A partially demineralized allogeneic bone graft: in vitro osteogenic potential and preclinical evaluation in two different intramembranous bone healing models

Abstract In skeletal surgical procedures, bone regeneration in irregular and hard-to-reach areas may present clinical challenges. In order to overcome the limitations of traditional autologous bone grafts and bone substitutes, an extrudable and easy-to-handle innovative partially demineralized allog...

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Autores principales:	Pierre Tournier, Jérôme Guicheux, Arnaud Paré, Aymeric Maltezeanu, Thibaut Blondy, Joëlle Veziers, Caroline Vignes, Manon André, Julie Lesoeur, Ana Barbeito, Raphaël Bardonnet, Christophe Blanquart, Pierre Corre, Valérie Geoffroy, Pierre Weiss, Alexis Gaudin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e57b958d5f045e599b25244a0048b33
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spelling	oai:doaj.org-article:7e57b958d5f045e599b25244a0048b332021-12-02T13:30:10ZA partially demineralized allogeneic bone graft: in vitro osteogenic potential and preclinical evaluation in two different intramembranous bone healing models10.1038/s41598-021-84039-62045-2322https://doaj.org/article/7e57b958d5f045e599b25244a0048b332021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84039-6https://doaj.org/toc/2045-2322Abstract In skeletal surgical procedures, bone regeneration in irregular and hard-to-reach areas may present clinical challenges. In order to overcome the limitations of traditional autologous bone grafts and bone substitutes, an extrudable and easy-to-handle innovative partially demineralized allogenic bone graft in the form of a paste has been developed. In this study, the regenerative potential of this paste was assessed and compared to its clinically used precursor form allogenic bone particles. Compared to the particular bone graft, the bone paste allowed better attachment of human mesenchymal stromal cells and their commitment towards the osteoblastic lineage, and it induced a pro-regenerative phenotype of human monocytes/macrophages. The bone paste also supported bone healing in vivo in a guide bone regeneration model and, more interestingly, exhibited a substantial bone-forming ability when implanted in a critical-size defect model in rat calvaria. Thus, these findings indicate that this novel partially demineralized allogeneic bone paste that combines substantial bone healing properties and rapid and ease-of-use may be a promising alternative to allogeneic bone grafts for bone regeneration in several clinical contexts of oral and maxillofacial bone grafting.Pierre TournierJérôme GuicheuxArnaud ParéAymeric MaltezeanuThibaut BlondyJoëlle VeziersCaroline VignesManon AndréJulie LesoeurAna BarbeitoRaphaël BardonnetChristophe BlanquartPierre CorreValérie GeoffroyPierre WeissAlexis GaudinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pierre Tournier Jérôme Guicheux Arnaud Paré Aymeric Maltezeanu Thibaut Blondy Joëlle Veziers Caroline Vignes Manon André Julie Lesoeur Ana Barbeito Raphaël Bardonnet Christophe Blanquart Pierre Corre Valérie Geoffroy Pierre Weiss Alexis Gaudin A partially demineralized allogeneic bone graft: in vitro osteogenic potential and preclinical evaluation in two different intramembranous bone healing models
description	Abstract In skeletal surgical procedures, bone regeneration in irregular and hard-to-reach areas may present clinical challenges. In order to overcome the limitations of traditional autologous bone grafts and bone substitutes, an extrudable and easy-to-handle innovative partially demineralized allogenic bone graft in the form of a paste has been developed. In this study, the regenerative potential of this paste was assessed and compared to its clinically used precursor form allogenic bone particles. Compared to the particular bone graft, the bone paste allowed better attachment of human mesenchymal stromal cells and their commitment towards the osteoblastic lineage, and it induced a pro-regenerative phenotype of human monocytes/macrophages. The bone paste also supported bone healing in vivo in a guide bone regeneration model and, more interestingly, exhibited a substantial bone-forming ability when implanted in a critical-size defect model in rat calvaria. Thus, these findings indicate that this novel partially demineralized allogeneic bone paste that combines substantial bone healing properties and rapid and ease-of-use may be a promising alternative to allogeneic bone grafts for bone regeneration in several clinical contexts of oral and maxillofacial bone grafting.
format	article
author	Pierre Tournier Jérôme Guicheux Arnaud Paré Aymeric Maltezeanu Thibaut Blondy Joëlle Veziers Caroline Vignes Manon André Julie Lesoeur Ana Barbeito Raphaël Bardonnet Christophe Blanquart Pierre Corre Valérie Geoffroy Pierre Weiss Alexis Gaudin
author_facet	Pierre Tournier Jérôme Guicheux Arnaud Paré Aymeric Maltezeanu Thibaut Blondy Joëlle Veziers Caroline Vignes Manon André Julie Lesoeur Ana Barbeito Raphaël Bardonnet Christophe Blanquart Pierre Corre Valérie Geoffroy Pierre Weiss Alexis Gaudin
author_sort	Pierre Tournier
title	A partially demineralized allogeneic bone graft: in vitro osteogenic potential and preclinical evaluation in two different intramembranous bone healing models
title_short	A partially demineralized allogeneic bone graft: in vitro osteogenic potential and preclinical evaluation in two different intramembranous bone healing models
title_full	A partially demineralized allogeneic bone graft: in vitro osteogenic potential and preclinical evaluation in two different intramembranous bone healing models
title_fullStr	A partially demineralized allogeneic bone graft: in vitro osteogenic potential and preclinical evaluation in two different intramembranous bone healing models
title_full_unstemmed	A partially demineralized allogeneic bone graft: in vitro osteogenic potential and preclinical evaluation in two different intramembranous bone healing models
title_sort	partially demineralized allogeneic bone graft: in vitro osteogenic potential and preclinical evaluation in two different intramembranous bone healing models
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7e57b958d5f045e599b25244a0048b33
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A partially demineralized allogeneic bone graft: in vitro osteogenic potential and preclinical evaluation in two different intramembranous bone healing models

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