Nanoscale bacteriophage biosensors beyond phage display

Jong-Wook Lee,1 Jangwon Song,1,2 Mintai P Hwang,1 Kwan Hyi Lee1,2 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Korea; 2Department of Biomedical Engineering, University of Science and Technology, Seoul, Korea Abstract: Bacteriophages are t...

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Autores principales:	Lee JW, Song J, Hwang MP, Lee KH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7e5c96eb57854fcd9ceb15c1a71166e7
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spelling	oai:doaj.org-article:7e5c96eb57854fcd9ceb15c1a71166e72021-12-02T02:42:33ZNanoscale bacteriophage biosensors beyond phage display1176-91141178-2013https://doaj.org/article/7e5c96eb57854fcd9ceb15c1a71166e72013-10-01T00:00:00Zhttp://www.dovepress.com/nanoscale-bacteriophage-biosensors-beyond-phage-display-a14644https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jong-Wook Lee,1 Jangwon Song,1,2 Mintai P Hwang,1 Kwan Hyi Lee1,2 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Korea; 2Department of Biomedical Engineering, University of Science and Technology, Seoul, Korea Abstract: Bacteriophages are traditionally used for the development of phage display technology. Recently, their nanosized dimensions and ease with which genetic modifications can be made to their structure and function have put them in the spotlight towards their use in a variety of biosensors. In particular, the expression of any protein or peptide on the extraluminal surface of bacteriophages is possible by genetically engineering the genome. In addition, the relatively short replication time of bacteriophages offers researchers the ability to generate mass quantities of any given bacteriophage-based biosensor. Coupled with the emergence of various biomarkers in the clinic as a means to determine pathophysiological states, the development of current and novel technologies for their detection and quantification is imperative. In this review, we categorize bacteriophages by their morphology into M13-based filamentous bacteriophages and T4- or T7-based icosahedral bacteriophages, and examine how such advantages are utilized across a variety of biosensors. In essence, we take a comprehensive approach towards recent trends in bacteriophage-based biosensor applications and discuss their outlook with regards to the field of biotechnology. Keywords: biosensing, M13 bacteriophage, T4 bacteriophage, bacterial detection, Escherichia coli, SPR sensorLee JWSong JHwang MPLee KHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 3917-3925 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lee JW Song J Hwang MP Lee KH Nanoscale bacteriophage biosensors beyond phage display
description	Jong-Wook Lee,1 Jangwon Song,1,2 Mintai P Hwang,1 Kwan Hyi Lee1,2 1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Korea; 2Department of Biomedical Engineering, University of Science and Technology, Seoul, Korea Abstract: Bacteriophages are traditionally used for the development of phage display technology. Recently, their nanosized dimensions and ease with which genetic modifications can be made to their structure and function have put them in the spotlight towards their use in a variety of biosensors. In particular, the expression of any protein or peptide on the extraluminal surface of bacteriophages is possible by genetically engineering the genome. In addition, the relatively short replication time of bacteriophages offers researchers the ability to generate mass quantities of any given bacteriophage-based biosensor. Coupled with the emergence of various biomarkers in the clinic as a means to determine pathophysiological states, the development of current and novel technologies for their detection and quantification is imperative. In this review, we categorize bacteriophages by their morphology into M13-based filamentous bacteriophages and T4- or T7-based icosahedral bacteriophages, and examine how such advantages are utilized across a variety of biosensors. In essence, we take a comprehensive approach towards recent trends in bacteriophage-based biosensor applications and discuss their outlook with regards to the field of biotechnology. Keywords: biosensing, M13 bacteriophage, T4 bacteriophage, bacterial detection, Escherichia coli, SPR sensor
format	article
author	Lee JW Song J Hwang MP Lee KH
author_facet	Lee JW Song J Hwang MP Lee KH
author_sort	Lee JW
title	Nanoscale bacteriophage biosensors beyond phage display
title_short	Nanoscale bacteriophage biosensors beyond phage display
title_full	Nanoscale bacteriophage biosensors beyond phage display
title_fullStr	Nanoscale bacteriophage biosensors beyond phage display
title_full_unstemmed	Nanoscale bacteriophage biosensors beyond phage display
title_sort	nanoscale bacteriophage biosensors beyond phage display
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/7e5c96eb57854fcd9ceb15c1a71166e7
work_keys_str_mv	AT leejw nanoscalebacteriophagebiosensorsbeyondphagedisplay AT songj nanoscalebacteriophagebiosensorsbeyondphagedisplay AT hwangmp nanoscalebacteriophagebiosensorsbeyondphagedisplay AT leekh nanoscalebacteriophagebiosensorsbeyondphagedisplay
_version_	1718402229278343168

Nanoscale bacteriophage biosensors beyond phage display

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