Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 prevents CCl4-induced liver cirrhosis by protecting the intestinal barrier in rats

Abstract Alterations in the gut microbiome have been reported in liver cirrhosis, and probiotic interventions are considered a potential treatment strategy. This study aimed to evaluate the effects and mechanisms of Lactobacillus salivarius LI01, Pediococcus pentosaceus LI05, Lactobacillus rhamnosus...

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Autores principales:	Ding Shi, Longxian Lv, Daiqiong Fang, Wenrui Wu, Chenxia Hu, Lichen Xu, Yanfei Chen, Jing Guo, Xinjun Hu, Ang Li, Feifei Guo, Jianzhong Ye, Yating Li, Dewi Andayani, Lanjuan Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e6fbd3bfa8444a1b04146f163c64354
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spelling	oai:doaj.org-article:7e6fbd3bfa8444a1b04146f163c643542021-12-02T16:06:25ZAdministration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 prevents CCl4-induced liver cirrhosis by protecting the intestinal barrier in rats10.1038/s41598-017-07091-12045-2322https://doaj.org/article/7e6fbd3bfa8444a1b04146f163c643542017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07091-1https://doaj.org/toc/2045-2322Abstract Alterations in the gut microbiome have been reported in liver cirrhosis, and probiotic interventions are considered a potential treatment strategy. This study aimed to evaluate the effects and mechanisms of Lactobacillus salivarius LI01, Pediococcus pentosaceus LI05, Lactobacillus rhamnosus GG, Clostridium butyricum MIYAIRI and Bacillus licheniformis Zhengchangsheng on CCl4-induced cirrhotic rats. Only administration of LI01 or LI05 prevented liver fibrosis and down-regulated the hepatic expression of profibrogenic genes. Serum endotoxins, bacterial translocations (BTs), and destruction of intestinal mucosal ultrastructure were reduced in rats treated with LI01 or LI05, indicating maintenance of the gut barrier as a mechanism; this was further confirmed by the reduction of not only hepatic inflammatory cytokines, such as TNF-α, IL-6, and IL-17A, but also hepatic TLR2, TLR4, TLR5 and TLR9. Metagenomic sequencing of 16S rRNA gene showed an increase in potential beneficial bacteria, such as Elusimicrobium and Prevotella, and a decrease in pathogenic bacteria, such as Escherichia. These alterations in gut microbiome were correlated with profibrogenic genes, gut barrier markers and inflammatory cytokines. In conclusion, L. salivarius LI01 and P. pentosaceus LI05 attenuated liver fibrosis by protecting the intestinal barrier and promoting microbiome health. These results suggest novel strategies for the prevention of liver cirrhosis.Ding ShiLongxian LvDaiqiong FangWenrui WuChenxia HuLichen XuYanfei ChenJing GuoXinjun HuAng LiFeifei GuoJianzhong YeYating LiDewi AndayaniLanjuan LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ding Shi Longxian Lv Daiqiong Fang Wenrui Wu Chenxia Hu Lichen Xu Yanfei Chen Jing Guo Xinjun Hu Ang Li Feifei Guo Jianzhong Ye Yating Li Dewi Andayani Lanjuan Li Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 prevents CCl4-induced liver cirrhosis by protecting the intestinal barrier in rats
description	Abstract Alterations in the gut microbiome have been reported in liver cirrhosis, and probiotic interventions are considered a potential treatment strategy. This study aimed to evaluate the effects and mechanisms of Lactobacillus salivarius LI01, Pediococcus pentosaceus LI05, Lactobacillus rhamnosus GG, Clostridium butyricum MIYAIRI and Bacillus licheniformis Zhengchangsheng on CCl4-induced cirrhotic rats. Only administration of LI01 or LI05 prevented liver fibrosis and down-regulated the hepatic expression of profibrogenic genes. Serum endotoxins, bacterial translocations (BTs), and destruction of intestinal mucosal ultrastructure were reduced in rats treated with LI01 or LI05, indicating maintenance of the gut barrier as a mechanism; this was further confirmed by the reduction of not only hepatic inflammatory cytokines, such as TNF-α, IL-6, and IL-17A, but also hepatic TLR2, TLR4, TLR5 and TLR9. Metagenomic sequencing of 16S rRNA gene showed an increase in potential beneficial bacteria, such as Elusimicrobium and Prevotella, and a decrease in pathogenic bacteria, such as Escherichia. These alterations in gut microbiome were correlated with profibrogenic genes, gut barrier markers and inflammatory cytokines. In conclusion, L. salivarius LI01 and P. pentosaceus LI05 attenuated liver fibrosis by protecting the intestinal barrier and promoting microbiome health. These results suggest novel strategies for the prevention of liver cirrhosis.
format	article
author	Ding Shi Longxian Lv Daiqiong Fang Wenrui Wu Chenxia Hu Lichen Xu Yanfei Chen Jing Guo Xinjun Hu Ang Li Feifei Guo Jianzhong Ye Yating Li Dewi Andayani Lanjuan Li
author_facet	Ding Shi Longxian Lv Daiqiong Fang Wenrui Wu Chenxia Hu Lichen Xu Yanfei Chen Jing Guo Xinjun Hu Ang Li Feifei Guo Jianzhong Ye Yating Li Dewi Andayani Lanjuan Li
author_sort	Ding Shi
title	Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 prevents CCl4-induced liver cirrhosis by protecting the intestinal barrier in rats
title_short	Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 prevents CCl4-induced liver cirrhosis by protecting the intestinal barrier in rats
title_full	Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 prevents CCl4-induced liver cirrhosis by protecting the intestinal barrier in rats
title_fullStr	Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 prevents CCl4-induced liver cirrhosis by protecting the intestinal barrier in rats
title_full_unstemmed	Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 prevents CCl4-induced liver cirrhosis by protecting the intestinal barrier in rats
title_sort	administration of lactobacillus salivarius li01 or pediococcus pentosaceus li05 prevents ccl4-induced liver cirrhosis by protecting the intestinal barrier in rats
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7e6fbd3bfa8444a1b04146f163c64354
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Administration of Lactobacillus salivarius LI01 or Pediococcus pentosaceus LI05 prevents CCl4-induced liver cirrhosis by protecting the intestinal barrier in rats

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