Swiprosin-1 deficiency in macrophages alleviated atherogenesis

Abstract Macrophages play a vital role in the development of atherosclerosis. Previously, we have found that swiprosin-1 was abundantly expressed in macrophages. Here, we investigated the role of swiprosin-1 expressed in macrophages in atherogenesis. Bone marrow transplantation was performed from sw...

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Autores principales:	Ling-Chang Tong, Zhi-Bin Wang, Jia-Qi Zhang, Yue Wang, Wei-Ye Liu, Hao Yin, Jia-Cheng Li, Ding-Feng Su, Yong-Bing Cao, Li-Chao Zhang, Ling Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Publishing Group 2021
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/7eca3c2e0311419ba0bfd78fb4d626e1
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spelling	oai:doaj.org-article:7eca3c2e0311419ba0bfd78fb4d626e12021-11-14T12:12:38ZSwiprosin-1 deficiency in macrophages alleviated atherogenesis10.1038/s41420-021-00739-y2058-7716https://doaj.org/article/7eca3c2e0311419ba0bfd78fb4d626e12021-11-01T00:00:00Zhttps://doi.org/10.1038/s41420-021-00739-yhttps://doaj.org/toc/2058-7716Abstract Macrophages play a vital role in the development of atherosclerosis. Previously, we have found that swiprosin-1 was abundantly expressed in macrophages. Here, we investigated the role of swiprosin-1 expressed in macrophages in atherogenesis. Bone marrow transplantation was performed from swiprosin-1-knockout (Swp −/− ) mice and age-matched ApoE −/− mice. Atherosclerotic lesion, serum lipid, and interleukin-β (IL-β) levels were detected. In vitro, the peritoneal macrophages isolated from Swp −/− and wild-type mice were stimulated with oxidized low-density lipoprotein (ox-LDL) and the macrophage of foam degree, cellular lipid content, apoptosis, inflammatory factor, migration, and autophagy were determined. Our results showed that swiprosin-1 was mainly expressed in macrophages of atherosclerotic plaques in aorta from ApoE −/− mice fed with high-cholesterol diet (HCD). The expression of swiprosin-1 in the foaming of RAW264.7 macrophages gradually increased with the increase of the concentration and time stimulated with ox-LDL. Atherosclerotic plaques, accumulation of macrophages, collagen content, serum total cholesterol, LDL, and IL-β levels were decreased in Swp − /− → ApoE −/− mice compared with Swp +/+ → ApoE −/− mice fed with HCD for 16 weeks. The macrophage foam cell formation and cellular cholesterol accumulation were reduced, while the lipid uptake and efflux increased in macrophages isolated from Swp −/− compared to wild-type mice treated with ox-LDL. Swiprosin-1 deficiency in macrophages could inhibit apoptosis, inflammation, migration, and promote autophagy. Taken together, our results demonstrated that swiprosin-1 deficiency in macrophages could alleviate the development and progression of AS. The role of swiprosin-1 may provide a promising new target for ameliorating AS.Ling-Chang TongZhi-Bin WangJia-Qi ZhangYue WangWei-Ye LiuHao YinJia-Cheng LiDing-Feng SuYong-Bing CaoLi-Chao ZhangLing LiNature Publishing GrouparticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282CytologyQH573-671ENCell Death Discovery, Vol 7, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671 Ling-Chang Tong Zhi-Bin Wang Jia-Qi Zhang Yue Wang Wei-Ye Liu Hao Yin Jia-Cheng Li Ding-Feng Su Yong-Bing Cao Li-Chao Zhang Ling Li Swiprosin-1 deficiency in macrophages alleviated atherogenesis
description	Abstract Macrophages play a vital role in the development of atherosclerosis. Previously, we have found that swiprosin-1 was abundantly expressed in macrophages. Here, we investigated the role of swiprosin-1 expressed in macrophages in atherogenesis. Bone marrow transplantation was performed from swiprosin-1-knockout (Swp −/− ) mice and age-matched ApoE −/− mice. Atherosclerotic lesion, serum lipid, and interleukin-β (IL-β) levels were detected. In vitro, the peritoneal macrophages isolated from Swp −/− and wild-type mice were stimulated with oxidized low-density lipoprotein (ox-LDL) and the macrophage of foam degree, cellular lipid content, apoptosis, inflammatory factor, migration, and autophagy were determined. Our results showed that swiprosin-1 was mainly expressed in macrophages of atherosclerotic plaques in aorta from ApoE −/− mice fed with high-cholesterol diet (HCD). The expression of swiprosin-1 in the foaming of RAW264.7 macrophages gradually increased with the increase of the concentration and time stimulated with ox-LDL. Atherosclerotic plaques, accumulation of macrophages, collagen content, serum total cholesterol, LDL, and IL-β levels were decreased in Swp − /− → ApoE −/− mice compared with Swp +/+ → ApoE −/− mice fed with HCD for 16 weeks. The macrophage foam cell formation and cellular cholesterol accumulation were reduced, while the lipid uptake and efflux increased in macrophages isolated from Swp −/− compared to wild-type mice treated with ox-LDL. Swiprosin-1 deficiency in macrophages could inhibit apoptosis, inflammation, migration, and promote autophagy. Taken together, our results demonstrated that swiprosin-1 deficiency in macrophages could alleviate the development and progression of AS. The role of swiprosin-1 may provide a promising new target for ameliorating AS.
format	article
author	Ling-Chang Tong Zhi-Bin Wang Jia-Qi Zhang Yue Wang Wei-Ye Liu Hao Yin Jia-Cheng Li Ding-Feng Su Yong-Bing Cao Li-Chao Zhang Ling Li
author_facet	Ling-Chang Tong Zhi-Bin Wang Jia-Qi Zhang Yue Wang Wei-Ye Liu Hao Yin Jia-Cheng Li Ding-Feng Su Yong-Bing Cao Li-Chao Zhang Ling Li
author_sort	Ling-Chang Tong
title	Swiprosin-1 deficiency in macrophages alleviated atherogenesis
title_short	Swiprosin-1 deficiency in macrophages alleviated atherogenesis
title_full	Swiprosin-1 deficiency in macrophages alleviated atherogenesis
title_fullStr	Swiprosin-1 deficiency in macrophages alleviated atherogenesis
title_full_unstemmed	Swiprosin-1 deficiency in macrophages alleviated atherogenesis
title_sort	swiprosin-1 deficiency in macrophages alleviated atherogenesis
publisher	Nature Publishing Group
publishDate	2021
url	https://doaj.org/article/7eca3c2e0311419ba0bfd78fb4d626e1
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Swiprosin-1 deficiency in macrophages alleviated atherogenesis

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