Mutation on lysX from Mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotype

The lysX gene from Mycobacterium avium hominissuis (MAH) is not only involved in cationic antimicrobial resistance but also regulates metabolic activity. An MAH lysX deficient mutant was shown to exhibit a metabolic shift at the extracellular state preadapting the bacteria to the conditions inside h...

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Autores principales:	Greana Kirubakar, Hubert Schäfer, Volker Rickerts, Carsten Schwarz, Astrid Lewin
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	virulence mycobacterium avium hominissuis nontuberculous mycobacteria galleria mellonella infection model multinucleated giant cells pathogenesis lysx (lysyl-trna synthetase) host-pathogen interaction inflammatory cytokines Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/7e4ee59fc8f841e286ddfef61d2b6066
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spelling	oai:doaj.org-article:7e4ee59fc8f841e286ddfef61d2b60662021-11-17T14:21:57ZMutation on lysX from Mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotype2150-55942150-560810.1080/21505594.2020.1713690https://doaj.org/article/7e4ee59fc8f841e286ddfef61d2b60662020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1713690https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608The lysX gene from Mycobacterium avium hominissuis (MAH) is not only involved in cationic antimicrobial resistance but also regulates metabolic activity. An MAH lysX deficient mutant was shown to exhibit a metabolic shift at the extracellular state preadapting the bacteria to the conditions inside host-cells. It further showed stronger growth in human monocytes. In the present study, the LysX activity on host–pathogen interactions were analyzed. The lysX mutant from MAH proved to be more sensitive toward host-mediated stresses such as reactive oxygen species. Further, the lysX mutant exhibited increased inflammatory response in PBMC and multinucleated giant cell (MGC) formation in human macrophages during infection studies. Coincidentally, the lysX mutant strain revealed to be more reproductive in the Galleria mellonella infection model. Together, these data demonstrate that LysX plays a role in regulating the bacillary load in host organisms and the lack of lysX gene facilitates MAH adaptation to intracellular host-habitat, thereby suggesting an essential role of LysX in the modulation of host–pathogen interaction.Greana KirubakarHubert SchäferVolker RickertsCarsten SchwarzAstrid LewinTaylor & Francis Grouparticlevirulencemycobacterium avium hominissuisnontuberculous mycobacteriagalleria mellonella infection modelmultinucleated giant cellspathogenesislysx (lysyl-trna synthetase)host-pathogen interactioninflammatory cytokinesInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 132-144 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	virulence mycobacterium avium hominissuis nontuberculous mycobacteria galleria mellonella infection model multinucleated giant cells pathogenesis lysx (lysyl-trna synthetase) host-pathogen interaction inflammatory cytokines Infectious and parasitic diseases RC109-216
spellingShingle	virulence mycobacterium avium hominissuis nontuberculous mycobacteria galleria mellonella infection model multinucleated giant cells pathogenesis lysx (lysyl-trna synthetase) host-pathogen interaction inflammatory cytokines Infectious and parasitic diseases RC109-216 Greana Kirubakar Hubert Schäfer Volker Rickerts Carsten Schwarz Astrid Lewin Mutation on lysX from Mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotype
description	The lysX gene from Mycobacterium avium hominissuis (MAH) is not only involved in cationic antimicrobial resistance but also regulates metabolic activity. An MAH lysX deficient mutant was shown to exhibit a metabolic shift at the extracellular state preadapting the bacteria to the conditions inside host-cells. It further showed stronger growth in human monocytes. In the present study, the LysX activity on host–pathogen interactions were analyzed. The lysX mutant from MAH proved to be more sensitive toward host-mediated stresses such as reactive oxygen species. Further, the lysX mutant exhibited increased inflammatory response in PBMC and multinucleated giant cell (MGC) formation in human macrophages during infection studies. Coincidentally, the lysX mutant strain revealed to be more reproductive in the Galleria mellonella infection model. Together, these data demonstrate that LysX plays a role in regulating the bacillary load in host organisms and the lack of lysX gene facilitates MAH adaptation to intracellular host-habitat, thereby suggesting an essential role of LysX in the modulation of host–pathogen interaction.
format	article
author	Greana Kirubakar Hubert Schäfer Volker Rickerts Carsten Schwarz Astrid Lewin
author_facet	Greana Kirubakar Hubert Schäfer Volker Rickerts Carsten Schwarz Astrid Lewin
author_sort	Greana Kirubakar
title	Mutation on lysX from Mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotype
title_short	Mutation on lysX from Mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotype
title_full	Mutation on lysX from Mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotype
title_fullStr	Mutation on lysX from Mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotype
title_full_unstemmed	Mutation on lysX from Mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotype
title_sort	mutation on lysx from mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotype
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/7e4ee59fc8f841e286ddfef61d2b6066
work_keys_str_mv	AT greanakirubakar mutationonlysxfrommycobacteriumaviumhominissuisimpactsthehostpathogeninteractionandvirulencephenotype AT hubertschafer mutationonlysxfrommycobacteriumaviumhominissuisimpactsthehostpathogeninteractionandvirulencephenotype AT volkerrickerts mutationonlysxfrommycobacteriumaviumhominissuisimpactsthehostpathogeninteractionandvirulencephenotype AT carstenschwarz mutationonlysxfrommycobacteriumaviumhominissuisimpactsthehostpathogeninteractionandvirulencephenotype AT astridlewin mutationonlysxfrommycobacteriumaviumhominissuisimpactsthehostpathogeninteractionandvirulencephenotype
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Mutation on lysX from Mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotype

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