A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

CRISPR interference (CRISPRi) is a method for targeted silencing of transcription that requires the coexpression of protein dCas9 and a customized guide RNA. Here, Cui et al. show that certain guide RNAs induce toxicity in E. coli, and provide design rules to minimize off-target effects.

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Autores principales:	Lun Cui, Antoine Vigouroux, François Rousset, Hugo Varet, Varun Khanna, David Bikard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7ebd4461f9244907aa4954aebfa7e9b5
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spelling	oai:doaj.org-article:7ebd4461f9244907aa4954aebfa7e9b52021-12-02T14:39:46ZA CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas910.1038/s41467-018-04209-52041-1723https://doaj.org/article/7ebd4461f9244907aa4954aebfa7e9b52018-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04209-5https://doaj.org/toc/2041-1723CRISPR interference (CRISPRi) is a method for targeted silencing of transcription that requires the coexpression of protein dCas9 and a customized guide RNA. Here, Cui et al. show that certain guide RNAs induce toxicity in E. coli, and provide design rules to minimize off-target effects.Lun CuiAntoine VigourouxFrançois RoussetHugo VaretVarun KhannaDavid BikardNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Lun Cui Antoine Vigouroux François Rousset Hugo Varet Varun Khanna David Bikard A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
description	CRISPR interference (CRISPRi) is a method for targeted silencing of transcription that requires the coexpression of protein dCas9 and a customized guide RNA. Here, Cui et al. show that certain guide RNAs induce toxicity in E. coli, and provide design rules to minimize off-target effects.
format	article
author	Lun Cui Antoine Vigouroux François Rousset Hugo Varet Varun Khanna David Bikard
author_facet	Lun Cui Antoine Vigouroux François Rousset Hugo Varet Varun Khanna David Bikard
author_sort	Lun Cui
title	A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
title_short	A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
title_full	A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
title_fullStr	A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
title_full_unstemmed	A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
title_sort	crispri screen in e. coli reveals sequence-specific toxicity of dcas9
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7ebd4461f9244907aa4954aebfa7e9b5
work_keys_str_mv	AT luncui acrispriscreeninecolirevealssequencespecifictoxicityofdcas9 AT antoinevigouroux acrispriscreeninecolirevealssequencespecifictoxicityofdcas9 AT francoisrousset acrispriscreeninecolirevealssequencespecifictoxicityofdcas9 AT hugovaret acrispriscreeninecolirevealssequencespecifictoxicityofdcas9 AT varunkhanna acrispriscreeninecolirevealssequencespecifictoxicityofdcas9 AT davidbikard acrispriscreeninecolirevealssequencespecifictoxicityofdcas9 AT luncui crispriscreeninecolirevealssequencespecifictoxicityofdcas9 AT antoinevigouroux crispriscreeninecolirevealssequencespecifictoxicityofdcas9 AT francoisrousset crispriscreeninecolirevealssequencespecifictoxicityofdcas9 AT hugovaret crispriscreeninecolirevealssequencespecifictoxicityofdcas9 AT varunkhanna crispriscreeninecolirevealssequencespecifictoxicityofdcas9 AT davidbikard crispriscreeninecolirevealssequencespecifictoxicityofdcas9
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A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

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