Age-Related Changes in Lipidome of Rat Frontal Cortex and Cerebellum Are Partially Reversed by Methionine Restriction Applied in Old Age

Lipids are closely associated with brain structure and function. However, the potential changes in the lipidome induced by aging remain to be elucidated. In this study, we used chromatographic techniques and a mass spectrometry-based approach to evaluate age-associated changes in the lipidome of the...

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Autores principales:	Mariona Jové, Rosanna Cabré, Natàlia Mota-Martorell, Meritxell Martin-Garí, Èlia Obis, Paula Ramos, Iván Canales, José Daniel Galo-Licona, Joaquim Sol, Lara Nogueras, Pascual Torres, Manuel Portero-Otín, Victòria Ayala, Isidro Ferrer, Reinald Pamplona
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	aging glycerolipids glycerophospholipids (lip)oxidation-derived protein damage markers mass spectrometry methionine restriction Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/7ee6724b919c4717b9a470ea865bed17
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spelling	oai:doaj.org-article:7ee6724b919c4717b9a470ea865bed172021-11-25T17:57:27ZAge-Related Changes in Lipidome of Rat Frontal Cortex and Cerebellum Are Partially Reversed by Methionine Restriction Applied in Old Age10.3390/ijms2222125171422-00671661-6596https://doaj.org/article/7ee6724b919c4717b9a470ea865bed172021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12517https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Lipids are closely associated with brain structure and function. However, the potential changes in the lipidome induced by aging remain to be elucidated. In this study, we used chromatographic techniques and a mass spectrometry-based approach to evaluate age-associated changes in the lipidome of the frontal cortex and cerebellum obtained from adult male Wistar rats (8 months), aged male Wistar rats (26 months), and aged male Wistar rats submitted to a methionine restriction diet (MetR)—as an anti-aging intervention—for 8 weeks. The outcomes revealed that only small changes (about 10%) were observed in the lipidome profile in the cerebellum and frontal cortex during aging, and these changes differed, in some cases, between regions. Furthermore, a MetR diet partially reversed the effects of the aging process. Remarkably, the most affected lipid classes were ether-triacylglycerols, diacylglycerols, phosphatidylethanolamine N-methylated, plasmalogens, ceramides, and cholesterol esters. When the fatty acid profile was analyzed, we observed that the frontal cortex is highly preserved during aging and maintained under MetR, whereas in the cerebellum minor changes (increased monounsaturated and decreased polyunsaturated contents) were observed and not reversed by MetR. We conclude that the rat cerebellum and frontal cortex have efficient mechanisms to preserve the lipid profile of their cell membranes throughout their adult lifespan in order to maintain brain structure and function. A part of the small changes that take place during aging can be reversed with a MetR diet applied in old age.Mariona JovéRosanna CabréNatàlia Mota-MartorellMeritxell Martin-GaríÈlia ObisPaula RamosIván CanalesJosé Daniel Galo-LiconaJoaquim SolLara NoguerasPascual TorresManuel Portero-OtínVictòria AyalaIsidro FerrerReinald PamplonaMDPI AGarticleagingglycerolipidsglycerophospholipids(lip)oxidation-derived protein damage markersmass spectrometrymethionine restrictionBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12517, p 12517 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aging glycerolipids glycerophospholipids (lip)oxidation-derived protein damage markers mass spectrometry methionine restriction Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	aging glycerolipids glycerophospholipids (lip)oxidation-derived protein damage markers mass spectrometry methionine restriction Biology (General) QH301-705.5 Chemistry QD1-999 Mariona Jové Rosanna Cabré Natàlia Mota-Martorell Meritxell Martin-Garí Èlia Obis Paula Ramos Iván Canales José Daniel Galo-Licona Joaquim Sol Lara Nogueras Pascual Torres Manuel Portero-Otín Victòria Ayala Isidro Ferrer Reinald Pamplona Age-Related Changes in Lipidome of Rat Frontal Cortex and Cerebellum Are Partially Reversed by Methionine Restriction Applied in Old Age
description	Lipids are closely associated with brain structure and function. However, the potential changes in the lipidome induced by aging remain to be elucidated. In this study, we used chromatographic techniques and a mass spectrometry-based approach to evaluate age-associated changes in the lipidome of the frontal cortex and cerebellum obtained from adult male Wistar rats (8 months), aged male Wistar rats (26 months), and aged male Wistar rats submitted to a methionine restriction diet (MetR)—as an anti-aging intervention—for 8 weeks. The outcomes revealed that only small changes (about 10%) were observed in the lipidome profile in the cerebellum and frontal cortex during aging, and these changes differed, in some cases, between regions. Furthermore, a MetR diet partially reversed the effects of the aging process. Remarkably, the most affected lipid classes were ether-triacylglycerols, diacylglycerols, phosphatidylethanolamine N-methylated, plasmalogens, ceramides, and cholesterol esters. When the fatty acid profile was analyzed, we observed that the frontal cortex is highly preserved during aging and maintained under MetR, whereas in the cerebellum minor changes (increased monounsaturated and decreased polyunsaturated contents) were observed and not reversed by MetR. We conclude that the rat cerebellum and frontal cortex have efficient mechanisms to preserve the lipid profile of their cell membranes throughout their adult lifespan in order to maintain brain structure and function. A part of the small changes that take place during aging can be reversed with a MetR diet applied in old age.
format	article
author	Mariona Jové Rosanna Cabré Natàlia Mota-Martorell Meritxell Martin-Garí Èlia Obis Paula Ramos Iván Canales José Daniel Galo-Licona Joaquim Sol Lara Nogueras Pascual Torres Manuel Portero-Otín Victòria Ayala Isidro Ferrer Reinald Pamplona
author_facet	Mariona Jové Rosanna Cabré Natàlia Mota-Martorell Meritxell Martin-Garí Èlia Obis Paula Ramos Iván Canales José Daniel Galo-Licona Joaquim Sol Lara Nogueras Pascual Torres Manuel Portero-Otín Victòria Ayala Isidro Ferrer Reinald Pamplona
author_sort	Mariona Jové
title	Age-Related Changes in Lipidome of Rat Frontal Cortex and Cerebellum Are Partially Reversed by Methionine Restriction Applied in Old Age
title_short	Age-Related Changes in Lipidome of Rat Frontal Cortex and Cerebellum Are Partially Reversed by Methionine Restriction Applied in Old Age
title_full	Age-Related Changes in Lipidome of Rat Frontal Cortex and Cerebellum Are Partially Reversed by Methionine Restriction Applied in Old Age
title_fullStr	Age-Related Changes in Lipidome of Rat Frontal Cortex and Cerebellum Are Partially Reversed by Methionine Restriction Applied in Old Age
title_full_unstemmed	Age-Related Changes in Lipidome of Rat Frontal Cortex and Cerebellum Are Partially Reversed by Methionine Restriction Applied in Old Age
title_sort	age-related changes in lipidome of rat frontal cortex and cerebellum are partially reversed by methionine restriction applied in old age
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7ee6724b919c4717b9a470ea865bed17
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Age-Related Changes in Lipidome of Rat Frontal Cortex and Cerebellum Are Partially Reversed by Methionine Restriction Applied in Old Age

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