PLK1 protects against sepsis-induced intestinal barrier dysfunction

Abstract Sepsis and sepsis-associated intestinal barrier dysfunction are common in intensive care units, with high mortality. The aim of this study is to investigate whether Polo-like kinase 1 (PLK1) ameliorates sepsis-induced intestinal barrier dysfunction in the intestinal epithelium. The mouse in...

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Autores principales:	Yingya Cao, Qun Chen, Zhen Wang, Tao Yu, Jingyi Wu, Xiaogan Jiang, Xiaoju Jin, Weihua Lu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e63f768a0bb4f0a9fb1b82146717c3a
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spelling	oai:doaj.org-article:7e63f768a0bb4f0a9fb1b82146717c3a2021-12-02T15:07:46ZPLK1 protects against sepsis-induced intestinal barrier dysfunction10.1038/s41598-018-19573-x2045-2322https://doaj.org/article/7e63f768a0bb4f0a9fb1b82146717c3a2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19573-xhttps://doaj.org/toc/2045-2322Abstract Sepsis and sepsis-associated intestinal barrier dysfunction are common in intensive care units, with high mortality. The aim of this study is to investigate whether Polo-like kinase 1 (PLK1) ameliorates sepsis-induced intestinal barrier dysfunction in the intestinal epithelium. The mouse intestinal barrier was disrupted after Lipopolysaccharide (LPS) injection due to intestinal epithelial cell apoptosis and proliferation inhibition, accompanied by decreased PLK1. In HT-29 intestinal epithelial cells, LPS stimulation induced cell apoptosis and inhibited cell proliferation. Overexpression of PLK1 partly rescued the apoptosis and proliferation inhibition in HT29 cells caused by LPS. Finally, LPS stimulation promoted the reduction of PLK1, resulting in apoptosis and proliferation inhibition in intestinal epithelial cells, disrupting the intestinal epithelial barrier. These findings indicate that PLK1 might be a potential therapeutic target for the treatment of sepsis-induced intestinal barrier dysfunction.Yingya CaoQun ChenZhen WangTao YuJingyi WuXiaogan JiangXiaoju JinWeihua LuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yingya Cao Qun Chen Zhen Wang Tao Yu Jingyi Wu Xiaogan Jiang Xiaoju Jin Weihua Lu PLK1 protects against sepsis-induced intestinal barrier dysfunction
description	Abstract Sepsis and sepsis-associated intestinal barrier dysfunction are common in intensive care units, with high mortality. The aim of this study is to investigate whether Polo-like kinase 1 (PLK1) ameliorates sepsis-induced intestinal barrier dysfunction in the intestinal epithelium. The mouse intestinal barrier was disrupted after Lipopolysaccharide (LPS) injection due to intestinal epithelial cell apoptosis and proliferation inhibition, accompanied by decreased PLK1. In HT-29 intestinal epithelial cells, LPS stimulation induced cell apoptosis and inhibited cell proliferation. Overexpression of PLK1 partly rescued the apoptosis and proliferation inhibition in HT29 cells caused by LPS. Finally, LPS stimulation promoted the reduction of PLK1, resulting in apoptosis and proliferation inhibition in intestinal epithelial cells, disrupting the intestinal epithelial barrier. These findings indicate that PLK1 might be a potential therapeutic target for the treatment of sepsis-induced intestinal barrier dysfunction.
format	article
author	Yingya Cao Qun Chen Zhen Wang Tao Yu Jingyi Wu Xiaogan Jiang Xiaoju Jin Weihua Lu
author_facet	Yingya Cao Qun Chen Zhen Wang Tao Yu Jingyi Wu Xiaogan Jiang Xiaoju Jin Weihua Lu
author_sort	Yingya Cao
title	PLK1 protects against sepsis-induced intestinal barrier dysfunction
title_short	PLK1 protects against sepsis-induced intestinal barrier dysfunction
title_full	PLK1 protects against sepsis-induced intestinal barrier dysfunction
title_fullStr	PLK1 protects against sepsis-induced intestinal barrier dysfunction
title_full_unstemmed	PLK1 protects against sepsis-induced intestinal barrier dysfunction
title_sort	plk1 protects against sepsis-induced intestinal barrier dysfunction
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7e63f768a0bb4f0a9fb1b82146717c3a
work_keys_str_mv	AT yingyacao plk1protectsagainstsepsisinducedintestinalbarrierdysfunction AT qunchen plk1protectsagainstsepsisinducedintestinalbarrierdysfunction AT zhenwang plk1protectsagainstsepsisinducedintestinalbarrierdysfunction AT taoyu plk1protectsagainstsepsisinducedintestinalbarrierdysfunction AT jingyiwu plk1protectsagainstsepsisinducedintestinalbarrierdysfunction AT xiaoganjiang plk1protectsagainstsepsisinducedintestinalbarrierdysfunction AT xiaojujin plk1protectsagainstsepsisinducedintestinalbarrierdysfunction AT weihualu plk1protectsagainstsepsisinducedintestinalbarrierdysfunction
_version_	1718388397729382400

PLK1 protects against sepsis-induced intestinal barrier dysfunction

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