Intratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice

Abstract Botulinum neurotoxin (BoNT), produced by Clostridium botulinum, is generally known to be the most poisonous of all biological toxins. In this study, we evaluate the protection conferred by intratracheal (i.t.) inoculation immunization with recombinant Hc subunit (AHc) vaccines against aeros...

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Autores principales:	Changjiao Gan, Wenbo Luo, Yunzhou Yu, Zhouguang Jiao, Sha Li, Duo Su, Junxia Feng, Xiaodong Zhao, Yefeng Qiu, Lingfei Hu, Dongsheng Zhou, Xiaolu Xiong, Jinglin Wang, Huiying Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/7ecfa95b4a8e41dfbba20ce2f3724ce7
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spelling	oai:doaj.org-article:7ecfa95b4a8e41dfbba20ce2f3724ce72021-12-02T18:02:54ZIntratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice10.1038/s41541-021-00349-w2059-0105https://doaj.org/article/7ecfa95b4a8e41dfbba20ce2f3724ce72021-06-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00349-whttps://doaj.org/toc/2059-0105Abstract Botulinum neurotoxin (BoNT), produced by Clostridium botulinum, is generally known to be the most poisonous of all biological toxins. In this study, we evaluate the protection conferred by intratracheal (i.t.) inoculation immunization with recombinant Hc subunit (AHc) vaccines against aerosolized BoNT/A intoxication. Three AHc vaccine formulations, i.e., conventional liquid, dry powder produced by spray freeze drying, and AHc dry powder reconstituted in water are prepared, and mice are immunized via i.t. inoculation or subcutaneous (s.c.) injection. Compared with s.c.-AHc-immunized mice, i.t.-AHc-immunized mice exhibit a slightly stronger protection against a challenge with 30,000× LD50 aerosolized BoNT/A. Of note, only i.t.-AHc induces a significantly higher level of toxin-neutralizing mucosal secretory IgA (SIgA) production in the bronchoalveolar lavage of mice. In conclusion, our study demonstrates that the immune protection conferred by the three formulations of AHc is comparable, while i.t. immunization of AHc is superior to s.c. immunization against aerosolized BoNT/A intoxication.Changjiao GanWenbo LuoYunzhou YuZhouguang JiaoSha LiDuo SuJunxia FengXiaodong ZhaoYefeng QiuLingfei HuDongsheng ZhouXiaolu XiongJinglin WangHuiying YangNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Changjiao Gan Wenbo Luo Yunzhou Yu Zhouguang Jiao Sha Li Duo Su Junxia Feng Xiaodong Zhao Yefeng Qiu Lingfei Hu Dongsheng Zhou Xiaolu Xiong Jinglin Wang Huiying Yang Intratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice
description	Abstract Botulinum neurotoxin (BoNT), produced by Clostridium botulinum, is generally known to be the most poisonous of all biological toxins. In this study, we evaluate the protection conferred by intratracheal (i.t.) inoculation immunization with recombinant Hc subunit (AHc) vaccines against aerosolized BoNT/A intoxication. Three AHc vaccine formulations, i.e., conventional liquid, dry powder produced by spray freeze drying, and AHc dry powder reconstituted in water are prepared, and mice are immunized via i.t. inoculation or subcutaneous (s.c.) injection. Compared with s.c.-AHc-immunized mice, i.t.-AHc-immunized mice exhibit a slightly stronger protection against a challenge with 30,000× LD50 aerosolized BoNT/A. Of note, only i.t.-AHc induces a significantly higher level of toxin-neutralizing mucosal secretory IgA (SIgA) production in the bronchoalveolar lavage of mice. In conclusion, our study demonstrates that the immune protection conferred by the three formulations of AHc is comparable, while i.t. immunization of AHc is superior to s.c. immunization against aerosolized BoNT/A intoxication.
format	article
author	Changjiao Gan Wenbo Luo Yunzhou Yu Zhouguang Jiao Sha Li Duo Su Junxia Feng Xiaodong Zhao Yefeng Qiu Lingfei Hu Dongsheng Zhou Xiaolu Xiong Jinglin Wang Huiying Yang
author_facet	Changjiao Gan Wenbo Luo Yunzhou Yu Zhouguang Jiao Sha Li Duo Su Junxia Feng Xiaodong Zhao Yefeng Qiu Lingfei Hu Dongsheng Zhou Xiaolu Xiong Jinglin Wang Huiying Yang
author_sort	Changjiao Gan
title	Intratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice
title_short	Intratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice
title_full	Intratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice
title_fullStr	Intratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice
title_full_unstemmed	Intratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice
title_sort	intratracheal inoculation of ahc vaccine induces protection against aerosolized botulinum neurotoxin a challenge in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7ecfa95b4a8e41dfbba20ce2f3724ce7
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Intratracheal inoculation of AHc vaccine induces protection against aerosolized botulinum neurotoxin A challenge in mice

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