BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.

We cloned cDNA for the Bombyx mori DJ-1 protein (BmDJ-1) from the brains of larvae. BmDJ-1 is composed of 190 amino acids and encoded by 672 nucleotides. Northern blot analysis showed that BmDJ-1 is transcribed as a 756-bp mRNA and has one isoform. Reverse transcriptase (RT)-PCR experiments revealed...

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Autores principales:	Hiroko Tabunoki, Hiroaki Ode, Yutaka Banno, Susumu Katsuma, Toru Shimada, Kazuei Mita, Kimiko Yamamoto, Ryoichi Sato, Reiko Ishii-Nozawa, Jun-ichi Satoh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7e9622de8c4f4f3c86eccf0066f3fcbb
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spelling	oai:doaj.org-article:7e9622de8c4f4f3c86eccf0066f3fcbb2021-11-18T06:56:52ZBmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.1932-620310.1371/journal.pone.0017683https://doaj.org/article/7e9622de8c4f4f3c86eccf0066f3fcbb2011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21455296/?tool=EBIhttps://doaj.org/toc/1932-6203We cloned cDNA for the Bombyx mori DJ-1 protein (BmDJ-1) from the brains of larvae. BmDJ-1 is composed of 190 amino acids and encoded by 672 nucleotides. Northern blot analysis showed that BmDJ-1 is transcribed as a 756-bp mRNA and has one isoform. Reverse transcriptase (RT)-PCR experiments revealed that the BmDJ-1 was present in the brain, fatbody, Malpighian tubule, ovary and testis but present in only low amounts in the silkgland and hemocyte of day 4 fifth instar larvae. Immunological analysis demonstrated the presence of BmDJ-1 in the brain, midgut, fatbody, Malpighian tubule, testis and ovary from the larvae to the adult. We found that BmDJ-1 has a unique expression pattern through the fifth instar larval to adult developmental stage. We assessed the anti-oxidative function of BmDJ-1 using rotenone (ROT) in day 3 fifth instar larvae. Administration of ROT to day 3 fifth instar larvae, together with exogenous (BmNPV-BmDJ-1 infection for 4 days in advance) BmDJ-1, produced significantly lower 24-h mortality in BmDJ-1 groups than in the control. 2D-PAGE revealed an isoelectric point (pI) shift to an acidic form for BmDJ-1 in BmN4 cells upon ROT stimulus. Among the factors examined for their effects on expression level of BmDJ-1 in the hemolymph, nitric oxide (NO) concentration was identified based on dramatic developmental stage-dependent changes. Administration of isosorbide dinitrate (ISDN), which is an NO donor, to BmN4 cells produced increased expression of BmDJ-1 compared to the control. These results suggest that BmDJ-1 might control oxidative stress in the cell due to NO and serves as a development modulation factor in B. mori.Hiroko TabunokiHiroaki OdeYutaka BannoSusumu KatsumaToru ShimadaKazuei MitaKimiko YamamotoRyoichi SatoReiko Ishii-NozawaJun-ichi SatohPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17683 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroko Tabunoki Hiroaki Ode Yutaka Banno Susumu Katsuma Toru Shimada Kazuei Mita Kimiko Yamamoto Ryoichi Sato Reiko Ishii-Nozawa Jun-ichi Satoh BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.
description	We cloned cDNA for the Bombyx mori DJ-1 protein (BmDJ-1) from the brains of larvae. BmDJ-1 is composed of 190 amino acids and encoded by 672 nucleotides. Northern blot analysis showed that BmDJ-1 is transcribed as a 756-bp mRNA and has one isoform. Reverse transcriptase (RT)-PCR experiments revealed that the BmDJ-1 was present in the brain, fatbody, Malpighian tubule, ovary and testis but present in only low amounts in the silkgland and hemocyte of day 4 fifth instar larvae. Immunological analysis demonstrated the presence of BmDJ-1 in the brain, midgut, fatbody, Malpighian tubule, testis and ovary from the larvae to the adult. We found that BmDJ-1 has a unique expression pattern through the fifth instar larval to adult developmental stage. We assessed the anti-oxidative function of BmDJ-1 using rotenone (ROT) in day 3 fifth instar larvae. Administration of ROT to day 3 fifth instar larvae, together with exogenous (BmNPV-BmDJ-1 infection for 4 days in advance) BmDJ-1, produced significantly lower 24-h mortality in BmDJ-1 groups than in the control. 2D-PAGE revealed an isoelectric point (pI) shift to an acidic form for BmDJ-1 in BmN4 cells upon ROT stimulus. Among the factors examined for their effects on expression level of BmDJ-1 in the hemolymph, nitric oxide (NO) concentration was identified based on dramatic developmental stage-dependent changes. Administration of isosorbide dinitrate (ISDN), which is an NO donor, to BmN4 cells produced increased expression of BmDJ-1 compared to the control. These results suggest that BmDJ-1 might control oxidative stress in the cell due to NO and serves as a development modulation factor in B. mori.
format	article
author	Hiroko Tabunoki Hiroaki Ode Yutaka Banno Susumu Katsuma Toru Shimada Kazuei Mita Kimiko Yamamoto Ryoichi Sato Reiko Ishii-Nozawa Jun-ichi Satoh
author_facet	Hiroko Tabunoki Hiroaki Ode Yutaka Banno Susumu Katsuma Toru Shimada Kazuei Mita Kimiko Yamamoto Ryoichi Sato Reiko Ishii-Nozawa Jun-ichi Satoh
author_sort	Hiroko Tabunoki
title	BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.
title_short	BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.
title_full	BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.
title_fullStr	BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.
title_full_unstemmed	BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.
title_sort	bmdj-1 is a key regulator of oxidative modification in the development of the silkworm, bombyx mori.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/7e9622de8c4f4f3c86eccf0066f3fcbb
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BmDJ-1 is a key regulator of oxidative modification in the development of the silkworm, Bombyx mori.

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