PROTECTIVE EFFECT OF LIGNOCAINE AGAINST ACETYLCHOLINE AND BRADYKININ INDUCED TRACHEAL TISSUE CONTRACTION

Objective: To evaluate the protective effect of lignocaine against acetylcholine and bradykinin induced airway contraction of isolated tracheal tissue of guinea pig in vitro. Study Design: Laboratory based quasi experimental study. Place and Duration of Study: Pharmacology department in collab...

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Autores principales:	Fatima Qasim Malik, Bushra Tayyaba Khan, Mahjabeen Sharif
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2019
Materias:	acetylcholine bradykinin isolated forced transducer isolated trachea Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7e65fb39b9aa4936a4a8c76c11d081c4
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spelling	oai:doaj.org-article:7e65fb39b9aa4936a4a8c76c11d081c42021-11-08T02:36:17ZPROTECTIVE EFFECT OF LIGNOCAINE AGAINST ACETYLCHOLINE AND BRADYKININ INDUCED TRACHEAL TISSUE CONTRACTION0030-96482411-8842https://doaj.org/article/7e65fb39b9aa4936a4a8c76c11d081c42019-12-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/3639/2460https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To evaluate the protective effect of lignocaine against acetylcholine and bradykinin induced airway contraction of isolated tracheal tissue of guinea pig in vitro. Study Design: Laboratory based quasi experimental study. Place and Duration of Study: Pharmacology department in collaboration with Physiology Department at Army Medical College, Rawalpindi, from Jan 2016 to Oct 2016. Methodology: Effects of cumulative doses of acetylcholine (10-6-10-3 M) and bradykinin (11μg -66 μg) in the presence of fixed dose of lignocaine (1mM) were observed on isolated tracheal smooth muscle of guinea pig by constructing cumulative dose response curves. Isometric Force Transducer DT-475 (USA) attached to Power Lab data Acquisition Unit, was used to record the tracheal smooth muscle contractions. Results: Acetylcholine and bradykinin reversibly increased the tracheal muscle contractions of guinea pig. Maximum amplitude of contraction with acetylcholine and bradykinin alone & acetylcholine and bradykinin pretreated with lignocaine were 0.025 ± 0.0009 mV, 0.013 ± 0.0007 mV, 0.013 ± 0.0012 mV and 0.007 ± 0.0002 mV respectively. So lignocaine significantly ameliorated acetylcholine and bradykinin induced contraction. Conclusion: Lignocaine significantly inhibited the constrictor response of acetylcholine and bradykinin. The percent inhibition was more for acetylcholine than for bradykinin induced tracheal tissue contraction. So it is suggested that lignocaine may be used as spinal anesthesia in asthmatic patients undergoing surgeries due to its bronchodilatory effects.Fatima Qasim MalikBushra Tayyaba KhanMahjabeen SharifArmy Medical College Rawalpindiarticleacetylcholinebradykininisolated forced transducerisolated tracheaMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 69, Iss 6, Pp 1221-1226 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	acetylcholine bradykinin isolated forced transducer isolated trachea Medicine R Medicine (General) R5-920
spellingShingle	acetylcholine bradykinin isolated forced transducer isolated trachea Medicine R Medicine (General) R5-920 Fatima Qasim Malik Bushra Tayyaba Khan Mahjabeen Sharif PROTECTIVE EFFECT OF LIGNOCAINE AGAINST ACETYLCHOLINE AND BRADYKININ INDUCED TRACHEAL TISSUE CONTRACTION
description	Objective: To evaluate the protective effect of lignocaine against acetylcholine and bradykinin induced airway contraction of isolated tracheal tissue of guinea pig in vitro. Study Design: Laboratory based quasi experimental study. Place and Duration of Study: Pharmacology department in collaboration with Physiology Department at Army Medical College, Rawalpindi, from Jan 2016 to Oct 2016. Methodology: Effects of cumulative doses of acetylcholine (10-6-10-3 M) and bradykinin (11μg -66 μg) in the presence of fixed dose of lignocaine (1mM) were observed on isolated tracheal smooth muscle of guinea pig by constructing cumulative dose response curves. Isometric Force Transducer DT-475 (USA) attached to Power Lab data Acquisition Unit, was used to record the tracheal smooth muscle contractions. Results: Acetylcholine and bradykinin reversibly increased the tracheal muscle contractions of guinea pig. Maximum amplitude of contraction with acetylcholine and bradykinin alone & acetylcholine and bradykinin pretreated with lignocaine were 0.025 ± 0.0009 mV, 0.013 ± 0.0007 mV, 0.013 ± 0.0012 mV and 0.007 ± 0.0002 mV respectively. So lignocaine significantly ameliorated acetylcholine and bradykinin induced contraction. Conclusion: Lignocaine significantly inhibited the constrictor response of acetylcholine and bradykinin. The percent inhibition was more for acetylcholine than for bradykinin induced tracheal tissue contraction. So it is suggested that lignocaine may be used as spinal anesthesia in asthmatic patients undergoing surgeries due to its bronchodilatory effects.
format	article
author	Fatima Qasim Malik Bushra Tayyaba Khan Mahjabeen Sharif
author_facet	Fatima Qasim Malik Bushra Tayyaba Khan Mahjabeen Sharif
author_sort	Fatima Qasim Malik
title	PROTECTIVE EFFECT OF LIGNOCAINE AGAINST ACETYLCHOLINE AND BRADYKININ INDUCED TRACHEAL TISSUE CONTRACTION
title_short	PROTECTIVE EFFECT OF LIGNOCAINE AGAINST ACETYLCHOLINE AND BRADYKININ INDUCED TRACHEAL TISSUE CONTRACTION
title_full	PROTECTIVE EFFECT OF LIGNOCAINE AGAINST ACETYLCHOLINE AND BRADYKININ INDUCED TRACHEAL TISSUE CONTRACTION
title_fullStr	PROTECTIVE EFFECT OF LIGNOCAINE AGAINST ACETYLCHOLINE AND BRADYKININ INDUCED TRACHEAL TISSUE CONTRACTION
title_full_unstemmed	PROTECTIVE EFFECT OF LIGNOCAINE AGAINST ACETYLCHOLINE AND BRADYKININ INDUCED TRACHEAL TISSUE CONTRACTION
title_sort	protective effect of lignocaine against acetylcholine and bradykinin induced tracheal tissue contraction
publisher	Army Medical College Rawalpindi
publishDate	2019
url	https://doaj.org/article/7e65fb39b9aa4936a4a8c76c11d081c4
work_keys_str_mv	AT fatimaqasimmalik protectiveeffectoflignocaineagainstacetylcholineandbradykinininducedtrachealtissuecontraction AT bushratayyabakhan protectiveeffectoflignocaineagainstacetylcholineandbradykinininducedtrachealtissuecontraction AT mahjabeensharif protectiveeffectoflignocaineagainstacetylcholineandbradykinininducedtrachealtissuecontraction
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PROTECTIVE EFFECT OF LIGNOCAINE AGAINST ACETYLCHOLINE AND BRADYKININ INDUCED TRACHEAL TISSUE CONTRACTION

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