Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design

Abstract Porcine epidemic diarrhea virus (PEDV), as the main causative pathogen of viral diarrhea in pigs, has been reported to result in high morbidity and mortality in neonatal piglets and cause significant economic losses to the swine industry. Rapid diagnosis methods are essential for preventing...

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Autores principales:	Siyi Zou, Lei Wu, Gan Li, Juan Wang, Dongni Cao, Tao Xu, Aiqing Jia, Yong Tang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Porcine epidemic diarrhea virus Latex beads Lateral flow immunoassay Sample pretreatment Filter pad Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/7ec3fc6d7a3b4f99af454f98432f1d8b
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spelling	oai:doaj.org-article:7ec3fc6d7a3b4f99af454f98432f1d8b2021-11-14T12:06:19ZDevelopment of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design10.1186/s44149-021-00029-12731-0442https://doaj.org/article/7ec3fc6d7a3b4f99af454f98432f1d8b2021-11-01T00:00:00Zhttps://doi.org/10.1186/s44149-021-00029-1https://doaj.org/toc/2731-0442Abstract Porcine epidemic diarrhea virus (PEDV), as the main causative pathogen of viral diarrhea in pigs, has been reported to result in high morbidity and mortality in neonatal piglets and cause significant economic losses to the swine industry. Rapid diagnosis methods are essential for preventing outbreaks and transmission of this disease. In this study, a paper-based lateral flow immunoassay for the rapid diagnosis of PEDV in swine fecal samples was developed using stable color-rich latex beads as the label. Under optimal conditions, the newly developed latex bead-based lateral flow immunoassay (LBs-LFIA) attained a limit of detection (LOD) as low as 103.60 TCID50/mL and no cross-reactivity with other related swine viruses. To solve swine feces impurity interference, by adding a filtration unit design of LFIA without an additional pretreatment procedure, the LBs-LFIA gave good agreement (92.59%) with RT-PCR results in the analysis of clinical swine fecal samples (n = 108), which was more accurate than previously reported colloidal gold LFIA (74.07%) and fluorescent LFIA (86.67%). Moreover, LBs-LFIA showed sufficient accuracy (coefficient of variance [CV] < 15%) and stable (room temperature storage life > 56 days) performance for PEDV detection, which is promising for on-site analysis and user-driven testing in pig production system.Siyi ZouLei WuGan LiJuan WangDongni CaoTao XuAiqing JiaYong TangBMCarticlePorcine epidemic diarrhea virusLatex beadsLateral flow immunoassaySample pretreatmentFilter padVeterinary medicineSF600-1100Public aspects of medicineRA1-1270ENAnimal Diseases, Vol 1, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Porcine epidemic diarrhea virus Latex beads Lateral flow immunoassay Sample pretreatment Filter pad Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
spellingShingle	Porcine epidemic diarrhea virus Latex beads Lateral flow immunoassay Sample pretreatment Filter pad Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270 Siyi Zou Lei Wu Gan Li Juan Wang Dongni Cao Tao Xu Aiqing Jia Yong Tang Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design
description	Abstract Porcine epidemic diarrhea virus (PEDV), as the main causative pathogen of viral diarrhea in pigs, has been reported to result in high morbidity and mortality in neonatal piglets and cause significant economic losses to the swine industry. Rapid diagnosis methods are essential for preventing outbreaks and transmission of this disease. In this study, a paper-based lateral flow immunoassay for the rapid diagnosis of PEDV in swine fecal samples was developed using stable color-rich latex beads as the label. Under optimal conditions, the newly developed latex bead-based lateral flow immunoassay (LBs-LFIA) attained a limit of detection (LOD) as low as 103.60 TCID50/mL and no cross-reactivity with other related swine viruses. To solve swine feces impurity interference, by adding a filtration unit design of LFIA without an additional pretreatment procedure, the LBs-LFIA gave good agreement (92.59%) with RT-PCR results in the analysis of clinical swine fecal samples (n = 108), which was more accurate than previously reported colloidal gold LFIA (74.07%) and fluorescent LFIA (86.67%). Moreover, LBs-LFIA showed sufficient accuracy (coefficient of variance [CV] < 15%) and stable (room temperature storage life > 56 days) performance for PEDV detection, which is promising for on-site analysis and user-driven testing in pig production system.
format	article
author	Siyi Zou Lei Wu Gan Li Juan Wang Dongni Cao Tao Xu Aiqing Jia Yong Tang
author_facet	Siyi Zou Lei Wu Gan Li Juan Wang Dongni Cao Tao Xu Aiqing Jia Yong Tang
author_sort	Siyi Zou
title	Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design
title_short	Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design
title_full	Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design
title_fullStr	Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design
title_full_unstemmed	Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design
title_sort	development of an accurate lateral flow immunoassay for pedv detection in swine fecal samples with a filter pad design
publisher	BMC
publishDate	2021
url	https://doaj.org/article/7ec3fc6d7a3b4f99af454f98432f1d8b
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Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design

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