Effect of Withania somnifera hydroalcoholic extract and other dietary interventions in improving muscle strength in aging rats

Background: Aging leads to loss of skeletal muscle, diminished muscle strength, and decline in physical functions. Objective: This study evaluates Withania somnifera and some dietary interventions to combat muscle weakness in aging rats. Materials and methods: Rats (12–13 months old) corresponding t...

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Autores principales:	Vandana Panda, Amol Deshmukh, Asawari Hare, Sneha Singh, Lal Hingorani, S. Sudhamani
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Aging muscle Withania somnifera Protein Resistance exercise AMPK Bcl-2 and bax Miscellaneous systems and treatments RZ409.7-999
Acceso en línea:	https://doaj.org/article/7eb3e40a3b0c44bea8ab5148dee5766a
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spelling	oai:doaj.org-article:7eb3e40a3b0c44bea8ab5148dee5766a2021-12-02T04:59:18ZEffect of Withania somnifera hydroalcoholic extract and other dietary interventions in improving muscle strength in aging rats0975-947610.1016/j.jaim.2021.06.001https://doaj.org/article/7eb3e40a3b0c44bea8ab5148dee5766a2021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0975947621001091https://doaj.org/toc/0975-9476Background: Aging leads to loss of skeletal muscle, diminished muscle strength, and decline in physical functions. Objective: This study evaluates Withania somnifera and some dietary interventions to combat muscle weakness in aging rats. Materials and methods: Rats (12–13 months old) corresponding to a human age of 60–65 years were assigned to various groups and given orally a standardized W. somnifera extract (WSE, 500 mg/kg) or a protein cocktail comprising soybean (1.5 g/kg) and quinoa (1 g/kg) or a combination of WSE and the protein cocktail or whey protein (1 g/kg) as a reference standard or only resistance exercise for 60 days. Grip strength and blood glucose levels were monitored weekly. At the end of the treatment, total protein, inflammatory markers (CRP, IL-6 and TNF-α), AMPK, malondialdehyde, glutathione, antioxidant enzymes and apoptotic regulator genes (Bax and Bcl-2) were assayed. The biceps brachii muscle of all animals was subjected to histomorphological study. Results: All treatments successfully attenuated aging-elevated glucose, CRP, IL-6, TNF-α, AMPK, malondialdehyde, and Bax levels. A significant restoration of the aging-depleted total protein levels, glutathione, superoxide dismutase, catalase, and Bcl-2 was noted in the treatment groups. An increase in grip strength and greater biceps mass with all treatments indicated regaining of the frail aging muscle's strength and functionality. The WSE + protein treatment elicited the best results among all treatment groups to optimize muscle strength. Conclusion: All the interventions curbed muscle loss and strengthened the skeletal muscle by reducing inflammation, oxidative stress and apoptosis, and increasing ATP availability to the muscle.Vandana PandaAmol DeshmukhAsawari HareSneha SinghLal HingoraniS. SudhamaniElsevierarticleAging muscleWithania somniferaProteinResistance exerciseAMPKBcl-2 and baxMiscellaneous systems and treatmentsRZ409.7-999ENJournal of Ayurveda and Integrative Medicine, Vol 12, Iss 4, Pp 623-632 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aging muscle Withania somnifera Protein Resistance exercise AMPK Bcl-2 and bax Miscellaneous systems and treatments RZ409.7-999
spellingShingle	Aging muscle Withania somnifera Protein Resistance exercise AMPK Bcl-2 and bax Miscellaneous systems and treatments RZ409.7-999 Vandana Panda Amol Deshmukh Asawari Hare Sneha Singh Lal Hingorani S. Sudhamani Effect of Withania somnifera hydroalcoholic extract and other dietary interventions in improving muscle strength in aging rats
description	Background: Aging leads to loss of skeletal muscle, diminished muscle strength, and decline in physical functions. Objective: This study evaluates Withania somnifera and some dietary interventions to combat muscle weakness in aging rats. Materials and methods: Rats (12–13 months old) corresponding to a human age of 60–65 years were assigned to various groups and given orally a standardized W. somnifera extract (WSE, 500 mg/kg) or a protein cocktail comprising soybean (1.5 g/kg) and quinoa (1 g/kg) or a combination of WSE and the protein cocktail or whey protein (1 g/kg) as a reference standard or only resistance exercise for 60 days. Grip strength and blood glucose levels were monitored weekly. At the end of the treatment, total protein, inflammatory markers (CRP, IL-6 and TNF-α), AMPK, malondialdehyde, glutathione, antioxidant enzymes and apoptotic regulator genes (Bax and Bcl-2) were assayed. The biceps brachii muscle of all animals was subjected to histomorphological study. Results: All treatments successfully attenuated aging-elevated glucose, CRP, IL-6, TNF-α, AMPK, malondialdehyde, and Bax levels. A significant restoration of the aging-depleted total protein levels, glutathione, superoxide dismutase, catalase, and Bcl-2 was noted in the treatment groups. An increase in grip strength and greater biceps mass with all treatments indicated regaining of the frail aging muscle's strength and functionality. The WSE + protein treatment elicited the best results among all treatment groups to optimize muscle strength. Conclusion: All the interventions curbed muscle loss and strengthened the skeletal muscle by reducing inflammation, oxidative stress and apoptosis, and increasing ATP availability to the muscle.
format	article
author	Vandana Panda Amol Deshmukh Asawari Hare Sneha Singh Lal Hingorani S. Sudhamani
author_facet	Vandana Panda Amol Deshmukh Asawari Hare Sneha Singh Lal Hingorani S. Sudhamani
author_sort	Vandana Panda
title	Effect of Withania somnifera hydroalcoholic extract and other dietary interventions in improving muscle strength in aging rats
title_short	Effect of Withania somnifera hydroalcoholic extract and other dietary interventions in improving muscle strength in aging rats
title_full	Effect of Withania somnifera hydroalcoholic extract and other dietary interventions in improving muscle strength in aging rats
title_fullStr	Effect of Withania somnifera hydroalcoholic extract and other dietary interventions in improving muscle strength in aging rats
title_full_unstemmed	Effect of Withania somnifera hydroalcoholic extract and other dietary interventions in improving muscle strength in aging rats
title_sort	effect of withania somnifera hydroalcoholic extract and other dietary interventions in improving muscle strength in aging rats
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7eb3e40a3b0c44bea8ab5148dee5766a
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Effect of Withania somnifera hydroalcoholic extract and other dietary interventions in improving muscle strength in aging rats

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