Cell fusion reprogramming leads to a specific hepatic expression pattern during mouse bone marrow derived hepatocyte formation in vivo.
The fusion of bone marrow (BM) hematopoietic cells with hepatocytes to generate BM derived hepatocytes (BMDH) is a natural process, which is enhanced in damaged tissues. However, the reprogramming needed to generate BMDH and the identity of the resultant cells is essentially unknown. In a mouse mode...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/7e5ee3b5815344a4ae5686ff3487d995 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:7e5ee3b5815344a4ae5686ff3487d995 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:7e5ee3b5815344a4ae5686ff3487d9952021-11-18T07:24:24ZCell fusion reprogramming leads to a specific hepatic expression pattern during mouse bone marrow derived hepatocyte formation in vivo.1932-620310.1371/journal.pone.0033945https://doaj.org/article/7e5ee3b5815344a4ae5686ff3487d9952012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22457803/?tool=EBIhttps://doaj.org/toc/1932-6203The fusion of bone marrow (BM) hematopoietic cells with hepatocytes to generate BM derived hepatocytes (BMDH) is a natural process, which is enhanced in damaged tissues. However, the reprogramming needed to generate BMDH and the identity of the resultant cells is essentially unknown. In a mouse model of chronic liver damage, here we identify a modification in the chromatin structure of the hematopoietic nucleus during BMDH formation, accompanied by the loss of the key hematopoietic transcription factor PU.1/Sfpi1 (SFFV proviral integration 1) and gain of the key hepatic transcriptional regulator HNF-1A homeobox A (HNF-1A/Hnf1a). Through genome-wide expression analysis of laser captured BMDH, a differential gene expression pattern was detected and the chromatin changes observed were confirmed at the level of chromatin regulator genes. Similarly, Tranforming Growth Factor-β1 (TGF-β(1)) and neurotransmitter (e.g. Prostaglandin E Receptor 4 [Ptger4]) pathway genes were over-expressed. In summary, in vivo BMDH generation is a process in which the hematopoietic cell nucleus changes its identity and acquires hepatic features. These BMDHs have their own cell identity characterized by an expression pattern different from hematopoietic cells or hepatocytes. The role of these BMDHs in the liver requires further investigation.Oscar Quintana-BustamanteEsther GruesoRamon Garcia-EscuderoElvira ArzaAlberto Alvarez-BarrientosIsabel FabregatMaria Garcia-BravoNestor W MezaJose C SegoviaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33945 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Oscar Quintana-Bustamante Esther Grueso Ramon Garcia-Escudero Elvira Arza Alberto Alvarez-Barrientos Isabel Fabregat Maria Garcia-Bravo Nestor W Meza Jose C Segovia Cell fusion reprogramming leads to a specific hepatic expression pattern during mouse bone marrow derived hepatocyte formation in vivo. |
| description |
The fusion of bone marrow (BM) hematopoietic cells with hepatocytes to generate BM derived hepatocytes (BMDH) is a natural process, which is enhanced in damaged tissues. However, the reprogramming needed to generate BMDH and the identity of the resultant cells is essentially unknown. In a mouse model of chronic liver damage, here we identify a modification in the chromatin structure of the hematopoietic nucleus during BMDH formation, accompanied by the loss of the key hematopoietic transcription factor PU.1/Sfpi1 (SFFV proviral integration 1) and gain of the key hepatic transcriptional regulator HNF-1A homeobox A (HNF-1A/Hnf1a). Through genome-wide expression analysis of laser captured BMDH, a differential gene expression pattern was detected and the chromatin changes observed were confirmed at the level of chromatin regulator genes. Similarly, Tranforming Growth Factor-β1 (TGF-β(1)) and neurotransmitter (e.g. Prostaglandin E Receptor 4 [Ptger4]) pathway genes were over-expressed. In summary, in vivo BMDH generation is a process in which the hematopoietic cell nucleus changes its identity and acquires hepatic features. These BMDHs have their own cell identity characterized by an expression pattern different from hematopoietic cells or hepatocytes. The role of these BMDHs in the liver requires further investigation. |
| format |
article |
| author |
Oscar Quintana-Bustamante Esther Grueso Ramon Garcia-Escudero Elvira Arza Alberto Alvarez-Barrientos Isabel Fabregat Maria Garcia-Bravo Nestor W Meza Jose C Segovia |
| author_facet |
Oscar Quintana-Bustamante Esther Grueso Ramon Garcia-Escudero Elvira Arza Alberto Alvarez-Barrientos Isabel Fabregat Maria Garcia-Bravo Nestor W Meza Jose C Segovia |
| author_sort |
Oscar Quintana-Bustamante |
| title |
Cell fusion reprogramming leads to a specific hepatic expression pattern during mouse bone marrow derived hepatocyte formation in vivo. |
| title_short |
Cell fusion reprogramming leads to a specific hepatic expression pattern during mouse bone marrow derived hepatocyte formation in vivo. |
| title_full |
Cell fusion reprogramming leads to a specific hepatic expression pattern during mouse bone marrow derived hepatocyte formation in vivo. |
| title_fullStr |
Cell fusion reprogramming leads to a specific hepatic expression pattern during mouse bone marrow derived hepatocyte formation in vivo. |
| title_full_unstemmed |
Cell fusion reprogramming leads to a specific hepatic expression pattern during mouse bone marrow derived hepatocyte formation in vivo. |
| title_sort |
cell fusion reprogramming leads to a specific hepatic expression pattern during mouse bone marrow derived hepatocyte formation in vivo. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/7e5ee3b5815344a4ae5686ff3487d995 |
| work_keys_str_mv |
AT oscarquintanabustamante cellfusionreprogrammingleadstoaspecifichepaticexpressionpatternduringmousebonemarrowderivedhepatocyteformationinvivo AT esthergrueso cellfusionreprogrammingleadstoaspecifichepaticexpressionpatternduringmousebonemarrowderivedhepatocyteformationinvivo AT ramongarciaescudero cellfusionreprogrammingleadstoaspecifichepaticexpressionpatternduringmousebonemarrowderivedhepatocyteformationinvivo AT elviraarza cellfusionreprogrammingleadstoaspecifichepaticexpressionpatternduringmousebonemarrowderivedhepatocyteformationinvivo AT albertoalvarezbarrientos cellfusionreprogrammingleadstoaspecifichepaticexpressionpatternduringmousebonemarrowderivedhepatocyteformationinvivo AT isabelfabregat cellfusionreprogrammingleadstoaspecifichepaticexpressionpatternduringmousebonemarrowderivedhepatocyteformationinvivo AT mariagarciabravo cellfusionreprogrammingleadstoaspecifichepaticexpressionpatternduringmousebonemarrowderivedhepatocyteformationinvivo AT nestorwmeza cellfusionreprogrammingleadstoaspecifichepaticexpressionpatternduringmousebonemarrowderivedhepatocyteformationinvivo AT josecsegovia cellfusionreprogrammingleadstoaspecifichepaticexpressionpatternduringmousebonemarrowderivedhepatocyteformationinvivo |
| _version_ |
1718423484134064128 |