Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons

Abstract The spinal dorsal horn harbors a sophisticated and heterogeneous network of excitatory and inhibitory neurons that process peripheral signals encoding different sensory modalities. Although it has long been recognized that this network is crucial both for the separation and the integration...

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Autores principales:	Rebecca Rani Das Gupta, Louis Scheurer, Pawel Pelczar, Hendrik Wildner, Hanns Ulrich Zeilhofer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ed2f83328d34b1d85c69a4326b33081
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spelling	oai:doaj.org-article:7ed2f83328d34b1d85c69a4326b330812021-12-02T13:30:11ZNeuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons10.1038/s41598-021-84667-y2045-2322https://doaj.org/article/7ed2f83328d34b1d85c69a4326b330812021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84667-yhttps://doaj.org/toc/2045-2322Abstract The spinal dorsal horn harbors a sophisticated and heterogeneous network of excitatory and inhibitory neurons that process peripheral signals encoding different sensory modalities. Although it has long been recognized that this network is crucial both for the separation and the integration of sensory signals of different modalities, a systematic unbiased approach to the use of specific neuromodulatory systems is still missing. Here, we have used the translating ribosome affinity purification (TRAP) technique to map the translatomes of excitatory glutamatergic (vGluT2+) and inhibitory GABA and/or glycinergic (vGAT+ or Gad67+) neurons of the mouse spinal cord. Our analyses demonstrate that inhibitory and excitatory neurons are not only set apart, as expected, by the expression of genes related to the production, release or re-uptake of their principal neurotransmitters and by genes encoding for transcription factors, but also by a differential engagement of neuromodulator, especially neuropeptide, signaling pathways. Subsequent multiplex in situ hybridization revealed eleven neuropeptide genes that are strongly enriched in excitatory dorsal horn neurons and display largely non-overlapping expression patterns closely adhering to the laminar and presumably also functional organization of the spinal cord grey matter.Rebecca Rani Das GuptaLouis ScheurerPawel PelczarHendrik WildnerHanns Ulrich ZeilhoferNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rebecca Rani Das Gupta Louis Scheurer Pawel Pelczar Hendrik Wildner Hanns Ulrich Zeilhofer Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
description	Abstract The spinal dorsal horn harbors a sophisticated and heterogeneous network of excitatory and inhibitory neurons that process peripheral signals encoding different sensory modalities. Although it has long been recognized that this network is crucial both for the separation and the integration of sensory signals of different modalities, a systematic unbiased approach to the use of specific neuromodulatory systems is still missing. Here, we have used the translating ribosome affinity purification (TRAP) technique to map the translatomes of excitatory glutamatergic (vGluT2+) and inhibitory GABA and/or glycinergic (vGAT+ or Gad67+) neurons of the mouse spinal cord. Our analyses demonstrate that inhibitory and excitatory neurons are not only set apart, as expected, by the expression of genes related to the production, release or re-uptake of their principal neurotransmitters and by genes encoding for transcription factors, but also by a differential engagement of neuromodulator, especially neuropeptide, signaling pathways. Subsequent multiplex in situ hybridization revealed eleven neuropeptide genes that are strongly enriched in excitatory dorsal horn neurons and display largely non-overlapping expression patterns closely adhering to the laminar and presumably also functional organization of the spinal cord grey matter.
format	article
author	Rebecca Rani Das Gupta Louis Scheurer Pawel Pelczar Hendrik Wildner Hanns Ulrich Zeilhofer
author_facet	Rebecca Rani Das Gupta Louis Scheurer Pawel Pelczar Hendrik Wildner Hanns Ulrich Zeilhofer
author_sort	Rebecca Rani Das Gupta
title	Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
title_short	Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
title_full	Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
title_fullStr	Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
title_full_unstemmed	Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
title_sort	neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7ed2f83328d34b1d85c69a4326b33081
work_keys_str_mv	AT rebeccaranidasgupta neuronspecificspinalcordtranslatomesrevealaneuropeptidecodeformousedorsalhornexcitatoryneurons AT louisscheurer neuronspecificspinalcordtranslatomesrevealaneuropeptidecodeformousedorsalhornexcitatoryneurons AT pawelpelczar neuronspecificspinalcordtranslatomesrevealaneuropeptidecodeformousedorsalhornexcitatoryneurons AT hendrikwildner neuronspecificspinalcordtranslatomesrevealaneuropeptidecodeformousedorsalhornexcitatoryneurons AT hannsulrichzeilhofer neuronspecificspinalcordtranslatomesrevealaneuropeptidecodeformousedorsalhornexcitatoryneurons
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Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons

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