Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays.

Biomaterial-driven modulation of cell adhesion and migration is a challenging aspect of tissue engineering. Here, we investigated the impact of surface-bound microgel arrays with variable geometry and adjustable cross-linking properties on cell adhesion and migration. We show that cell migration is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Janine Riegert, Alexander Töpel, Jana Schieren, Renee Coryn, Stella Dibenedetto, Dominik Braunmiller, Kamil Zajt, Carmen Schalla, Stephan Rütten, Martin Zenke, Andrij Pich, Antonio Sechi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7eb535e9432c4c7e8b44993fa0d5bac5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7eb535e9432c4c7e8b44993fa0d5bac5
record_format	dspace
spelling	oai:doaj.org-article:7eb535e9432c4c7e8b44993fa0d5bac52021-12-02T20:08:02ZGuiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays.1932-620310.1371/journal.pone.0257495https://doaj.org/article/7eb535e9432c4c7e8b44993fa0d5bac52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257495https://doaj.org/toc/1932-6203Biomaterial-driven modulation of cell adhesion and migration is a challenging aspect of tissue engineering. Here, we investigated the impact of surface-bound microgel arrays with variable geometry and adjustable cross-linking properties on cell adhesion and migration. We show that cell migration is inversely correlated with microgel array spacing, whereas directionality increases as array spacing increases. Focal adhesion dynamics is also modulated by microgel topography resulting in less dynamic focal adhesions on surface-bound microgels. Microgels also modulate the motility and adhesion of Sertoli cells used as a model for cell migration and adhesion. Both focal adhesion dynamics and speed are reduced on microgels. Interestingly, Gas2L1, a component of the cytoskeleton that mediates the interaction between microtubules and microfilaments, is dispensable for the regulation of cell adhesion and migration on microgels. Finally, increasing microgel cross-linking causes a clear reduction of focal adhesion turnover in Sertoli cells. These findings not only show that spacing and rigidity of surface-grafted microgels arrays can be effectively used to modulate cell adhesion and motility of diverse cellular systems, but they also form the basis for future developments in the fields of medicine and tissue engineering.Janine RiegertAlexander TöpelJana SchierenRenee CorynStella DibenedettoDominik BraunmillerKamil ZajtCarmen SchallaStephan RüttenMartin ZenkeAndrij PichAntonio SechiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257495 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Janine Riegert Alexander Töpel Jana Schieren Renee Coryn Stella Dibenedetto Dominik Braunmiller Kamil Zajt Carmen Schalla Stephan Rütten Martin Zenke Andrij Pich Antonio Sechi Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays.
description	Biomaterial-driven modulation of cell adhesion and migration is a challenging aspect of tissue engineering. Here, we investigated the impact of surface-bound microgel arrays with variable geometry and adjustable cross-linking properties on cell adhesion and migration. We show that cell migration is inversely correlated with microgel array spacing, whereas directionality increases as array spacing increases. Focal adhesion dynamics is also modulated by microgel topography resulting in less dynamic focal adhesions on surface-bound microgels. Microgels also modulate the motility and adhesion of Sertoli cells used as a model for cell migration and adhesion. Both focal adhesion dynamics and speed are reduced on microgels. Interestingly, Gas2L1, a component of the cytoskeleton that mediates the interaction between microtubules and microfilaments, is dispensable for the regulation of cell adhesion and migration on microgels. Finally, increasing microgel cross-linking causes a clear reduction of focal adhesion turnover in Sertoli cells. These findings not only show that spacing and rigidity of surface-grafted microgels arrays can be effectively used to modulate cell adhesion and motility of diverse cellular systems, but they also form the basis for future developments in the fields of medicine and tissue engineering.
format	article
author	Janine Riegert Alexander Töpel Jana Schieren Renee Coryn Stella Dibenedetto Dominik Braunmiller Kamil Zajt Carmen Schalla Stephan Rütten Martin Zenke Andrij Pich Antonio Sechi
author_facet	Janine Riegert Alexander Töpel Jana Schieren Renee Coryn Stella Dibenedetto Dominik Braunmiller Kamil Zajt Carmen Schalla Stephan Rütten Martin Zenke Andrij Pich Antonio Sechi
author_sort	Janine Riegert
title	Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays.
title_short	Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays.
title_full	Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays.
title_fullStr	Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays.
title_full_unstemmed	Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays.
title_sort	guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/7eb535e9432c4c7e8b44993fa0d5bac5
work_keys_str_mv	AT janineriegert guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays AT alexandertopel guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays AT janaschieren guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays AT reneecoryn guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays AT stelladibenedetto guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays AT dominikbraunmiller guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays AT kamilzajt guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays AT carmenschalla guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays AT stephanrutten guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays AT martinzenke guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays AT andrijpich guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays AT antoniosechi guidingcelladhesionandmotilitybymodulatingcrosslinkingandtopographicpropertiesofmicrogelarrays
_version_	1718375252149403648

Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays.

Ejemplares similares