patients with OA and RA who are undergoing total knee arthroplasty (TKA). CD14-positive cells were then isolated from these samples. Flow cytometry was used to determine the number of CD14++CD80+ cells and CD14++CD163+ cells. The expression levels of lipid transcription factors, such as sterol regulatory element-binding protein 1 (SREBP1) and liver X receptor alpha (LXRA), and inflammatory cytokines were also evaluated.ResultsTwenty OA patients and 22 RA patients were enrolled in this study. Ten of the RA patients (45.4%) received bDAMRDs before TKA. On average, a fivefold increase in the number of CD14-positive cells and lower expression levels of SREBP1C and LXRA were observed in OA IFP relative to OA SC; however, these results were not obtained from the RA samples. The median ratio of CD14++CD80+ cells/CD14++CD163+ cells of OA IFP was 0.87 (0.76–1.09, interquartile range), which is higher to that of OA SC with a lower ratio (p = 0.05835).ConclusionsThe quantity and quality of CD14-positive cells differed between IFP and SC in arthropathy patients. To our knowledge, this is the first study to characterize the ratio of M1/M2 cells in the IFP and SC of end-stage OA and RA patients. The increased ratio of CD14++CD80+ cells/CD14++CD163+ cells in the IFP from patients with OA and RA treated with bDMARDs indicated that inflammation was localized in the IFP. As adipose tissue-derived innate immune cells were revealed as one of the targets for regulating inflammation, further analysis of these cells in the IFP may reveal new therapeutic strategies for inflammatory joint diseases.Shuhe MaKosaku MurakamiRintaro SaitoHiromu ItoHiromu ItoKoichi MurataKohei NishitaniMotomu HashimotoMotomu HashimotoMasao TanakaMasahi TaniguchiKoji KitagoriShuji AkizukiRan NakashimaHajime YoshifujiKoichiro OhmuraAkio MorinobuTsuneyo MimoriTsuneyo MimoriFrontiers Media S.A.articleosteoarthritisrheumatoid arthritismonocytes/macrophageinflammationinfrapatellar fat pad (Hoffa’s)Immunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021) |
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| topic |
osteoarthritis rheumatoid arthritis monocytes/macrophage inflammation infrapatellar fat pad (Hoffa’s) Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
osteoarthritis rheumatoid arthritis monocytes/macrophage inflammation infrapatellar fat pad (Hoffa’s) Immunologic diseases. Allergy RC581-607 Shuhe Ma Kosaku Murakami Rintaro Saito Hiromu Ito Hiromu Ito Koichi Murata Kohei Nishitani Motomu Hashimoto Motomu Hashimoto Masao Tanaka Masahi Taniguchi Koji Kitagori Shuji Akizuki Ran Nakashima Hajime Yoshifuji Koichiro Ohmura Akio Morinobu Tsuneyo Mimori Tsuneyo Mimori Increased Ratio of CD14++CD80+ Cells/CD14++CD163+ Cells in the Infrapatellar Fat Pad of End-Stage Arthropathy Patients |
| description |
ObjectivesThis study sought to identify the ratio of M1/M2 cells in the infrapatellar fat pads (IFP) and subcutaneous fat tissues (SC) of osteoarthritis (OA) and rheumatoid arthritis (RA) patients. The clinical features of OA and RA patients treated with or without biological disease-modifying anti-rheumatic drugs (bDMARDs) were also assessed.MethodsIFP and SC were collected from patients with OA and RA who are undergoing total knee arthroplasty (TKA). CD14-positive cells were then isolated from these samples. Flow cytometry was used to determine the number of CD14++CD80+ cells and CD14++CD163+ cells. The expression levels of lipid transcription factors, such as sterol regulatory element-binding protein 1 (SREBP1) and liver X receptor alpha (LXRA), and inflammatory cytokines were also evaluated.ResultsTwenty OA patients and 22 RA patients were enrolled in this study. Ten of the RA patients (45.4%) received bDAMRDs before TKA. On average, a fivefold increase in the number of CD14-positive cells and lower expression levels of SREBP1C and LXRA were observed in OA IFP relative to OA SC; however, these results were not obtained from the RA samples. The median ratio of CD14++CD80+ cells/CD14++CD163+ cells of OA IFP was 0.87 (0.76–1.09, interquartile range), which is higher to that of OA SC with a lower ratio (p = 0.05835).ConclusionsThe quantity and quality of CD14-positive cells differed between IFP and SC in arthropathy patients. To our knowledge, this is the first study to characterize the ratio of M1/M2 cells in the IFP and SC of end-stage OA and RA patients. The increased ratio of CD14++CD80+ cells/CD14++CD163+ cells in the IFP from patients with OA and RA treated with bDMARDs indicated that inflammation was localized in the IFP. As adipose tissue-derived innate immune cells were revealed as one of the targets for regulating inflammation, further analysis of these cells in the IFP may reveal new therapeutic strategies for inflammatory joint diseases. |
| format |
article |
| author |
Shuhe Ma Kosaku Murakami Rintaro Saito Hiromu Ito Hiromu Ito Koichi Murata Kohei Nishitani Motomu Hashimoto Motomu Hashimoto Masao Tanaka Masahi Taniguchi Koji Kitagori Shuji Akizuki Ran Nakashima Hajime Yoshifuji Koichiro Ohmura Akio Morinobu Tsuneyo Mimori Tsuneyo Mimori |
| author_facet |
Shuhe Ma Kosaku Murakami Rintaro Saito Hiromu Ito Hiromu Ito Koichi Murata Kohei Nishitani Motomu Hashimoto Motomu Hashimoto Masao Tanaka Masahi Taniguchi Koji Kitagori Shuji Akizuki Ran Nakashima Hajime Yoshifuji Koichiro Ohmura Akio Morinobu Tsuneyo Mimori Tsuneyo Mimori |
| author_sort |
Shuhe Ma |
| title |
Increased Ratio of CD14++CD80+ Cells/CD14++CD163+ Cells in the Infrapatellar Fat Pad of End-Stage Arthropathy Patients |
| title_short |
Increased Ratio of CD14++CD80+ Cells/CD14++CD163+ Cells in the Infrapatellar Fat Pad of End-Stage Arthropathy Patients |
| title_full |
Increased Ratio of CD14++CD80+ Cells/CD14++CD163+ Cells in the Infrapatellar Fat Pad of End-Stage Arthropathy Patients |
| title_fullStr |
Increased Ratio of CD14++CD80+ Cells/CD14++CD163+ Cells in the Infrapatellar Fat Pad of End-Stage Arthropathy Patients |
| title_full_unstemmed |
Increased Ratio of CD14++CD80+ Cells/CD14++CD163+ Cells in the Infrapatellar Fat Pad of End-Stage Arthropathy Patients |
| title_sort |
increased ratio of cd14++cd80+ cells/cd14++cd163+ cells in the infrapatellar fat pad of end-stage arthropathy patients |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/7e4e68273963483fa26e2f37b94f8976 |
| work_keys_str_mv |
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