Characterization and plasma measurement of the WE-14 peptide in patients with pheochromocytoma.

Granins and their derived peptides are valuable circulating biological markers of neuroendocrine tumors. The aim of the present study was to investigate the tumoral chromogranin A (CgA)-derived peptide WE-14 and the potential advantage to combine plasma WE-14 detection with the EM66 assay and the ex...

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Autores principales:	Johann Guillemot, Marlène Guérin, Erwan Thouënnon, Maité Montéro-Hadjadje, Jérôme Leprince, Hervé Lefebvre, Marc Klein, Mihaela Muresan, Youssef Anouar, Laurent Yon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e7d05c62cbf455b9e45e8807e0ac1da
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spelling	oai:doaj.org-article:7e7d05c62cbf455b9e45e8807e0ac1da2021-11-18T08:32:56ZCharacterization and plasma measurement of the WE-14 peptide in patients with pheochromocytoma.1932-620310.1371/journal.pone.0088698https://doaj.org/article/7e7d05c62cbf455b9e45e8807e0ac1da2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24523932/?tool=EBIhttps://doaj.org/toc/1932-6203Granins and their derived peptides are valuable circulating biological markers of neuroendocrine tumors. The aim of the present study was to investigate the tumoral chromogranin A (CgA)-derived peptide WE-14 and the potential advantage to combine plasma WE-14 detection with the EM66 assay and the existing current CgA assay for the diagnosis of pheochromocytoma. Compared to healthy volunteers, plasma WE-14 levels were 5.4-fold higher in patients with pheochromocytoma, but returned to normal values after surgical resection of the tumor. Determination of plasma CgA and EM66 concentrations in the same group of patients revealed that the test assays for these markers had an overall 84% diagnostic sensitivity, which is identical to that determined for WE-14. However, we found that WE-14 measurement improved the diagnostic sensitivity when combined with the results of CgA or EM66 assays. By combining the results of the three assays, the sensitivity for the diagnosis of pheochromocytoma was increased to 95%. In fact, the combination of WE-14 with either CgA or EM66 test assays achieved 100% sensitivity for the diagnosis of paragangliomas and sporadic or malignant pheochromocytomas if taken separately to account for the heterogeneity of the tumor. These data indicate that WE-14 is produced in pheochromocytoma and secreted into the general circulation, and that elevated plasma WE-14 levels are correlated with the occurrence of this chromaffin cell tumor. In addition, in association with other biological markers, such as CgA and/or EM66, WE-14 measurement systematically improves the diagnostic sensitivity for pheochromocytoma. These findings support the notion that granin-processing products may represent complementary tools for the diagnosis of neuroendocrine tumors.Johann GuillemotMarlène GuérinErwan ThouënnonMaité Montéro-HadjadjeJérôme LeprinceHervé LefebvreMarc KleinMihaela MuresanYoussef AnouarLaurent YonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88698 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johann Guillemot Marlène Guérin Erwan Thouënnon Maité Montéro-Hadjadje Jérôme Leprince Hervé Lefebvre Marc Klein Mihaela Muresan Youssef Anouar Laurent Yon Characterization and plasma measurement of the WE-14 peptide in patients with pheochromocytoma.
description	Granins and their derived peptides are valuable circulating biological markers of neuroendocrine tumors. The aim of the present study was to investigate the tumoral chromogranin A (CgA)-derived peptide WE-14 and the potential advantage to combine plasma WE-14 detection with the EM66 assay and the existing current CgA assay for the diagnosis of pheochromocytoma. Compared to healthy volunteers, plasma WE-14 levels were 5.4-fold higher in patients with pheochromocytoma, but returned to normal values after surgical resection of the tumor. Determination of plasma CgA and EM66 concentrations in the same group of patients revealed that the test assays for these markers had an overall 84% diagnostic sensitivity, which is identical to that determined for WE-14. However, we found that WE-14 measurement improved the diagnostic sensitivity when combined with the results of CgA or EM66 assays. By combining the results of the three assays, the sensitivity for the diagnosis of pheochromocytoma was increased to 95%. In fact, the combination of WE-14 with either CgA or EM66 test assays achieved 100% sensitivity for the diagnosis of paragangliomas and sporadic or malignant pheochromocytomas if taken separately to account for the heterogeneity of the tumor. These data indicate that WE-14 is produced in pheochromocytoma and secreted into the general circulation, and that elevated plasma WE-14 levels are correlated with the occurrence of this chromaffin cell tumor. In addition, in association with other biological markers, such as CgA and/or EM66, WE-14 measurement systematically improves the diagnostic sensitivity for pheochromocytoma. These findings support the notion that granin-processing products may represent complementary tools for the diagnosis of neuroendocrine tumors.
format	article
author	Johann Guillemot Marlène Guérin Erwan Thouënnon Maité Montéro-Hadjadje Jérôme Leprince Hervé Lefebvre Marc Klein Mihaela Muresan Youssef Anouar Laurent Yon
author_facet	Johann Guillemot Marlène Guérin Erwan Thouënnon Maité Montéro-Hadjadje Jérôme Leprince Hervé Lefebvre Marc Klein Mihaela Muresan Youssef Anouar Laurent Yon
author_sort	Johann Guillemot
title	Characterization and plasma measurement of the WE-14 peptide in patients with pheochromocytoma.
title_short	Characterization and plasma measurement of the WE-14 peptide in patients with pheochromocytoma.
title_full	Characterization and plasma measurement of the WE-14 peptide in patients with pheochromocytoma.
title_fullStr	Characterization and plasma measurement of the WE-14 peptide in patients with pheochromocytoma.
title_full_unstemmed	Characterization and plasma measurement of the WE-14 peptide in patients with pheochromocytoma.
title_sort	characterization and plasma measurement of the we-14 peptide in patients with pheochromocytoma.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/7e7d05c62cbf455b9e45e8807e0ac1da
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Characterization and plasma measurement of the WE-14 peptide in patients with pheochromocytoma.

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