Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al. show that CRISPR/Cas9 ribonucleoproteins can be used to generate genome edited wheat plants in as little as nine weeks.

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Autores principales: Zhen Liang, Kunling Chen, Tingdong Li, Yi Zhang, Yanpeng Wang, Qian Zhao, Jinxing Liu, Huawei Zhang, Cuimin Liu, Yidong Ran, Caixia Gao
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/7eca31ccd8a84cdba06a1486c2d46592
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spelling oai:doaj.org-article:7eca31ccd8a84cdba06a1486c2d465922021-12-02T17:06:18ZEfficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes10.1038/ncomms142612041-1723https://doaj.org/article/7eca31ccd8a84cdba06a1486c2d465922017-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms14261https://doaj.org/toc/2041-1723Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al. show that CRISPR/Cas9 ribonucleoproteins can be used to generate genome edited wheat plants in as little as nine weeks.Zhen LiangKunling ChenTingdong LiYi ZhangYanpeng WangQian ZhaoJinxing LiuHuawei ZhangCuimin LiuYidong RanCaixia GaoNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-5 (2017)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Zhen Liang
Kunling Chen
Tingdong Li
Yi Zhang
Yanpeng Wang
Qian Zhao
Jinxing Liu
Huawei Zhang
Cuimin Liu
Yidong Ran
Caixia Gao
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
description Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al. show that CRISPR/Cas9 ribonucleoproteins can be used to generate genome edited wheat plants in as little as nine weeks.
format article
author Zhen Liang
Kunling Chen
Tingdong Li
Yi Zhang
Yanpeng Wang
Qian Zhao
Jinxing Liu
Huawei Zhang
Cuimin Liu
Yidong Ran
Caixia Gao
author_facet Zhen Liang
Kunling Chen
Tingdong Li
Yi Zhang
Yanpeng Wang
Qian Zhao
Jinxing Liu
Huawei Zhang
Cuimin Liu
Yidong Ran
Caixia Gao
author_sort Zhen Liang
title Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
title_short Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
title_full Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
title_fullStr Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
title_full_unstemmed Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
title_sort efficient dna-free genome editing of bread wheat using crispr/cas9 ribonucleoprotein complexes
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/7eca31ccd8a84cdba06a1486c2d46592
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