Vitamin D Pretreatment Attenuates Ciprofloxacin-Induced Antibacterial Activity

Majed M Masadeh,1 Karem H Alzoubi,2 Bashar M Al-Taani,1 Majd M Masadeh,1 Zainah O Aburashed,1,2 Nasr Alrabadi3 1Department of Pharmaceutical Technology, Jordan University of Science and Technology, Irbid 22110, Jordan; 2Department of Clinical Pharmacy, Jordan University of Science and Technology, Ir...

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Autores principales:	Masadeh MM, Alzoubi KH, Al-Taani BM, Aburashed ZO, Alrabadi N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	fluoroquinolones vitamins mic zones of inhibition antimicrobial susceptibility oxidative stress. Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/7e69d6da2ecd4595a7a9d6bec836ba62
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spelling	oai:doaj.org-article:7e69d6da2ecd4595a7a9d6bec836ba622021-12-02T11:15:42ZVitamin D Pretreatment Attenuates Ciprofloxacin-Induced Antibacterial Activity1179-1438https://doaj.org/article/7e69d6da2ecd4595a7a9d6bec836ba622020-10-01T00:00:00Zhttps://www.dovepress.com/vitamin-d-pretreatment-attenuates-ciprofloxacin-induced-antibacterial--peer-reviewed-article-CPAAhttps://doaj.org/toc/1179-1438Majed M Masadeh,1 Karem H Alzoubi,2 Bashar M Al-Taani,1 Majd M Masadeh,1 Zainah O Aburashed,1,2 Nasr Alrabadi3 1Department of Pharmaceutical Technology, Jordan University of Science and Technology, Irbid 22110, Jordan; 2Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan; 3Department of Pharmacology, Faculty of Medicine, Jordan University of Science and Technology, Irbid 22110, JordanCorrespondence: Majed M MasadehDepartment of Pharmaceutical Technology, Jordan University of Science and Technology, Irbid 22110, JordanEmail mmmasadeh@just.edu.joBackground: Ciprofloxacin is an antimicrobial that is commonly used to treat several types of infections. It exerts its antimicrobial activity through interfering with bacterial DNA replication and transcription, leading to increase oxidative stress and eventually bacterial death. Vitamin D, on the other hand, has been found to have DNA protective and antioxidant effects. In the current study, the possible interactive effect of vitamin D on ciprofloxacin-induced cytotoxicity was investigated in various standard bacterial strains.Methods: The bacterial strains that were used include Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis, Acinetobacter baumannii, Proteus mirabilis, and Klebsiella pneumoniae. The antibacterial effect of ciprofloxacin with and without vitamin D treatment of the bacteria was assessed using disc diffusion method and by measuring the minimum inhibitory concentration (MIC) and zones of inhibition of bacterial growth. Moreover, reactive oxygen species (ROS) generation after pretreatment of E. Coli cells with ciprofloxacin and/or vitamin D was measured as a function of as a function of hydrogen peroxide generation.Results: Ciprofloxacin demonstrated a potent antibacterial effect against the tested strains of bacteria. Moreover, pretreatment with vitamin D resulted in protecting the bacteria from the cytotoxicity of ciprofloxacin, this was indicated by the significantly smaller zones of inhibition and higher MIC values compared to ciprofloxacin alone as well as reduced ciprofloxacin-induced ROS generation after treatment with vitamin D.Conclusion: Results revealed the possible reduction in the activity of ciprofloxacin when used in combination with vitamin D. This could be explained by the ability of vitamin D to reduce oxidative stress in the bacterial cells.Keywords: fluoroquinolones, vitamins, MIC, zones of inhibition, antimicrobial susceptibility, oxidative stressMasadeh MMAlzoubi KHAl-Taani BMMasadeh MMAburashed ZOAlrabadi NDove Medical Pressarticlefluoroquinolonesvitaminsmiczones of inhibitionantimicrobial susceptibilityoxidative stress.Therapeutics. PharmacologyRM1-950ENClinical Pharmacology: Advances and Applications, Vol Volume 12, Pp 171-176 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	fluoroquinolones vitamins mic zones of inhibition antimicrobial susceptibility oxidative stress. Therapeutics. Pharmacology RM1-950
spellingShingle	fluoroquinolones vitamins mic zones of inhibition antimicrobial susceptibility oxidative stress. Therapeutics. Pharmacology RM1-950 Masadeh MM Alzoubi KH Al-Taani BM Masadeh MM Aburashed ZO Alrabadi N Vitamin D Pretreatment Attenuates Ciprofloxacin-Induced Antibacterial Activity
description	Majed M Masadeh,1 Karem H Alzoubi,2 Bashar M Al-Taani,1 Majd M Masadeh,1 Zainah O Aburashed,1,2 Nasr Alrabadi3 1Department of Pharmaceutical Technology, Jordan University of Science and Technology, Irbid 22110, Jordan; 2Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan; 3Department of Pharmacology, Faculty of Medicine, Jordan University of Science and Technology, Irbid 22110, JordanCorrespondence: Majed M MasadehDepartment of Pharmaceutical Technology, Jordan University of Science and Technology, Irbid 22110, JordanEmail mmmasadeh@just.edu.joBackground: Ciprofloxacin is an antimicrobial that is commonly used to treat several types of infections. It exerts its antimicrobial activity through interfering with bacterial DNA replication and transcription, leading to increase oxidative stress and eventually bacterial death. Vitamin D, on the other hand, has been found to have DNA protective and antioxidant effects. In the current study, the possible interactive effect of vitamin D on ciprofloxacin-induced cytotoxicity was investigated in various standard bacterial strains.Methods: The bacterial strains that were used include Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis, Acinetobacter baumannii, Proteus mirabilis, and Klebsiella pneumoniae. The antibacterial effect of ciprofloxacin with and without vitamin D treatment of the bacteria was assessed using disc diffusion method and by measuring the minimum inhibitory concentration (MIC) and zones of inhibition of bacterial growth. Moreover, reactive oxygen species (ROS) generation after pretreatment of E. Coli cells with ciprofloxacin and/or vitamin D was measured as a function of as a function of hydrogen peroxide generation.Results: Ciprofloxacin demonstrated a potent antibacterial effect against the tested strains of bacteria. Moreover, pretreatment with vitamin D resulted in protecting the bacteria from the cytotoxicity of ciprofloxacin, this was indicated by the significantly smaller zones of inhibition and higher MIC values compared to ciprofloxacin alone as well as reduced ciprofloxacin-induced ROS generation after treatment with vitamin D.Conclusion: Results revealed the possible reduction in the activity of ciprofloxacin when used in combination with vitamin D. This could be explained by the ability of vitamin D to reduce oxidative stress in the bacterial cells.Keywords: fluoroquinolones, vitamins, MIC, zones of inhibition, antimicrobial susceptibility, oxidative stress
format	article
author	Masadeh MM Alzoubi KH Al-Taani BM Masadeh MM Aburashed ZO Alrabadi N
author_facet	Masadeh MM Alzoubi KH Al-Taani BM Masadeh MM Aburashed ZO Alrabadi N
author_sort	Masadeh MM
title	Vitamin D Pretreatment Attenuates Ciprofloxacin-Induced Antibacterial Activity
title_short	Vitamin D Pretreatment Attenuates Ciprofloxacin-Induced Antibacterial Activity
title_full	Vitamin D Pretreatment Attenuates Ciprofloxacin-Induced Antibacterial Activity
title_fullStr	Vitamin D Pretreatment Attenuates Ciprofloxacin-Induced Antibacterial Activity
title_full_unstemmed	Vitamin D Pretreatment Attenuates Ciprofloxacin-Induced Antibacterial Activity
title_sort	vitamin d pretreatment attenuates ciprofloxacin-induced antibacterial activity
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/7e69d6da2ecd4595a7a9d6bec836ba62
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Vitamin D Pretreatment Attenuates Ciprofloxacin-Induced Antibacterial Activity

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