Snail1 protein in the stroma as a new putative prognosis marker for colon tumours.

Over-expression of Snail1 gene transcriptional repressor promotes an epithelial-to-mesenchymal transition in epithelial tumour cell lines. Expression of Snail1 RNA has been associated to the pathogenesis of a number of malignancies; however, the lack of good monoclonal antibodies against this protei...

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Autores principales:	Clara Francí, Manel Gallén, Francesc Alameda, Teresa Baró, Mar Iglesias, Ismo Virtanen, Antonio García de Herreros
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7edd08d706e3486488044ff0fd1e97fb
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spelling	oai:doaj.org-article:7edd08d706e3486488044ff0fd1e97fb2021-11-25T06:22:38ZSnail1 protein in the stroma as a new putative prognosis marker for colon tumours.1932-620310.1371/journal.pone.0005595https://doaj.org/article/7edd08d706e3486488044ff0fd1e97fb2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19440385/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Over-expression of Snail1 gene transcriptional repressor promotes an epithelial-to-mesenchymal transition in epithelial tumour cell lines. Expression of Snail1 RNA has been associated to the pathogenesis of a number of malignancies; however, the lack of good monoclonal antibodies against this protein has precluded a definitive analysis of Snail1 protein. In this study, we aimed to determine the expression of this transcriptional factor in colorectal tumours. Using a Snail1 well-characterized monoclonal antibody developed in our laboratories we have analyzed by immunohistochemistry a cohort of 162 human colorectal tumours. Ninety tumours (56%) showed nuclear expression in the tumoral tissue and the adjacent stroma; in 34 (21%), Snail1 was detected just in the stroma, whereas in only 4 the expression of Snail1 was detected in the tumoral tissue and the stroma was negative. No correlation was found between the presence of Snail1 in the tumour and tumour stage; however, a trend (p = 0.054) was detected when the expression of this factor in the stroma was considered. Snail1 immunoreactivity in this compartment was associated with presence of distant metastasis (p = 0.006). Moreover, expression of Snail1 in the tumor stroma correlated with lower specific survival of cancer patients (p = 0.011). Interestingly, this correlation was also detected in stage I and II tumors. Therefore, our results indicate that the presence of nuclear Snail1 immunoreactive cells in the stroma may be an informative indicator of prognosis of colon tumours especially useful in those corresponding to lower stages and identify a new marker suitable to label activated stroma in colon tumours.Clara FrancíManel GallénFrancesc AlamedaTeresa BaróMar IglesiasIsmo VirtanenAntonio García de HerrerosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 5, p e5595 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Clara Francí Manel Gallén Francesc Alameda Teresa Baró Mar Iglesias Ismo Virtanen Antonio García de Herreros Snail1 protein in the stroma as a new putative prognosis marker for colon tumours.
description	Over-expression of Snail1 gene transcriptional repressor promotes an epithelial-to-mesenchymal transition in epithelial tumour cell lines. Expression of Snail1 RNA has been associated to the pathogenesis of a number of malignancies; however, the lack of good monoclonal antibodies against this protein has precluded a definitive analysis of Snail1 protein. In this study, we aimed to determine the expression of this transcriptional factor in colorectal tumours. Using a Snail1 well-characterized monoclonal antibody developed in our laboratories we have analyzed by immunohistochemistry a cohort of 162 human colorectal tumours. Ninety tumours (56%) showed nuclear expression in the tumoral tissue and the adjacent stroma; in 34 (21%), Snail1 was detected just in the stroma, whereas in only 4 the expression of Snail1 was detected in the tumoral tissue and the stroma was negative. No correlation was found between the presence of Snail1 in the tumour and tumour stage; however, a trend (p = 0.054) was detected when the expression of this factor in the stroma was considered. Snail1 immunoreactivity in this compartment was associated with presence of distant metastasis (p = 0.006). Moreover, expression of Snail1 in the tumor stroma correlated with lower specific survival of cancer patients (p = 0.011). Interestingly, this correlation was also detected in stage I and II tumors. Therefore, our results indicate that the presence of nuclear Snail1 immunoreactive cells in the stroma may be an informative indicator of prognosis of colon tumours especially useful in those corresponding to lower stages and identify a new marker suitable to label activated stroma in colon tumours.
format	article
author	Clara Francí Manel Gallén Francesc Alameda Teresa Baró Mar Iglesias Ismo Virtanen Antonio García de Herreros
author_facet	Clara Francí Manel Gallén Francesc Alameda Teresa Baró Mar Iglesias Ismo Virtanen Antonio García de Herreros
author_sort	Clara Francí
title	Snail1 protein in the stroma as a new putative prognosis marker for colon tumours.
title_short	Snail1 protein in the stroma as a new putative prognosis marker for colon tumours.
title_full	Snail1 protein in the stroma as a new putative prognosis marker for colon tumours.
title_fullStr	Snail1 protein in the stroma as a new putative prognosis marker for colon tumours.
title_full_unstemmed	Snail1 protein in the stroma as a new putative prognosis marker for colon tumours.
title_sort	snail1 protein in the stroma as a new putative prognosis marker for colon tumours.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/7edd08d706e3486488044ff0fd1e97fb
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Snail1 protein in the stroma as a new putative prognosis marker for colon tumours.

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