Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection

Lavender species are widely distributed in their wild forms around the Mediterranean Basin and they are also cultivated worldwide as improved and registered clonal varieties. The economic interest of the species belonging to the <i>Lavandula</i> genus is determined by their use as orname...

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Autores principales:	Francesco Scariolo, Fabio Palumbo, Alessandro Vannozzi, Gio Batta Sacilotto, Marco Gazzola, Gianni Barcaccia
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Lavandula</i> NGS genotyping by RAD sequencing flavonoids terpenes chloroplast DNA barcoding Genetics QH426-470
Acceso en línea:	https://doaj.org/article/7e6d0cbf4fcd49719c37daa2e08d9635
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spelling	oai:doaj.org-article:7e6d0cbf4fcd49719c37daa2e08d96352021-11-25T17:40:18ZGenotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection10.3390/genes121116562073-4425https://doaj.org/article/7e6d0cbf4fcd49719c37daa2e08d96352021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1656https://doaj.org/toc/2073-4425Lavender species are widely distributed in their wild forms around the Mediterranean Basin and they are also cultivated worldwide as improved and registered clonal varieties. The economic interest of the species belonging to the <i>Lavandula</i> genus is determined by their use as ornamental plants and important source of essential oils that are destinated to the production of cosmetics, pharmaceuticals and foodstuffs. Because of the increasing number of cases of illegal commercialization of selected varieties, the protection of plant breeders’ rights has become of main relevance for the recognition of breeding companies’ royalties. With this aim, genomic tools based on molecular markers have been demonstrated to be very reliable and transferable among laboratories, and also much more informative than morphological descriptors. With the rising of the next-generation sequencing (NGS) technologies, several genotyping-by-sequencing approaches are now available. This study deals with a deep characterization of 15 varietal clones, belonging to two distinct <i>Lavandula</i> species, by means of restriction-site associated DNA sequencing (RAD-Seq). We demonstrated that this technology screens single nucleotide variants that enable to assess the genetic identity of individual accessions, to reconstruct genetic relationships among related breeding lines, to group them into genetically distinguishable main subclusters, and to assign their molecular lineages to distinct ancestors. Moreover, a number of polymorphic sites were identified within genes putatively involved in biosynthetic pathways related to both tissue pigmentation and terpene production, useful for breeding and/or protecting newly registered varieties. Overall, the results highlighted the presence of pure ancestries and interspecific hybrids for the analyzed <i>Lavandula</i> species, and demonstrated that RAD-Seq analysis is very informative and highly reliable for characterizing <i>Lavandula</i> clones and managing plant variety protection.Francesco ScarioloFabio PalumboAlessandro VannozziGio Batta SacilottoMarco GazzolaGianni BarcacciaMDPI AGarticle<i>Lavandula</i>NGSgenotyping by RAD sequencingflavonoidsterpeneschloroplast DNA barcodingGeneticsQH426-470ENGenes, Vol 12, Iss 1656, p 1656 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Lavandula</i> NGS genotyping by RAD sequencing flavonoids terpenes chloroplast DNA barcoding Genetics QH426-470
spellingShingle	<i>Lavandula</i> NGS genotyping by RAD sequencing flavonoids terpenes chloroplast DNA barcoding Genetics QH426-470 Francesco Scariolo Fabio Palumbo Alessandro Vannozzi Gio Batta Sacilotto Marco Gazzola Gianni Barcaccia Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection
description	Lavender species are widely distributed in their wild forms around the Mediterranean Basin and they are also cultivated worldwide as improved and registered clonal varieties. The economic interest of the species belonging to the <i>Lavandula</i> genus is determined by their use as ornamental plants and important source of essential oils that are destinated to the production of cosmetics, pharmaceuticals and foodstuffs. Because of the increasing number of cases of illegal commercialization of selected varieties, the protection of plant breeders’ rights has become of main relevance for the recognition of breeding companies’ royalties. With this aim, genomic tools based on molecular markers have been demonstrated to be very reliable and transferable among laboratories, and also much more informative than morphological descriptors. With the rising of the next-generation sequencing (NGS) technologies, several genotyping-by-sequencing approaches are now available. This study deals with a deep characterization of 15 varietal clones, belonging to two distinct <i>Lavandula</i> species, by means of restriction-site associated DNA sequencing (RAD-Seq). We demonstrated that this technology screens single nucleotide variants that enable to assess the genetic identity of individual accessions, to reconstruct genetic relationships among related breeding lines, to group them into genetically distinguishable main subclusters, and to assign their molecular lineages to distinct ancestors. Moreover, a number of polymorphic sites were identified within genes putatively involved in biosynthetic pathways related to both tissue pigmentation and terpene production, useful for breeding and/or protecting newly registered varieties. Overall, the results highlighted the presence of pure ancestries and interspecific hybrids for the analyzed <i>Lavandula</i> species, and demonstrated that RAD-Seq analysis is very informative and highly reliable for characterizing <i>Lavandula</i> clones and managing plant variety protection.
format	article
author	Francesco Scariolo Fabio Palumbo Alessandro Vannozzi Gio Batta Sacilotto Marco Gazzola Gianni Barcaccia
author_facet	Francesco Scariolo Fabio Palumbo Alessandro Vannozzi Gio Batta Sacilotto Marco Gazzola Gianni Barcaccia
author_sort	Francesco Scariolo
title	Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection
title_short	Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection
title_full	Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection
title_fullStr	Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection
title_full_unstemmed	Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection
title_sort	genotyping analysis by rad-seq reads is useful to assess the genetic identity and relationships of breeding lines in lavender species aimed at managing plant variety protection
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7e6d0cbf4fcd49719c37daa2e08d9635
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Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection

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