Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules

Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules

Recombinant MHC class II molecules are instrumental in antigen-specific T-cell identification assays and showed efficacy as experimental medicines. Here, the authors engineer MHC class II molecules with species-specific knob-into-hole heteromerization domains, enabling a translatable purification pr...

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Autores principales: Pau Serra, Nahir Garabatos, Santiswarup Singha, César Fandos, Josep Garnica, Patricia Solé, Daniel Parras, Jun Yamanouchi, Jesús Blanco, Meritxell Tort, Mireia Ortega, Yang Yang, Kristofor K. Ellestad, Pere Santamaria
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Science
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Acceso en línea:https://doaj.org/article/7ee67d52977841a9b1785048df8248f6
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Sumario:Recombinant MHC class II molecules are instrumental in antigen-specific T-cell identification assays and showed efficacy as experimental medicines. Here, the authors engineer MHC class II molecules with species-specific knob-into-hole heteromerization domains, enabling a translatable purification process with improved stability, yields, and biological potency.

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