Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX

Abstract Excessive secretion of glucagon, a functional insulin antagonist, significantly contributes to hyperglycemia. Glucagon exerts its physiological functions through activation of the glucagon receptor (GCGR). Inhibition of GCGR activity represents a potential therapeutic approach for reducing...

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Autores principales:	Guodong Wang, Jun Liu, Ke Chen, Yiling Xu, Bo Liu, Jie Liao, Lei Zhu, Xiaoxiao Hu, Jianglin Li, Ying Pu, Wen Zhong, Ting Fu, Huixia Liu, Weihong Tan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ecb0a7671774d3482376e2930725473
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spelling	oai:doaj.org-article:7ecb0a7671774d3482376e29307254732021-12-02T11:41:10ZSelection and characterization of DNA aptamer against glucagon receptor by cell-SELEX10.1038/s41598-017-05840-w2045-2322https://doaj.org/article/7ecb0a7671774d3482376e29307254732017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05840-whttps://doaj.org/toc/2045-2322Abstract Excessive secretion of glucagon, a functional insulin antagonist, significantly contributes to hyperglycemia. Glucagon exerts its physiological functions through activation of the glucagon receptor (GCGR). Inhibition of GCGR activity represents a potential therapeutic approach for reducing excess glucose production in diabetes mellitus. Aptamers are short DNA or RNA oligonucleotides evolved from systematic evolution of ligands by exponential enrichment (SELEX). Here, we have successfully selected a DNA aptamer against GCGR by cell-SELEX, which can specifically bind membrane protein of CHO-GCGR cells with a K d of 52.7 ± 5.1 nM. Aptamer-mediated pull-down and gcgr knockdown assay verified that GCGR was the target of aptamer GR-3. Binding analysis revealed that GR-3 could recognize other cells with different affinity according to the level of GCGR protein expressed in these cells. Hepatic tissue imaging suggested that GR-3 could bind the cell membrane of hepatic tissues. With the advantages of small size, high binding affinity, good stability, lack of immunogenicity, and easy synthesis, aptamer GR-3 against GCGR can be a promising tool with the potential to attenuate hyperglycemia in diabetes mellitus.Guodong WangJun LiuKe ChenYiling XuBo LiuJie LiaoLei ZhuXiaoxiao HuJianglin LiYing PuWen ZhongTing FuHuixia LiuWeihong TanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guodong Wang Jun Liu Ke Chen Yiling Xu Bo Liu Jie Liao Lei Zhu Xiaoxiao Hu Jianglin Li Ying Pu Wen Zhong Ting Fu Huixia Liu Weihong Tan Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX
description	Abstract Excessive secretion of glucagon, a functional insulin antagonist, significantly contributes to hyperglycemia. Glucagon exerts its physiological functions through activation of the glucagon receptor (GCGR). Inhibition of GCGR activity represents a potential therapeutic approach for reducing excess glucose production in diabetes mellitus. Aptamers are short DNA or RNA oligonucleotides evolved from systematic evolution of ligands by exponential enrichment (SELEX). Here, we have successfully selected a DNA aptamer against GCGR by cell-SELEX, which can specifically bind membrane protein of CHO-GCGR cells with a K d of 52.7 ± 5.1 nM. Aptamer-mediated pull-down and gcgr knockdown assay verified that GCGR was the target of aptamer GR-3. Binding analysis revealed that GR-3 could recognize other cells with different affinity according to the level of GCGR protein expressed in these cells. Hepatic tissue imaging suggested that GR-3 could bind the cell membrane of hepatic tissues. With the advantages of small size, high binding affinity, good stability, lack of immunogenicity, and easy synthesis, aptamer GR-3 against GCGR can be a promising tool with the potential to attenuate hyperglycemia in diabetes mellitus.
format	article
author	Guodong Wang Jun Liu Ke Chen Yiling Xu Bo Liu Jie Liao Lei Zhu Xiaoxiao Hu Jianglin Li Ying Pu Wen Zhong Ting Fu Huixia Liu Weihong Tan
author_facet	Guodong Wang Jun Liu Ke Chen Yiling Xu Bo Liu Jie Liao Lei Zhu Xiaoxiao Hu Jianglin Li Ying Pu Wen Zhong Ting Fu Huixia Liu Weihong Tan
author_sort	Guodong Wang
title	Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX
title_short	Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX
title_full	Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX
title_fullStr	Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX
title_full_unstemmed	Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX
title_sort	selection and characterization of dna aptamer against glucagon receptor by cell-selex
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7ecb0a7671774d3482376e2930725473
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Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX

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