Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing

Liang Zhao,1,2,* Denglong Chen,2,3,* Qinghua Yao,2 Min Li2 1College of Life Sciences and Technology Xinxiang Medical University, Henan, 2College of Life Sciences, Fujian Normal University, Fuzhou, 3College of Environmental Science and Engineering, Fujian Normal University, Fuzhou, People’...

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Autores principales:	Zhao L, Chen D, Yao Q, Li M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	recombinant spider silk protein polyvinyl alcohol electrospinning characterization wound dressing Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7ead9503ebd0405b9b48db18cee86eb0
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spelling	oai:doaj.org-article:7ead9503ebd0405b9b48db18cee86eb02021-12-02T02:43:43ZStudies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing1178-2013https://doaj.org/article/7ead9503ebd0405b9b48db18cee86eb02017-11-01T00:00:00Zhttps://www.dovepress.com/studies-on-the-use-of-recombinant-spider-silk-proteinpolyvinyl-alcohol-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Liang Zhao,1,2,* Denglong Chen,2,3,* Qinghua Yao,2 Min Li2 1College of Life Sciences and Technology Xinxiang Medical University, Henan, 2College of Life Sciences, Fujian Normal University, Fuzhou, 3College of Environmental Science and Engineering, Fujian Normal University, Fuzhou, People’s Republic of China *These authors contributed equally to this work Abstract: A series of wound membranes of polyvinyl alcohol and recombinant spider silk protein (pNSR16) was prepared by electrospinning. The membrane was analyzed by scanning electron microscopy, atomic force microscopy, and Fourier transform infrared spectroscopy. The result showed that the three factors that affected average fiber diameter from high to low were, voltage, flow speed, and solidification distance; and the three factors that affected fiber uniformity from high to low were, flow speed, solidification distance, and voltage. The fibers adhered together after being dealt with alcohol. pNSR16 transformed from random coil into β-sheet after being immersed in alcohol. Additionally, the porosity of the electrospun membrane was 84.85%, which was higher than that of cast membrane prepared with the same composition. Experiments of applying electrospun membranes as wound dressing for Sprague Dawley rat wound healing showed that it could promote wound healing and basic fibroblast growth factor expression. Keywords: recombinant spider silk protein, polyvinyl alcohol, electrospinning, characterization, wound dressingZhao LChen DYao QLi MDove Medical Pressarticlerecombinant spider silk proteinpolyvinyl alcoholelectrospinningcharacterizationwound dressingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 8103-8114 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	recombinant spider silk protein polyvinyl alcohol electrospinning characterization wound dressing Medicine (General) R5-920
spellingShingle	recombinant spider silk protein polyvinyl alcohol electrospinning characterization wound dressing Medicine (General) R5-920 Zhao L Chen D Yao Q Li M Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
description	Liang Zhao,1,2,* Denglong Chen,2,3,* Qinghua Yao,2 Min Li2 1College of Life Sciences and Technology Xinxiang Medical University, Henan, 2College of Life Sciences, Fujian Normal University, Fuzhou, 3College of Environmental Science and Engineering, Fujian Normal University, Fuzhou, People’s Republic of China *These authors contributed equally to this work Abstract: A series of wound membranes of polyvinyl alcohol and recombinant spider silk protein (pNSR16) was prepared by electrospinning. The membrane was analyzed by scanning electron microscopy, atomic force microscopy, and Fourier transform infrared spectroscopy. The result showed that the three factors that affected average fiber diameter from high to low were, voltage, flow speed, and solidification distance; and the three factors that affected fiber uniformity from high to low were, flow speed, solidification distance, and voltage. The fibers adhered together after being dealt with alcohol. pNSR16 transformed from random coil into β-sheet after being immersed in alcohol. Additionally, the porosity of the electrospun membrane was 84.85%, which was higher than that of cast membrane prepared with the same composition. Experiments of applying electrospun membranes as wound dressing for Sprague Dawley rat wound healing showed that it could promote wound healing and basic fibroblast growth factor expression. Keywords: recombinant spider silk protein, polyvinyl alcohol, electrospinning, characterization, wound dressing
format	article
author	Zhao L Chen D Yao Q Li M
author_facet	Zhao L Chen D Yao Q Li M
author_sort	Zhao L
title	Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
title_short	Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
title_full	Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
title_fullStr	Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
title_full_unstemmed	Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
title_sort	studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/7ead9503ebd0405b9b48db18cee86eb0
work_keys_str_mv	AT zhaol studiesontheuseofrecombinantspidersilkproteinpolyvinylalcoholelectrospinningmembraneaswounddressing AT chend studiesontheuseofrecombinantspidersilkproteinpolyvinylalcoholelectrospinningmembraneaswounddressing AT yaoq studiesontheuseofrecombinantspidersilkproteinpolyvinylalcoholelectrospinningmembraneaswounddressing AT lim studiesontheuseofrecombinantspidersilkproteinpolyvinylalcoholelectrospinningmembraneaswounddressing
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Studies on the use of recombinant spider silk protein/polyvinyl alcohol electrospinning membrane as wound dressing

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