Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules

Recombinant MHC class II molecules are instrumental in antigen-specific T-cell identification assays and showed efficacy as experimental medicines. Here, the authors engineer MHC class II molecules with species-specific knob-into-hole heteromerization domains, enabling a translatable purification pr...

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Autores principales:	Pau Serra, Nahir Garabatos, Santiswarup Singha, César Fandos, Josep Garnica, Patricia Solé, Daniel Parras, Jun Yamanouchi, Jesús Blanco, Meritxell Tort, Mireia Ortega, Yang Yang, Kristofor K. Ellestad, Pere Santamaria
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/7ee67d52977841a9b1785048df8248f6
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spelling	oai:doaj.org-article:7ee67d52977841a9b1785048df8248f62021-12-02T15:35:34ZIncreased yields and biological potency of knob-into-hole-based soluble MHC class II molecules10.1038/s41467-019-12902-22041-1723https://doaj.org/article/7ee67d52977841a9b1785048df8248f62019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12902-2https://doaj.org/toc/2041-1723Recombinant MHC class II molecules are instrumental in antigen-specific T-cell identification assays and showed efficacy as experimental medicines. Here, the authors engineer MHC class II molecules with species-specific knob-into-hole heteromerization domains, enabling a translatable purification process with improved stability, yields, and biological potency.Pau SerraNahir GarabatosSantiswarup SinghaCésar FandosJosep GarnicaPatricia SoléDaniel ParrasJun YamanouchiJesús BlancoMeritxell TortMireia OrtegaYang YangKristofor K. EllestadPere SantamariaNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Pau Serra Nahir Garabatos Santiswarup Singha César Fandos Josep Garnica Patricia Solé Daniel Parras Jun Yamanouchi Jesús Blanco Meritxell Tort Mireia Ortega Yang Yang Kristofor K. Ellestad Pere Santamaria Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules
description	Recombinant MHC class II molecules are instrumental in antigen-specific T-cell identification assays and showed efficacy as experimental medicines. Here, the authors engineer MHC class II molecules with species-specific knob-into-hole heteromerization domains, enabling a translatable purification process with improved stability, yields, and biological potency.
format	article
author	Pau Serra Nahir Garabatos Santiswarup Singha César Fandos Josep Garnica Patricia Solé Daniel Parras Jun Yamanouchi Jesús Blanco Meritxell Tort Mireia Ortega Yang Yang Kristofor K. Ellestad Pere Santamaria
author_facet	Pau Serra Nahir Garabatos Santiswarup Singha César Fandos Josep Garnica Patricia Solé Daniel Parras Jun Yamanouchi Jesús Blanco Meritxell Tort Mireia Ortega Yang Yang Kristofor K. Ellestad Pere Santamaria
author_sort	Pau Serra
title	Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules
title_short	Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules
title_full	Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules
title_fullStr	Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules
title_full_unstemmed	Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules
title_sort	increased yields and biological potency of knob-into-hole-based soluble mhc class ii molecules
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/7ee67d52977841a9b1785048df8248f6
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Increased yields and biological potency of knob-into-hole-based soluble MHC class II molecules

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