Prolonged retention of liposomes in the pleural cavity of normal mice and high tumor distribution in mice with malignant pleural effusion, after intrapleural injection

A Marazioti,1 K Papadia,2 A Giannou,3 GT Stathopoulos,3,4 SG Antimisiaris1,21Foundation for Research and Technology Hellas, Institute of Chemical Engineering Sciences (FORTH/ICE-HT), Rio, Greece; 2Laboratory of Pharmaceutical Technology, Department of Pharmacy, University of Patras, Rio, Greece; 3La...

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Autores principales:	Marazioti A, Papadia K, Giannou A, Stathopoulos GT, Antimisiaris SG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Nanoparticles Sustained release Local administration Lung Cancer mesothelioma Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7e9870be6cc8483cb8bdfd9b194402f3
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spelling	oai:doaj.org-article:7e9870be6cc8483cb8bdfd9b194402f32021-12-02T03:21:08ZProlonged retention of liposomes in the pleural cavity of normal mice and high tumor distribution in mice with malignant pleural effusion, after intrapleural injection1178-2013https://doaj.org/article/7e9870be6cc8483cb8bdfd9b194402f32019-05-01T00:00:00Zhttps://www.dovepress.com/prolonged-retention-of-liposomes-in-the-pleural-cavity-of-normal-mice--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013A Marazioti,1 K Papadia,2 A Giannou,3 GT Stathopoulos,3,4 SG Antimisiaris1,21Foundation for Research and Technology Hellas, Institute of Chemical Engineering Sciences (FORTH/ICE-HT), Rio, Greece; 2Laboratory of Pharmaceutical Technology, Department of Pharmacy, University of Patras, Rio, Greece; 3Laboratory for Molecular Respiratory Carcinogenesis, Department of Physiology, Faculty of Medicine, University of Patras, Rio Greece; 4Comprehensive Pneumonology Center and Institute for Lung Biology and Disease, University Hospital, Ludwig-Maximilians University and Helmholtz Center Munich, Munich, GermanyBackground: Intrapleural administration of compounds is a lung targeted, innovative therapeutic strategy for mesothelioma, which can be refined as a route for drug delivery that minimizes the potential for systemic toxicity. However, little is currently known about the retention of liposomal drugs at the site, after such topical administration.Purpose: To evaluate the retention of liposomes in lungs following intrapleural injection, and how this might be modulated by liposome properties and disease progression.Methods: DiR-incorporating liposomes with various lipid compositions and sizes were prepared, characterized (for size distribution and zeta potential) and injected intrapleurally in normal mice and mice with malignant pleural effusion (MPE). DiR retention in pleural cavity was followed by biofluorescence imaging.Results: Experimental results demonstrate that liposome size and PEG-coating, have a significant effect on residence time in the pleural cavity; negative surface charge does not. More than 20% liposomal-DiR is retained 24 d post-injection (in some cases), indicating the high potential towards localized diseases. Ex-vivo liposomal-DiR signal in tumors of MPE mice was similar to signal in liver, suggesting high tumor targeting potential of intrapleurally injected liposomes. Finally, no difference was noticed in liposomal-DiR retention between tumor-inoculated (MPE) and healthy mice, indicating the stability of liposomes in the presence of effusion (in MPE mice).Conclusion: The current study provides novel insights for using liposomes by intrapleural administration for the treatment of lung diseases.Keywords: nanoparticles, sustained release, local administration, lung, cancer, mesotheliomaMarazioti APapadia KGiannou AStathopoulos GTAntimisiaris SGDove Medical PressarticleNanoparticlesSustained releaseLocal administrationLungCancermesotheliomaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3773-3784 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanoparticles Sustained release Local administration Lung Cancer mesothelioma Medicine (General) R5-920
spellingShingle	Nanoparticles Sustained release Local administration Lung Cancer mesothelioma Medicine (General) R5-920 Marazioti A Papadia K Giannou A Stathopoulos GT Antimisiaris SG Prolonged retention of liposomes in the pleural cavity of normal mice and high tumor distribution in mice with malignant pleural effusion, after intrapleural injection
description	A Marazioti,1 K Papadia,2 A Giannou,3 GT Stathopoulos,3,4 SG Antimisiaris1,21Foundation for Research and Technology Hellas, Institute of Chemical Engineering Sciences (FORTH/ICE-HT), Rio, Greece; 2Laboratory of Pharmaceutical Technology, Department of Pharmacy, University of Patras, Rio, Greece; 3Laboratory for Molecular Respiratory Carcinogenesis, Department of Physiology, Faculty of Medicine, University of Patras, Rio Greece; 4Comprehensive Pneumonology Center and Institute for Lung Biology and Disease, University Hospital, Ludwig-Maximilians University and Helmholtz Center Munich, Munich, GermanyBackground: Intrapleural administration of compounds is a lung targeted, innovative therapeutic strategy for mesothelioma, which can be refined as a route for drug delivery that minimizes the potential for systemic toxicity. However, little is currently known about the retention of liposomal drugs at the site, after such topical administration.Purpose: To evaluate the retention of liposomes in lungs following intrapleural injection, and how this might be modulated by liposome properties and disease progression.Methods: DiR-incorporating liposomes with various lipid compositions and sizes were prepared, characterized (for size distribution and zeta potential) and injected intrapleurally in normal mice and mice with malignant pleural effusion (MPE). DiR retention in pleural cavity was followed by biofluorescence imaging.Results: Experimental results demonstrate that liposome size and PEG-coating, have a significant effect on residence time in the pleural cavity; negative surface charge does not. More than 20% liposomal-DiR is retained 24 d post-injection (in some cases), indicating the high potential towards localized diseases. Ex-vivo liposomal-DiR signal in tumors of MPE mice was similar to signal in liver, suggesting high tumor targeting potential of intrapleurally injected liposomes. Finally, no difference was noticed in liposomal-DiR retention between tumor-inoculated (MPE) and healthy mice, indicating the stability of liposomes in the presence of effusion (in MPE mice).Conclusion: The current study provides novel insights for using liposomes by intrapleural administration for the treatment of lung diseases.Keywords: nanoparticles, sustained release, local administration, lung, cancer, mesothelioma
format	article
author	Marazioti A Papadia K Giannou A Stathopoulos GT Antimisiaris SG
author_facet	Marazioti A Papadia K Giannou A Stathopoulos GT Antimisiaris SG
author_sort	Marazioti A
title	Prolonged retention of liposomes in the pleural cavity of normal mice and high tumor distribution in mice with malignant pleural effusion, after intrapleural injection
title_short	Prolonged retention of liposomes in the pleural cavity of normal mice and high tumor distribution in mice with malignant pleural effusion, after intrapleural injection
title_full	Prolonged retention of liposomes in the pleural cavity of normal mice and high tumor distribution in mice with malignant pleural effusion, after intrapleural injection
title_fullStr	Prolonged retention of liposomes in the pleural cavity of normal mice and high tumor distribution in mice with malignant pleural effusion, after intrapleural injection
title_full_unstemmed	Prolonged retention of liposomes in the pleural cavity of normal mice and high tumor distribution in mice with malignant pleural effusion, after intrapleural injection
title_sort	prolonged retention of liposomes in the pleural cavity of normal mice and high tumor distribution in mice with malignant pleural effusion, after intrapleural injection
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/7e9870be6cc8483cb8bdfd9b194402f3
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Prolonged retention of liposomes in the pleural cavity of normal mice and high tumor distribution in mice with malignant pleural effusion, after intrapleural injection

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