The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity

Abstract The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is the most common method for the determination of cell toxicity, but some factors limit the sensitivity of this method, such as pH. Less attention had been paid to the interference effect of optical and plasmonic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Fariba Abbasi, Hassan Hashemi, Mohammad Reza Samaei, Amir SavarDashtaki, Abooalfazl Azhdarpoor, Mohammad Javad Fallahi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e5e823b6cca45aba4e73a7d04b7e8bc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7e5e823b6cca45aba4e73a7d04b7e8bc
record_format	dspace
spelling	oai:doaj.org-article:7e5e823b6cca45aba4e73a7d04b7e8bc2021-12-02T16:17:17ZThe synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity10.1038/s41598-021-92419-12045-2322https://doaj.org/article/7e5e823b6cca45aba4e73a7d04b7e8bc2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92419-1https://doaj.org/toc/2045-2322Abstract The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is the most common method for the determination of cell toxicity, but some factors limit the sensitivity of this method, such as pH. Less attention had been paid to the interference effect of optical and plasmonic properties of SiO2 nanoparticles (NPs) in the wavelength range assigned to MTT. This study investigated the synergistic interference effect of SiO2 NPs and wavelength on MTT assay for the first time. The examined variables included the type of SiO2 NPs concentrations (1, 10, and 100 mM) and different wavelengths (470, 490, 520, and 570 nm). The results showed that optical density (OD) increased (p < 0.05) when wavelength and the concentration of crystalline SiO2 NPs increased. So, the maximum OD at 10 and 100 mM were attributed to crystalline SiO2 NPs (p < 0.05) due to the functional group, whereas it was related to amorphous at 1 mM (p > 0.05). According to polynomial regression modeling (PRM), the maximum interference effect was predicted at crystalline SiO2 NPs and wavelength > 550 nm. Besides, the synergistic effects of SiO2 NPs, wavelength, and concentration of NPs had been a good fitting with first-order PRM. Thus, the concentration of SiO2 NPs had a confounder factor in colorimetric for MTT assay. The best artificial neural network (ANN) structure was related to the 3:7:1 network (Rall = 0.936, MSE = 0.0006, MAPE = 0.063). The correlation between the actual and predicted data was 0.88. As SiO2 NPs presence is an interfering factor in MTT assay concerning wavelength, it is suggested wavelength use with minimum confounding effect for MTT assay.Fariba AbbasiHassan HashemiMohammad Reza SamaeiAmir SavarDashtakiAbooalfazl AzhdarpoorMohammad Javad FallahiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fariba Abbasi Hassan Hashemi Mohammad Reza Samaei Amir SavarDashtaki Abooalfazl Azhdarpoor Mohammad Javad Fallahi The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity
description	Abstract The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is the most common method for the determination of cell toxicity, but some factors limit the sensitivity of this method, such as pH. Less attention had been paid to the interference effect of optical and plasmonic properties of SiO2 nanoparticles (NPs) in the wavelength range assigned to MTT. This study investigated the synergistic interference effect of SiO2 NPs and wavelength on MTT assay for the first time. The examined variables included the type of SiO2 NPs concentrations (1, 10, and 100 mM) and different wavelengths (470, 490, 520, and 570 nm). The results showed that optical density (OD) increased (p < 0.05) when wavelength and the concentration of crystalline SiO2 NPs increased. So, the maximum OD at 10 and 100 mM were attributed to crystalline SiO2 NPs (p < 0.05) due to the functional group, whereas it was related to amorphous at 1 mM (p > 0.05). According to polynomial regression modeling (PRM), the maximum interference effect was predicted at crystalline SiO2 NPs and wavelength > 550 nm. Besides, the synergistic effects of SiO2 NPs, wavelength, and concentration of NPs had been a good fitting with first-order PRM. Thus, the concentration of SiO2 NPs had a confounder factor in colorimetric for MTT assay. The best artificial neural network (ANN) structure was related to the 3:7:1 network (Rall = 0.936, MSE = 0.0006, MAPE = 0.063). The correlation between the actual and predicted data was 0.88. As SiO2 NPs presence is an interfering factor in MTT assay concerning wavelength, it is suggested wavelength use with minimum confounding effect for MTT assay.
format	article
author	Fariba Abbasi Hassan Hashemi Mohammad Reza Samaei Amir SavarDashtaki Abooalfazl Azhdarpoor Mohammad Javad Fallahi
author_facet	Fariba Abbasi Hassan Hashemi Mohammad Reza Samaei Amir SavarDashtaki Abooalfazl Azhdarpoor Mohammad Javad Fallahi
author_sort	Fariba Abbasi
title	The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity
title_short	The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity
title_full	The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity
title_fullStr	The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity
title_full_unstemmed	The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity
title_sort	synergistic interference effect of silica nanoparticles concentration and the wavelength of elisa on the colorimetric assay of cell toxicity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7e5e823b6cca45aba4e73a7d04b7e8bc
work_keys_str_mv	AT faribaabbasi thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT hassanhashemi thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT mohammadrezasamaei thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT amirsavardashtaki thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT abooalfazlazhdarpoor thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT mohammadjavadfallahi thesynergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT faribaabbasi synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT hassanhashemi synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT mohammadrezasamaei synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT amirsavardashtaki synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT abooalfazlazhdarpoor synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity AT mohammadjavadfallahi synergisticinterferenceeffectofsilicananoparticlesconcentrationandthewavelengthofelisaonthecolorimetricassayofcelltoxicity
_version_	1718384239484862464

The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity

Ejemplares similares