Components of the canonical and non-canonical Wnt pathways are not mis-expressed in pituitary tumors.

<h4>Introduction</h4>Canonical and non-canonical Wnt pathways are involved in the genesis of multiple tumors; however, their role in pituitary tumorigenesis is mostly unknown.<h4>Objective</h4>This study evaluated gene and protein expression of Wnt pathways in pituitary tumor...

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Autores principales:	Leandro Machado Colli, Fabiano Saggioro, Luciano Neder Serafini, Renata Costa Camargo, Helio Rubens Machado, Ayrton Custodio Moreira, Sonir R Antonini, Margaret de Castro
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e63f29dbf354849b93683841d01d227
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spelling	oai:doaj.org-article:7e63f29dbf354849b93683841d01d2272021-11-18T07:47:44ZComponents of the canonical and non-canonical Wnt pathways are not mis-expressed in pituitary tumors.1932-620310.1371/journal.pone.0062424https://doaj.org/article/7e63f29dbf354849b93683841d01d2272013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23638078/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Introduction</h4>Canonical and non-canonical Wnt pathways are involved in the genesis of multiple tumors; however, their role in pituitary tumorigenesis is mostly unknown.<h4>Objective</h4>This study evaluated gene and protein expression of Wnt pathways in pituitary tumors and whether these expression correlate to clinical outcome.<h4>Materials and methods</h4>Genes of the WNT canonical pathway: activating ligands (WNT11, WNT4, WNT5A), binding inhibitors (DKK3, sFRP1), β-catenin (CTNNB1), β-catenin degradation complex (APC, AXIN1, GSK3β), inhibitor of β-catenin degradation complex (AKT1), sequester of β-catenin (CDH1), pathway effectors (TCF7, MAPK8, NFAT5), pathway mediators (DVL-1, DVL-2, DVL-3, PRICKLE, VANGL1), target genes (MYB, MYC, WISP2, SPRY1, TP53, CCND1); calcium dependent pathway (PLCB1, CAMK2A, PRKCA, CHP); and planar cell polarity pathway (PTK7, DAAM1, RHOA) were evaluated by QPCR, in 19 GH-, 18 ACTH-secreting, 21 non-secreting (NS) pituitary tumors, and 5 normal pituitaries. Also, the main effectors of canonical (β-catenin), planar cell polarity (JNK), and calcium dependent (NFAT5) Wnt pathways were evaluated by immunohistochemistry.<h4>Results</h4>There are no differences in gene expression of canonical and non-canonical Wnt pathways between all studied subtypes of pituitary tumors and normal pituitaries, except for WISP2, which was over-expressed in ACTH-secreting tumors compared to normal pituitaries (4.8x; p = 0.02), NS pituitary tumors (7.7x; p = 0.004) and GH-secreting tumors (5.0x; p = 0.05). β-catenin, NFAT5 and JNK proteins showed no expression in normal pituitaries and in any of the pituitary tumor subtypes. Furthermore, no association of the studied gene or protein expression was observed with tumor size, recurrence, and progressive disease. The hierarchical clustering showed a regular pattern of genes of the canonical and non-canonical Wnt pathways randomly distributed throughout the dendrogram.<h4>Conclusions</h4>Our data reinforce previous reports suggesting no activation of canonical Wnt pathway in pituitary tumorigenesis. Moreover, we describe, for the first time, evidence that non-canonical Wnt pathways are also not mis-expressed in the pituitary tumors.Leandro Machado ColliFabiano SaggioroLuciano Neder SerafiniRenata Costa CamargoHelio Rubens MachadoAyrton Custodio MoreiraSonir R AntoniniMargaret de CastroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62424 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leandro Machado Colli Fabiano Saggioro Luciano Neder Serafini Renata Costa Camargo Helio Rubens Machado Ayrton Custodio Moreira Sonir R Antonini Margaret de Castro Components of the canonical and non-canonical Wnt pathways are not mis-expressed in pituitary tumors.
description	<h4>Introduction</h4>Canonical and non-canonical Wnt pathways are involved in the genesis of multiple tumors; however, their role in pituitary tumorigenesis is mostly unknown.<h4>Objective</h4>This study evaluated gene and protein expression of Wnt pathways in pituitary tumors and whether these expression correlate to clinical outcome.<h4>Materials and methods</h4>Genes of the WNT canonical pathway: activating ligands (WNT11, WNT4, WNT5A), binding inhibitors (DKK3, sFRP1), β-catenin (CTNNB1), β-catenin degradation complex (APC, AXIN1, GSK3β), inhibitor of β-catenin degradation complex (AKT1), sequester of β-catenin (CDH1), pathway effectors (TCF7, MAPK8, NFAT5), pathway mediators (DVL-1, DVL-2, DVL-3, PRICKLE, VANGL1), target genes (MYB, MYC, WISP2, SPRY1, TP53, CCND1); calcium dependent pathway (PLCB1, CAMK2A, PRKCA, CHP); and planar cell polarity pathway (PTK7, DAAM1, RHOA) were evaluated by QPCR, in 19 GH-, 18 ACTH-secreting, 21 non-secreting (NS) pituitary tumors, and 5 normal pituitaries. Also, the main effectors of canonical (β-catenin), planar cell polarity (JNK), and calcium dependent (NFAT5) Wnt pathways were evaluated by immunohistochemistry.<h4>Results</h4>There are no differences in gene expression of canonical and non-canonical Wnt pathways between all studied subtypes of pituitary tumors and normal pituitaries, except for WISP2, which was over-expressed in ACTH-secreting tumors compared to normal pituitaries (4.8x; p = 0.02), NS pituitary tumors (7.7x; p = 0.004) and GH-secreting tumors (5.0x; p = 0.05). β-catenin, NFAT5 and JNK proteins showed no expression in normal pituitaries and in any of the pituitary tumor subtypes. Furthermore, no association of the studied gene or protein expression was observed with tumor size, recurrence, and progressive disease. The hierarchical clustering showed a regular pattern of genes of the canonical and non-canonical Wnt pathways randomly distributed throughout the dendrogram.<h4>Conclusions</h4>Our data reinforce previous reports suggesting no activation of canonical Wnt pathway in pituitary tumorigenesis. Moreover, we describe, for the first time, evidence that non-canonical Wnt pathways are also not mis-expressed in the pituitary tumors.
format	article
author	Leandro Machado Colli Fabiano Saggioro Luciano Neder Serafini Renata Costa Camargo Helio Rubens Machado Ayrton Custodio Moreira Sonir R Antonini Margaret de Castro
author_facet	Leandro Machado Colli Fabiano Saggioro Luciano Neder Serafini Renata Costa Camargo Helio Rubens Machado Ayrton Custodio Moreira Sonir R Antonini Margaret de Castro
author_sort	Leandro Machado Colli
title	Components of the canonical and non-canonical Wnt pathways are not mis-expressed in pituitary tumors.
title_short	Components of the canonical and non-canonical Wnt pathways are not mis-expressed in pituitary tumors.
title_full	Components of the canonical and non-canonical Wnt pathways are not mis-expressed in pituitary tumors.
title_fullStr	Components of the canonical and non-canonical Wnt pathways are not mis-expressed in pituitary tumors.
title_full_unstemmed	Components of the canonical and non-canonical Wnt pathways are not mis-expressed in pituitary tumors.
title_sort	components of the canonical and non-canonical wnt pathways are not mis-expressed in pituitary tumors.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7e63f29dbf354849b93683841d01d227
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Components of the canonical and non-canonical Wnt pathways are not mis-expressed in pituitary tumors.

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