Cell culture techniques in gastrointestinal research: Methods, possibilities and challenges

Cell culture is one of the most valuable tools which is being applied in both fundamental and applied gastrointestinal research. The cells are isolated from their natural location (in vivo) and further propagated in vitro or artificial environment and studied. Over the years, several methods have be...

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Autores principales:	Gaur Preksha, Rajendran Yesheswini, Chittur V Srikanth
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	2d culture cell lines co-culture contamination organ on chip organotypic cultures Pathology RB1-214 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7ec37dcd03334bccafb9abe1958ecc4c
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spelling	oai:doaj.org-article:7ec37dcd03334bccafb9abe1958ecc4c2021-12-02T19:27:26ZCell culture techniques in gastrointestinal research: Methods, possibilities and challenges0377-492910.4103/IJPM.IJPM_933_20https://doaj.org/article/7ec37dcd03334bccafb9abe1958ecc4c2021-01-01T00:00:00Zhttp://www.ijpmonline.org/article.asp?issn=0377-4929;year=2021;volume=64;issue=5;spage=52;epage=57;aulast=Prekshahttps://doaj.org/toc/0377-4929Cell culture is one of the most valuable tools which is being applied in both fundamental and applied gastrointestinal research. The cells are isolated from their natural location (in vivo) and further propagated in vitro or artificial environment and studied. Over the years, several methods have been devised to isolate animal cells derived from the gut and culture them in vitro to study the functions and biology in the context of complex gastrointestinal diseases. This mini-review briefly describes the types and methods of cell culture covering the simplest monoculture models to more recent 3D organoid models, highlighting its importance in personalized precession medicine and other aspects of translational research. It also throws light upon the major challenges and outlines the future directions for using cell culture as a model system.Gaur PrekshaRajendran YesheswiniChittur V SrikanthWolters Kluwer Medknow Publicationsarticle2d culturecell linesco-culturecontaminationorgan on chiporganotypic culturesPathologyRB1-214MicrobiologyQR1-502ENIndian Journal of Pathology and Microbiology, Vol 64, Iss 5, Pp 52-57 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	2d culture cell lines co-culture contamination organ on chip organotypic cultures Pathology RB1-214 Microbiology QR1-502
spellingShingle	2d culture cell lines co-culture contamination organ on chip organotypic cultures Pathology RB1-214 Microbiology QR1-502 Gaur Preksha Rajendran Yesheswini Chittur V Srikanth Cell culture techniques in gastrointestinal research: Methods, possibilities and challenges
description	Cell culture is one of the most valuable tools which is being applied in both fundamental and applied gastrointestinal research. The cells are isolated from their natural location (in vivo) and further propagated in vitro or artificial environment and studied. Over the years, several methods have been devised to isolate animal cells derived from the gut and culture them in vitro to study the functions and biology in the context of complex gastrointestinal diseases. This mini-review briefly describes the types and methods of cell culture covering the simplest monoculture models to more recent 3D organoid models, highlighting its importance in personalized precession medicine and other aspects of translational research. It also throws light upon the major challenges and outlines the future directions for using cell culture as a model system.
format	article
author	Gaur Preksha Rajendran Yesheswini Chittur V Srikanth
author_facet	Gaur Preksha Rajendran Yesheswini Chittur V Srikanth
author_sort	Gaur Preksha
title	Cell culture techniques in gastrointestinal research: Methods, possibilities and challenges
title_short	Cell culture techniques in gastrointestinal research: Methods, possibilities and challenges
title_full	Cell culture techniques in gastrointestinal research: Methods, possibilities and challenges
title_fullStr	Cell culture techniques in gastrointestinal research: Methods, possibilities and challenges
title_full_unstemmed	Cell culture techniques in gastrointestinal research: Methods, possibilities and challenges
title_sort	cell culture techniques in gastrointestinal research: methods, possibilities and challenges
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/7ec37dcd03334bccafb9abe1958ecc4c
work_keys_str_mv	AT gaurpreksha cellculturetechniquesingastrointestinalresearchmethodspossibilitiesandchallenges AT rajendranyesheswini cellculturetechniquesingastrointestinalresearchmethodspossibilitiesandchallenges AT chitturvsrikanth cellculturetechniquesingastrointestinalresearchmethodspossibilitiesandchallenges
_version_	1718376508195602432

Cell culture techniques in gastrointestinal research: Methods, possibilities and challenges

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