Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al. show that CRISPR/Cas9 ribonucleoproteins can be used to generate genome edited wheat plants in as little as nine weeks.
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Nature Portfolio
2017
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oai:doaj.org-article:7eca31ccd8a84cdba06a1486c2d465922021-12-02T17:06:18ZEfficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes10.1038/ncomms142612041-1723https://doaj.org/article/7eca31ccd8a84cdba06a1486c2d465922017-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms14261https://doaj.org/toc/2041-1723Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al. show that CRISPR/Cas9 ribonucleoproteins can be used to generate genome edited wheat plants in as little as nine weeks.Zhen LiangKunling ChenTingdong LiYi ZhangYanpeng WangQian ZhaoJinxing LiuHuawei ZhangCuimin LiuYidong RanCaixia GaoNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-5 (2017) |
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Science Q Zhen Liang Kunling Chen Tingdong Li Yi Zhang Yanpeng Wang Qian Zhao Jinxing Liu Huawei Zhang Cuimin Liu Yidong Ran Caixia Gao Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes |
description |
Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al. show that CRISPR/Cas9 ribonucleoproteins can be used to generate genome edited wheat plants in as little as nine weeks. |
format |
article |
author |
Zhen Liang Kunling Chen Tingdong Li Yi Zhang Yanpeng Wang Qian Zhao Jinxing Liu Huawei Zhang Cuimin Liu Yidong Ran Caixia Gao |
author_facet |
Zhen Liang Kunling Chen Tingdong Li Yi Zhang Yanpeng Wang Qian Zhao Jinxing Liu Huawei Zhang Cuimin Liu Yidong Ran Caixia Gao |
author_sort |
Zhen Liang |
title |
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes |
title_short |
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes |
title_full |
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes |
title_fullStr |
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes |
title_full_unstemmed |
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes |
title_sort |
efficient dna-free genome editing of bread wheat using crispr/cas9 ribonucleoprotein complexes |
publisher |
Nature Portfolio |
publishDate |
2017 |
url |
https://doaj.org/article/7eca31ccd8a84cdba06a1486c2d46592 |
work_keys_str_mv |
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