Voronoi tessellation captures very early clustering of single primary cells as induced by interactions in nascent biofilms.

Biofilms dominate microbial life in numerous aquatic ecosystems, and in engineered and medical systems, as well. The formation of biofilms is initiated by single primary cells colonizing surfaces from the bulk liquid. The next steps from primary cells towards the first cell clusters as the initial s...

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Autores principales:	Iris Hödl, Josef Hödl, Anders Wörman, Gabriel Singer, Katharina Besemer, Tom J Battin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e57538e6f0a4338be88b318eeb98e2f
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spelling	oai:doaj.org-article:7e57538e6f0a4338be88b318eeb98e2f2021-11-18T07:36:16ZVoronoi tessellation captures very early clustering of single primary cells as induced by interactions in nascent biofilms.1932-620310.1371/journal.pone.0026368https://doaj.org/article/7e57538e6f0a4338be88b318eeb98e2f2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22028865/?tool=EBIhttps://doaj.org/toc/1932-6203Biofilms dominate microbial life in numerous aquatic ecosystems, and in engineered and medical systems, as well. The formation of biofilms is initiated by single primary cells colonizing surfaces from the bulk liquid. The next steps from primary cells towards the first cell clusters as the initial step of biofilm formation remain relatively poorly studied. Clonal growth and random migration of primary cells are traditionally considered as the dominant processes leading to organized microcolonies in laboratory grown monocultures. Using Voronoi tessellation, we show that the spatial distribution of primary cells colonizing initially sterile surfaces from natural streamwater community deviates from uniform randomness already during the very early colonisation. The deviation from uniform randomness increased with colonisation--despite the absence of cell reproduction--and was even more pronounced when the flow of water above biofilms was multidirectional and shear stress elevated. We propose a simple mechanistic model that captures interactions, such as cell-to-cell signalling or chemical surface conditioning, to simulate the observed distribution patterns. Model predictions match empirical observations reasonably well, highlighting the role of biotic interactions even already during very early biofilm formation despite few and distant cells. The transition from single primary cells to clustering accelerated by biotic interactions rather than by reproduction may be particularly advantageous in harsh environments--the rule rather than the exception outside the laboratory.Iris HödlJosef HödlAnders WörmanGabriel SingerKatharina BesemerTom J BattinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26368 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Iris Hödl Josef Hödl Anders Wörman Gabriel Singer Katharina Besemer Tom J Battin Voronoi tessellation captures very early clustering of single primary cells as induced by interactions in nascent biofilms.
description	Biofilms dominate microbial life in numerous aquatic ecosystems, and in engineered and medical systems, as well. The formation of biofilms is initiated by single primary cells colonizing surfaces from the bulk liquid. The next steps from primary cells towards the first cell clusters as the initial step of biofilm formation remain relatively poorly studied. Clonal growth and random migration of primary cells are traditionally considered as the dominant processes leading to organized microcolonies in laboratory grown monocultures. Using Voronoi tessellation, we show that the spatial distribution of primary cells colonizing initially sterile surfaces from natural streamwater community deviates from uniform randomness already during the very early colonisation. The deviation from uniform randomness increased with colonisation--despite the absence of cell reproduction--and was even more pronounced when the flow of water above biofilms was multidirectional and shear stress elevated. We propose a simple mechanistic model that captures interactions, such as cell-to-cell signalling or chemical surface conditioning, to simulate the observed distribution patterns. Model predictions match empirical observations reasonably well, highlighting the role of biotic interactions even already during very early biofilm formation despite few and distant cells. The transition from single primary cells to clustering accelerated by biotic interactions rather than by reproduction may be particularly advantageous in harsh environments--the rule rather than the exception outside the laboratory.
format	article
author	Iris Hödl Josef Hödl Anders Wörman Gabriel Singer Katharina Besemer Tom J Battin
author_facet	Iris Hödl Josef Hödl Anders Wörman Gabriel Singer Katharina Besemer Tom J Battin
author_sort	Iris Hödl
title	Voronoi tessellation captures very early clustering of single primary cells as induced by interactions in nascent biofilms.
title_short	Voronoi tessellation captures very early clustering of single primary cells as induced by interactions in nascent biofilms.
title_full	Voronoi tessellation captures very early clustering of single primary cells as induced by interactions in nascent biofilms.
title_fullStr	Voronoi tessellation captures very early clustering of single primary cells as induced by interactions in nascent biofilms.
title_full_unstemmed	Voronoi tessellation captures very early clustering of single primary cells as induced by interactions in nascent biofilms.
title_sort	voronoi tessellation captures very early clustering of single primary cells as induced by interactions in nascent biofilms.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/7e57538e6f0a4338be88b318eeb98e2f
work_keys_str_mv	AT irishodl voronoitessellationcapturesveryearlyclusteringofsingleprimarycellsasinducedbyinteractionsinnascentbiofilms AT josefhodl voronoitessellationcapturesveryearlyclusteringofsingleprimarycellsasinducedbyinteractionsinnascentbiofilms AT andersworman voronoitessellationcapturesveryearlyclusteringofsingleprimarycellsasinducedbyinteractionsinnascentbiofilms AT gabrielsinger voronoitessellationcapturesveryearlyclusteringofsingleprimarycellsasinducedbyinteractionsinnascentbiofilms AT katharinabesemer voronoitessellationcapturesveryearlyclusteringofsingleprimarycellsasinducedbyinteractionsinnascentbiofilms AT tomjbattin voronoitessellationcapturesveryearlyclusteringofsingleprimarycellsasinducedbyinteractionsinnascentbiofilms
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Voronoi tessellation captures very early clustering of single primary cells as induced by interactions in nascent biofilms.

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