Potential inhibition of HIV-1 encapsidation by oligoribonucleotide–dendrimer nanoparticle complexes
Raveen Parboosing,1,2 Louis Chonco,1,2 Francisco Javier de la Mata,3,4 Thavendran Govender,5 Glenn EM Maguire,5 Hendrik G Kruger5 1Department of Virology, University of KwaZulu-Natal, 2National Health Laboratory Service, Durban, South Africa; 3Organic and Inorganic Chemistry Department, University...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/7e51b875d143498289c5dc1084ecedbf |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:7e51b875d143498289c5dc1084ecedbf |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:7e51b875d143498289c5dc1084ecedbf2021-12-02T03:58:39ZPotential inhibition of HIV-1 encapsidation by oligoribonucleotide–dendrimer nanoparticle complexes1178-2013https://doaj.org/article/7e51b875d143498289c5dc1084ecedbf2017-01-01T00:00:00Zhttps://www.dovepress.com/potential-inhibition-of-hiv-1-encapsidation-by-oligoribonucleotide-den-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Raveen Parboosing,1,2 Louis Chonco,1,2 Francisco Javier de la Mata,3,4 Thavendran Govender,5 Glenn EM Maguire,5 Hendrik G Kruger5 1Department of Virology, University of KwaZulu-Natal, 2National Health Laboratory Service, Durban, South Africa; 3Organic and Inorganic Chemistry Department, University of Alcalá, Alcalá de Henares, 4Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; 5Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Durban, South Africa Background: Encapsidation, the process during which the genomic RNA of HIV is packaged into viral particles, is an attractive target for antiviral therapy. This study explores a novel nanotechnology-based strategy to inhibit HIV encapsidation by an RNA decoy mechanism. The design of the 16-mer oligoribonucleotide (RNA) decoy is based on the sequence of stem loop 3 (SL3) of the HIV packaging signal (Ψ). Recognition of the packaging signal is essential to the encapsidation process. It is theorized that the decoy RNA, by mimicking the packaging signal, will disrupt HIV packaging if efficiently delivered into lymphocytes by complexation with a carbosilane dendrimer. The aim of the study is to measure the uptake, toxicity, and antiviral activity of the dendrimer–RNA nanocomplex. Materials and methods: A dendriplex was formed between cationic carbosilane dendrimers and the RNA decoy. Uptake of the fluorescein-labeled RNA into MT4 lymphocytes was determined by flow cytometry and confocal microscopy. The cytoprotective effect (50% effective concentration [EC50]) and the effect on HIV replication were determined in vitro by the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and viral load measurements, respectively. Results: Flow cytometry and confocal imaging demonstrated efficient transfection of lymphocytes. The dendriplex containing the Ψ decoy showed some activity (EC50 =3.20 µM, selectivity index =8.4). However, there was no significant suppression of HIV viral load. Conclusion: Oligoribonucleotide decoys containing SL3 of the packaging sequence are efficiently delivered into lymphocytes by carbosilane dendrimers where they exhibit a modest cytoprotective effect against HIV infection. Keywords: packaging signal, dendrimers, transfection, antiretroviral, HIV packagingParboosing RChonco Lde la Mata FJGovender TMaguire GEMKruger HGDove Medical PressarticlePackaging signaldendrimerstransfectionantiretroviralHIV packagingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 317-325 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Packaging signal dendrimers transfection antiretroviral HIV packaging Medicine (General) R5-920 |
| spellingShingle |
Packaging signal dendrimers transfection antiretroviral HIV packaging Medicine (General) R5-920 Parboosing R Chonco L de la Mata FJ Govender T Maguire GEM Kruger HG Potential inhibition of HIV-1 encapsidation by oligoribonucleotide–dendrimer nanoparticle complexes |
| description |
Raveen Parboosing,1,2 Louis Chonco,1,2 Francisco Javier de la Mata,3,4 Thavendran Govender,5 Glenn EM Maguire,5 Hendrik G Kruger5 1Department of Virology, University of KwaZulu-Natal, 2National Health Laboratory Service, Durban, South Africa; 3Organic and Inorganic Chemistry Department, University of Alcalá, Alcalá de Henares, 4Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain; 5Catalysis and Peptide Research Unit, University of KwaZulu-Natal, Durban, South Africa Background: Encapsidation, the process during which the genomic RNA of HIV is packaged into viral particles, is an attractive target for antiviral therapy. This study explores a novel nanotechnology-based strategy to inhibit HIV encapsidation by an RNA decoy mechanism. The design of the 16-mer oligoribonucleotide (RNA) decoy is based on the sequence of stem loop 3 (SL3) of the HIV packaging signal (Ψ). Recognition of the packaging signal is essential to the encapsidation process. It is theorized that the decoy RNA, by mimicking the packaging signal, will disrupt HIV packaging if efficiently delivered into lymphocytes by complexation with a carbosilane dendrimer. The aim of the study is to measure the uptake, toxicity, and antiviral activity of the dendrimer–RNA nanocomplex. Materials and methods: A dendriplex was formed between cationic carbosilane dendrimers and the RNA decoy. Uptake of the fluorescein-labeled RNA into MT4 lymphocytes was determined by flow cytometry and confocal microscopy. The cytoprotective effect (50% effective concentration [EC50]) and the effect on HIV replication were determined in vitro by the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and viral load measurements, respectively. Results: Flow cytometry and confocal imaging demonstrated efficient transfection of lymphocytes. The dendriplex containing the Ψ decoy showed some activity (EC50 =3.20 µM, selectivity index =8.4). However, there was no significant suppression of HIV viral load. Conclusion: Oligoribonucleotide decoys containing SL3 of the packaging sequence are efficiently delivered into lymphocytes by carbosilane dendrimers where they exhibit a modest cytoprotective effect against HIV infection. Keywords: packaging signal, dendrimers, transfection, antiretroviral, HIV packaging |
| format |
article |
| author |
Parboosing R Chonco L de la Mata FJ Govender T Maguire GEM Kruger HG |
| author_facet |
Parboosing R Chonco L de la Mata FJ Govender T Maguire GEM Kruger HG |
| author_sort |
Parboosing R |
| title |
Potential inhibition of HIV-1 encapsidation by oligoribonucleotide–dendrimer nanoparticle complexes |
| title_short |
Potential inhibition of HIV-1 encapsidation by oligoribonucleotide–dendrimer nanoparticle complexes |
| title_full |
Potential inhibition of HIV-1 encapsidation by oligoribonucleotide–dendrimer nanoparticle complexes |
| title_fullStr |
Potential inhibition of HIV-1 encapsidation by oligoribonucleotide–dendrimer nanoparticle complexes |
| title_full_unstemmed |
Potential inhibition of HIV-1 encapsidation by oligoribonucleotide–dendrimer nanoparticle complexes |
| title_sort |
potential inhibition of hiv-1 encapsidation by oligoribonucleotide–dendrimer nanoparticle complexes |
| publisher |
Dove Medical Press |
| publishDate |
2017 |
| url |
https://doaj.org/article/7e51b875d143498289c5dc1084ecedbf |
| work_keys_str_mv |
AT parboosingr potentialinhibitionofhiv1encapsidationbyoligoribonucleotidendashdendrimernanoparticlecomplexes AT choncol potentialinhibitionofhiv1encapsidationbyoligoribonucleotidendashdendrimernanoparticlecomplexes AT delamatafj potentialinhibitionofhiv1encapsidationbyoligoribonucleotidendashdendrimernanoparticlecomplexes AT govendert potentialinhibitionofhiv1encapsidationbyoligoribonucleotidendashdendrimernanoparticlecomplexes AT maguiregem potentialinhibitionofhiv1encapsidationbyoligoribonucleotidendashdendrimernanoparticlecomplexes AT krugerhg potentialinhibitionofhiv1encapsidationbyoligoribonucleotidendashdendrimernanoparticlecomplexes |
| _version_ |
1718401499847983104 |