Polarimetric imaging microscopy for advanced inspection of vegetal tissues

Abstract Optical microscopy techniques for plant inspection benefit from the fact that at least one of the multiple properties of light (intensity, phase, wavelength, polarization) may be modified by vegetal tissues. Paradoxically, polarimetric microscopy although being a mature technique in biophot...

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Autores principales:	Albert Van Eeckhout, Enrique Garcia-Caurel, Teresa Garnatje, Juan Carlos Escalera, Mercè Durfort, Josep Vidal, José J. Gil, Juan Campos, Angel Lizana
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ea675477a7a4bafbfb2daf0c0e0ec1b
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spelling	oai:doaj.org-article:7ea675477a7a4bafbfb2daf0c0e0ec1b2021-12-02T10:54:06ZPolarimetric imaging microscopy for advanced inspection of vegetal tissues10.1038/s41598-021-83421-82045-2322https://doaj.org/article/7ea675477a7a4bafbfb2daf0c0e0ec1b2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83421-8https://doaj.org/toc/2045-2322Abstract Optical microscopy techniques for plant inspection benefit from the fact that at least one of the multiple properties of light (intensity, phase, wavelength, polarization) may be modified by vegetal tissues. Paradoxically, polarimetric microscopy although being a mature technique in biophotonics, is not so commonly used in botany. Importantly, only specific polarimetric observables, as birefringence or dichroism, have some presence in botany studies, and other relevant metrics, as those based on depolarization, are underused. We present a versatile method, based on a representative selection of polarimetric observables, to obtain and to analyse images of plants which bring significant information about their structure and/or the spatial organization of their constituents (cells, organelles, among other structures). We provide a thorough analysis of polarimetric microscopy images of sections of plant leaves which are compared with those obtained by other commonly used microscopy techniques in plant biology. Our results show the interest of polarimetric microscopy for plant inspection, as it is non-destructive technique, highly competitive in economical and time consumption, and providing advantages compared to standard non-polarizing techniques.Albert Van EeckhoutEnrique Garcia-CaurelTeresa GarnatjeJuan Carlos EscaleraMercè DurfortJosep VidalJosé J. GilJuan CamposAngel LizanaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Albert Van Eeckhout Enrique Garcia-Caurel Teresa Garnatje Juan Carlos Escalera Mercè Durfort Josep Vidal José J. Gil Juan Campos Angel Lizana Polarimetric imaging microscopy for advanced inspection of vegetal tissues
description	Abstract Optical microscopy techniques for plant inspection benefit from the fact that at least one of the multiple properties of light (intensity, phase, wavelength, polarization) may be modified by vegetal tissues. Paradoxically, polarimetric microscopy although being a mature technique in biophotonics, is not so commonly used in botany. Importantly, only specific polarimetric observables, as birefringence or dichroism, have some presence in botany studies, and other relevant metrics, as those based on depolarization, are underused. We present a versatile method, based on a representative selection of polarimetric observables, to obtain and to analyse images of plants which bring significant information about their structure and/or the spatial organization of their constituents (cells, organelles, among other structures). We provide a thorough analysis of polarimetric microscopy images of sections of plant leaves which are compared with those obtained by other commonly used microscopy techniques in plant biology. Our results show the interest of polarimetric microscopy for plant inspection, as it is non-destructive technique, highly competitive in economical and time consumption, and providing advantages compared to standard non-polarizing techniques.
format	article
author	Albert Van Eeckhout Enrique Garcia-Caurel Teresa Garnatje Juan Carlos Escalera Mercè Durfort Josep Vidal José J. Gil Juan Campos Angel Lizana
author_facet	Albert Van Eeckhout Enrique Garcia-Caurel Teresa Garnatje Juan Carlos Escalera Mercè Durfort Josep Vidal José J. Gil Juan Campos Angel Lizana
author_sort	Albert Van Eeckhout
title	Polarimetric imaging microscopy for advanced inspection of vegetal tissues
title_short	Polarimetric imaging microscopy for advanced inspection of vegetal tissues
title_full	Polarimetric imaging microscopy for advanced inspection of vegetal tissues
title_fullStr	Polarimetric imaging microscopy for advanced inspection of vegetal tissues
title_full_unstemmed	Polarimetric imaging microscopy for advanced inspection of vegetal tissues
title_sort	polarimetric imaging microscopy for advanced inspection of vegetal tissues
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7ea675477a7a4bafbfb2daf0c0e0ec1b
work_keys_str_mv	AT albertvaneeckhout polarimetricimagingmicroscopyforadvancedinspectionofvegetaltissues AT enriquegarciacaurel polarimetricimagingmicroscopyforadvancedinspectionofvegetaltissues AT teresagarnatje polarimetricimagingmicroscopyforadvancedinspectionofvegetaltissues AT juancarlosescalera polarimetricimagingmicroscopyforadvancedinspectionofvegetaltissues AT mercedurfort polarimetricimagingmicroscopyforadvancedinspectionofvegetaltissues AT josepvidal polarimetricimagingmicroscopyforadvancedinspectionofvegetaltissues AT josejgil polarimetricimagingmicroscopyforadvancedinspectionofvegetaltissues AT juancampos polarimetricimagingmicroscopyforadvancedinspectionofvegetaltissues AT angellizana polarimetricimagingmicroscopyforadvancedinspectionofvegetaltissues
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Polarimetric imaging microscopy for advanced inspection of vegetal tissues

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