Kaposi’s sarcoma-associated herpesvirus ORF34 is essential for late gene expression and virus production

Abstract Kaposi’s sarcoma-associated herpesvirus (KSHV) is the causative agent of Kaposi’s sarcoma, primary effusion lymphoma, and multicentric Castleman’s disease. KSHV establishes a life-long infection in its host and alternates between a latent and lytic infection state. During lytic infection, l...

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Autores principales:	Mayu Nishimura, Tadashi Watanabe, Syota Yagi, Takahiro Yamanaka, Masahiro Fujimuro
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7edb23e7d1dd45b89d061a74f4b21538
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spelling	oai:doaj.org-article:7edb23e7d1dd45b89d061a74f4b215382021-12-02T12:32:16ZKaposi’s sarcoma-associated herpesvirus ORF34 is essential for late gene expression and virus production10.1038/s41598-017-00401-72045-2322https://doaj.org/article/7edb23e7d1dd45b89d061a74f4b215382017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00401-7https://doaj.org/toc/2045-2322Abstract Kaposi’s sarcoma-associated herpesvirus (KSHV) is the causative agent of Kaposi’s sarcoma, primary effusion lymphoma, and multicentric Castleman’s disease. KSHV establishes a life-long infection in its host and alternates between a latent and lytic infection state. During lytic infection, lytic-related genes are expressed in a temporal manner and categorized as immediate early, early, and late gene transcripts. ORF34 is an early-late gene that interacts with several viral transcription-associated factors, however its physiological importance remains poorly understood. Here, we investigated the role of ORF34 during KSHV infection by generating ORF34-deficient KSHV, using a bacterial artificial chromosome system. Our results reveal that ORF34-deficient KSHV exhibited significantly attenuated late gene expression and viral production but did not affect viral DNA replication. ORF34 interacted with transcription factors ORF18, ORF24, ORF31, and ORF66, and a novel ORF34-interaction partner, ORF23. The C-terminal region of ORF34 was important for interaction with ORF24 and viral production. Our data support a model, in which ORF34 serves as a hub for recruiting a viral transcription complex to ORF24 to promote late viral gene expression.Mayu NishimuraTadashi WatanabeSyota YagiTakahiro YamanakaMasahiro FujimuroNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mayu Nishimura Tadashi Watanabe Syota Yagi Takahiro Yamanaka Masahiro Fujimuro Kaposi’s sarcoma-associated herpesvirus ORF34 is essential for late gene expression and virus production
description	Abstract Kaposi’s sarcoma-associated herpesvirus (KSHV) is the causative agent of Kaposi’s sarcoma, primary effusion lymphoma, and multicentric Castleman’s disease. KSHV establishes a life-long infection in its host and alternates between a latent and lytic infection state. During lytic infection, lytic-related genes are expressed in a temporal manner and categorized as immediate early, early, and late gene transcripts. ORF34 is an early-late gene that interacts with several viral transcription-associated factors, however its physiological importance remains poorly understood. Here, we investigated the role of ORF34 during KSHV infection by generating ORF34-deficient KSHV, using a bacterial artificial chromosome system. Our results reveal that ORF34-deficient KSHV exhibited significantly attenuated late gene expression and viral production but did not affect viral DNA replication. ORF34 interacted with transcription factors ORF18, ORF24, ORF31, and ORF66, and a novel ORF34-interaction partner, ORF23. The C-terminal region of ORF34 was important for interaction with ORF24 and viral production. Our data support a model, in which ORF34 serves as a hub for recruiting a viral transcription complex to ORF24 to promote late viral gene expression.
format	article
author	Mayu Nishimura Tadashi Watanabe Syota Yagi Takahiro Yamanaka Masahiro Fujimuro
author_facet	Mayu Nishimura Tadashi Watanabe Syota Yagi Takahiro Yamanaka Masahiro Fujimuro
author_sort	Mayu Nishimura
title	Kaposi’s sarcoma-associated herpesvirus ORF34 is essential for late gene expression and virus production
title_short	Kaposi’s sarcoma-associated herpesvirus ORF34 is essential for late gene expression and virus production
title_full	Kaposi’s sarcoma-associated herpesvirus ORF34 is essential for late gene expression and virus production
title_fullStr	Kaposi’s sarcoma-associated herpesvirus ORF34 is essential for late gene expression and virus production
title_full_unstemmed	Kaposi’s sarcoma-associated herpesvirus ORF34 is essential for late gene expression and virus production
title_sort	kaposi’s sarcoma-associated herpesvirus orf34 is essential for late gene expression and virus production
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7edb23e7d1dd45b89d061a74f4b21538
work_keys_str_mv	AT mayunishimura kaposissarcomaassociatedherpesvirusorf34isessentialforlategeneexpressionandvirusproduction AT tadashiwatanabe kaposissarcomaassociatedherpesvirusorf34isessentialforlategeneexpressionandvirusproduction AT syotayagi kaposissarcomaassociatedherpesvirusorf34isessentialforlategeneexpressionandvirusproduction AT takahiroyamanaka kaposissarcomaassociatedherpesvirusorf34isessentialforlategeneexpressionandvirusproduction AT masahirofujimuro kaposissarcomaassociatedherpesvirusorf34isessentialforlategeneexpressionandvirusproduction
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Kaposi’s sarcoma-associated herpesvirus ORF34 is essential for late gene expression and virus production

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