Self-assembled polymeric nanoparticles as new, smart contrast agents for cancer early detection using magnetic resonance imaging

Fouzi Mouffouk,1,* Teresa Simão,2,* Daniel F Dornelles,2 André D Lopes,3 Pablo Sau,4 Jorge Martins,2,5 Khalid M Abu-Salah,6 Salman A Alrokayan,6 Ana M Rosa da Costa,3 Nuno R dos Santos2 1Chemistry Department, Faculty of Science, Kuwait University, Safat, Kuwait; 2IBB &nda...

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Autores principales:	Mouffouk F, Simão T, Dornelles DF, Lopes AD, Sau P, Martins J, Abu-Salah KM, Alrokayan SA, Rosa da Costa AM, dos Santos NR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7ed39c771068473fb564ae5a76592a45
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spelling	oai:doaj.org-article:7ed39c771068473fb564ae5a76592a452021-12-02T04:05:36ZSelf-assembled polymeric nanoparticles as new, smart contrast agents for cancer early detection using magnetic resonance imaging1178-2013https://doaj.org/article/7ed39c771068473fb564ae5a76592a452014-12-01T00:00:00Zhttp://www.dovepress.com/self-assembled-polymeric-nanoparticles-as-new-smart-contrast-agents-fo-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Fouzi Mouffouk,1,* Teresa Simão,2,* Daniel F Dornelles,2 André D Lopes,3 Pablo Sau,4 Jorge Martins,2,5 Khalid M Abu-Salah,6 Salman A Alrokayan,6 Ana M Rosa da Costa,3 Nuno R dos Santos2 1Chemistry Department, Faculty of Science, Kuwait University, Safat, Kuwait; 2IBB – Institute for Biotechnology and Bioengineering, CBME – Centre for Molecular and Structural Biomedicine, 3CIQA-Algarve Chemistry Research Center, Department of Chemistry and Pharmacy, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Faro, Portugal; 4Centro Radiológico Computarizado SA (CERCO), Seville, Spain; 5Department of Biological Sciences and Bioengineering, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Faro, Portugal; 6King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia *These authors contributed equally to this work Abstract: Early cancer detection is a major factor in the reduction of mortality and cancer management cost. Here we developed a smart and targeted micelle-based contrast agent for magnetic resonance imaging (MRI), able to turn on its imaging capability in the presence of acidic cancer tissues. This smart contrast agent consists of pH-sensitive polymeric micelles formed by self-assembly of a diblock copolymer (poly(ethyleneglycol-b-trimethylsilyl methacrylate)), loaded with a gadolinium hydrophobic complex (tBuBipyGd) and exploits the acidic pH in cancer tissues. In vitro MRI experiments showed that tBuBipyGd-loaded micelles were pH-sensitive, as they turned on their imaging capability only in an acidic microenvironment. The micelle-targeting ability toward cancer cells was enhanced by conjugation with an antibody against the MUC1 protein. The ability of our antibody-decorated micelles to be switched on in acidic microenvironments and to target cancer cells expressing specific antigens, together with its high Gd(III) content and its small size (35–40 nm) reveals their potential use for early cancer detection by MRI. Keywords: micelle, pH-sensitive, self-assembly, smart contrast agent, cancer detectionMouffouk FSimão TDornelles DFLopes ADSau PMartins JAbu-Salah KMAlrokayan SARosa da Costa AMdos Santos NRDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 63-76 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Mouffouk F Simão T Dornelles DF Lopes AD Sau P Martins J Abu-Salah KM Alrokayan SA Rosa da Costa AM dos Santos NR Self-assembled polymeric nanoparticles as new, smart contrast agents for cancer early detection using magnetic resonance imaging
description	Fouzi Mouffouk,1,* Teresa Simão,2,* Daniel F Dornelles,2 André D Lopes,3 Pablo Sau,4 Jorge Martins,2,5 Khalid M Abu-Salah,6 Salman A Alrokayan,6 Ana M Rosa da Costa,3 Nuno R dos Santos2 1Chemistry Department, Faculty of Science, Kuwait University, Safat, Kuwait; 2IBB – Institute for Biotechnology and Bioengineering, CBME – Centre for Molecular and Structural Biomedicine, 3CIQA-Algarve Chemistry Research Center, Department of Chemistry and Pharmacy, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Faro, Portugal; 4Centro Radiológico Computarizado SA (CERCO), Seville, Spain; 5Department of Biological Sciences and Bioengineering, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Faro, Portugal; 6King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia *These authors contributed equally to this work Abstract: Early cancer detection is a major factor in the reduction of mortality and cancer management cost. Here we developed a smart and targeted micelle-based contrast agent for magnetic resonance imaging (MRI), able to turn on its imaging capability in the presence of acidic cancer tissues. This smart contrast agent consists of pH-sensitive polymeric micelles formed by self-assembly of a diblock copolymer (poly(ethyleneglycol-b-trimethylsilyl methacrylate)), loaded with a gadolinium hydrophobic complex (tBuBipyGd) and exploits the acidic pH in cancer tissues. In vitro MRI experiments showed that tBuBipyGd-loaded micelles were pH-sensitive, as they turned on their imaging capability only in an acidic microenvironment. The micelle-targeting ability toward cancer cells was enhanced by conjugation with an antibody against the MUC1 protein. The ability of our antibody-decorated micelles to be switched on in acidic microenvironments and to target cancer cells expressing specific antigens, together with its high Gd(III) content and its small size (35–40 nm) reveals their potential use for early cancer detection by MRI. Keywords: micelle, pH-sensitive, self-assembly, smart contrast agent, cancer detection
format	article
author	Mouffouk F Simão T Dornelles DF Lopes AD Sau P Martins J Abu-Salah KM Alrokayan SA Rosa da Costa AM dos Santos NR
author_facet	Mouffouk F Simão T Dornelles DF Lopes AD Sau P Martins J Abu-Salah KM Alrokayan SA Rosa da Costa AM dos Santos NR
author_sort	Mouffouk F
title	Self-assembled polymeric nanoparticles as new, smart contrast agents for cancer early detection using magnetic resonance imaging
title_short	Self-assembled polymeric nanoparticles as new, smart contrast agents for cancer early detection using magnetic resonance imaging
title_full	Self-assembled polymeric nanoparticles as new, smart contrast agents for cancer early detection using magnetic resonance imaging
title_fullStr	Self-assembled polymeric nanoparticles as new, smart contrast agents for cancer early detection using magnetic resonance imaging
title_full_unstemmed	Self-assembled polymeric nanoparticles as new, smart contrast agents for cancer early detection using magnetic resonance imaging
title_sort	self-assembled polymeric nanoparticles as new, smart contrast agents for cancer early detection using magnetic resonance imaging
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/7ed39c771068473fb564ae5a76592a45
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Self-assembled polymeric nanoparticles as new, smart contrast agents for cancer early detection using magnetic resonance imaging

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