Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics

Sufa HI, Budiarti S, Rusmana I. 2018. Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics. Biodiversitas 19: 2359-2364. The aimed of this study was to identify, isolate and characterized th...

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Autores principales:	HAFIZAH ILMI SUFA, SRI BUDIARTI, IMAN RUSMANA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	biocontrol biodiversity lytic phage upec river contaminant urinary tract infection Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7e9e77f0fbea4991a941cbc864975ec9
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spelling	oai:doaj.org-article:7e9e77f0fbea4991a941cbc864975ec92021-11-16T14:04:40ZDiversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics1412-033X2085-472210.13057/biodiv/d190646https://doaj.org/article/7e9e77f0fbea4991a941cbc864975ec92018-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3242https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Sufa HI, Budiarti S, Rusmana I. 2018. Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics. Biodiversitas 19: 2359-2364. The aimed of this study was to identify, isolate and characterized the lytic phage diversity which had a potential in reducing uropathogenic Escherichia coli (UPEC), antibiotic resistant-pathogenic bacteria infecting urinary tract from Cisadane river based on the morphology and protein molecule weight. To the best of our knowledge, this study has not yet been reported in Indonesia. Two novel lytic phages of UPEC, FU1, and FU3 were isolated from Cisadane River, Indonesia. Both of lytic phages were more closely related to Podoviridae, which had icosahedral capsid in 47.2 nm and 40 nm diameter length, respectively and short-noncontractile tail (< 40 nm). The FU1 and FU3 had different protein molecular weight ranges between 10-76 kDa and 8-120 kDa, respectively. In addition, the bacteriolytic activity of FU3 (1.6 ´ 107 PFU/mL) was able to reduce UPEC (1.7 ´ 107 CFU/mL) to become lower than the limitation value of infection (< 105 CFU/mL) in only 25 h of incubation. A better effect of FU3 phage in decreasing UPEC population in vitro is expected to be one of the further alternative biocontrol agents to reduce the abundance of UPEC population which grows in urinary tract or contaminates river water.HAFIZAH ILMI SUFASRI BUDIARTIIMAN RUSMANAMBI & UNS Soloarticlebiocontrolbiodiversitylytic phageupecriver contaminanturinary tract infectionBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 6, Pp 2359-2364 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	biocontrol biodiversity lytic phage upec river contaminant urinary tract infection Biology (General) QH301-705.5
spellingShingle	biocontrol biodiversity lytic phage upec river contaminant urinary tract infection Biology (General) QH301-705.5 HAFIZAH ILMI SUFA SRI BUDIARTI IMAN RUSMANA Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics
description	Sufa HI, Budiarti S, Rusmana I. 2018. Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics. Biodiversitas 19: 2359-2364. The aimed of this study was to identify, isolate and characterized the lytic phage diversity which had a potential in reducing uropathogenic Escherichia coli (UPEC), antibiotic resistant-pathogenic bacteria infecting urinary tract from Cisadane river based on the morphology and protein molecule weight. To the best of our knowledge, this study has not yet been reported in Indonesia. Two novel lytic phages of UPEC, FU1, and FU3 were isolated from Cisadane River, Indonesia. Both of lytic phages were more closely related to Podoviridae, which had icosahedral capsid in 47.2 nm and 40 nm diameter length, respectively and short-noncontractile tail (< 40 nm). The FU1 and FU3 had different protein molecular weight ranges between 10-76 kDa and 8-120 kDa, respectively. In addition, the bacteriolytic activity of FU3 (1.6 ´ 107 PFU/mL) was able to reduce UPEC (1.7 ´ 107 CFU/mL) to become lower than the limitation value of infection (< 105 CFU/mL) in only 25 h of incubation. A better effect of FU3 phage in decreasing UPEC population in vitro is expected to be one of the further alternative biocontrol agents to reduce the abundance of UPEC population which grows in urinary tract or contaminates river water.
format	article
author	HAFIZAH ILMI SUFA SRI BUDIARTI IMAN RUSMANA
author_facet	HAFIZAH ILMI SUFA SRI BUDIARTI IMAN RUSMANA
author_sort	HAFIZAH ILMI SUFA
title	Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics
title_short	Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics
title_full	Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics
title_fullStr	Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics
title_full_unstemmed	Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics
title_sort	diversity of uropathogenic escherichia coli lytic phage from cisadane river, west java, indonesia based on morphology and protein molecular weight characteristics
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/7e9e77f0fbea4991a941cbc864975ec9
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_version_	1718426367790415872

Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics

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