Choosing source of microorganisms and processing technology for next generation beet bioinoculant

Abstract The increase of human population and associated increasing demand for agricultural products lead to soil over-exploitation. Biofertilizers based on lyophilized plant material containing living plant growth-promoting microorganisms (PGPM) could be an alternative to conventional fertilizers t...

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Autores principales:	Sonia Szymańska, Marcin Sikora, Katarzyna Hrynkiewicz, Jarosław Tyburski, Andrzej Tretyn, Marcin Gołębiewski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e5b1fa628064308920c5350a298cc60
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spelling	oai:doaj.org-article:7e5b1fa628064308920c5350a298cc602021-12-02T14:06:12ZChoosing source of microorganisms and processing technology for next generation beet bioinoculant10.1038/s41598-021-82436-52045-2322https://doaj.org/article/7e5b1fa628064308920c5350a298cc602021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82436-5https://doaj.org/toc/2045-2322Abstract The increase of human population and associated increasing demand for agricultural products lead to soil over-exploitation. Biofertilizers based on lyophilized plant material containing living plant growth-promoting microorganisms (PGPM) could be an alternative to conventional fertilizers that fits into sustainable agricultural technologies ideas. We aimed to: (1) assess the diversity of endophytic bacteria in sugar and sea beet roots and (2) determine the influence of osmoprotectants (trehalose and ectoine) addition during lyophilization on bacterial density, viability and salt tolerance. Microbiome diversity was assessed based on 16S rRNA amplicons sequencing, bacterial density and salt tolerance was evaluated in cultures, while bacterial viability was calculated by using fluorescence microscopy and flow cytometry. Here we show that plant genotype shapes its endophytic microbiome diversity and determines rhizosphere soil properties. Sea beet endophytic microbiome, consisting of genera characteristic for extreme environments, is more diverse and salt resistant than its crop relative. Supplementing osmoprotectants during root tissue lyophilization exerts a positive effect on bacterial community salt stress tolerance, viability and density. Trehalose improves the above-mentioned parameters more effectively than ectoine, moreover its use is economically advantageous, thus it may be used to formulate improved biofertilizers.Sonia SzymańskaMarcin SikoraKatarzyna HrynkiewiczJarosław TyburskiAndrzej TretynMarcin GołębiewskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sonia Szymańska Marcin Sikora Katarzyna Hrynkiewicz Jarosław Tyburski Andrzej Tretyn Marcin Gołębiewski Choosing source of microorganisms and processing technology for next generation beet bioinoculant
description	Abstract The increase of human population and associated increasing demand for agricultural products lead to soil over-exploitation. Biofertilizers based on lyophilized plant material containing living plant growth-promoting microorganisms (PGPM) could be an alternative to conventional fertilizers that fits into sustainable agricultural technologies ideas. We aimed to: (1) assess the diversity of endophytic bacteria in sugar and sea beet roots and (2) determine the influence of osmoprotectants (trehalose and ectoine) addition during lyophilization on bacterial density, viability and salt tolerance. Microbiome diversity was assessed based on 16S rRNA amplicons sequencing, bacterial density and salt tolerance was evaluated in cultures, while bacterial viability was calculated by using fluorescence microscopy and flow cytometry. Here we show that plant genotype shapes its endophytic microbiome diversity and determines rhizosphere soil properties. Sea beet endophytic microbiome, consisting of genera characteristic for extreme environments, is more diverse and salt resistant than its crop relative. Supplementing osmoprotectants during root tissue lyophilization exerts a positive effect on bacterial community salt stress tolerance, viability and density. Trehalose improves the above-mentioned parameters more effectively than ectoine, moreover its use is economically advantageous, thus it may be used to formulate improved biofertilizers.
format	article
author	Sonia Szymańska Marcin Sikora Katarzyna Hrynkiewicz Jarosław Tyburski Andrzej Tretyn Marcin Gołębiewski
author_facet	Sonia Szymańska Marcin Sikora Katarzyna Hrynkiewicz Jarosław Tyburski Andrzej Tretyn Marcin Gołębiewski
author_sort	Sonia Szymańska
title	Choosing source of microorganisms and processing technology for next generation beet bioinoculant
title_short	Choosing source of microorganisms and processing technology for next generation beet bioinoculant
title_full	Choosing source of microorganisms and processing technology for next generation beet bioinoculant
title_fullStr	Choosing source of microorganisms and processing technology for next generation beet bioinoculant
title_full_unstemmed	Choosing source of microorganisms and processing technology for next generation beet bioinoculant
title_sort	choosing source of microorganisms and processing technology for next generation beet bioinoculant
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7e5b1fa628064308920c5350a298cc60
work_keys_str_mv	AT soniaszymanska choosingsourceofmicroorganismsandprocessingtechnologyfornextgenerationbeetbioinoculant AT marcinsikora choosingsourceofmicroorganismsandprocessingtechnologyfornextgenerationbeetbioinoculant AT katarzynahrynkiewicz choosingsourceofmicroorganismsandprocessingtechnologyfornextgenerationbeetbioinoculant AT jarosławtyburski choosingsourceofmicroorganismsandprocessingtechnologyfornextgenerationbeetbioinoculant AT andrzejtretyn choosingsourceofmicroorganismsandprocessingtechnologyfornextgenerationbeetbioinoculant AT marcingołebiewski choosingsourceofmicroorganismsandprocessingtechnologyfornextgenerationbeetbioinoculant
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Choosing source of microorganisms and processing technology for next generation beet bioinoculant

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