Undifferentiated In Vitro Cultured <i>Actinidia deliciosa</i> as Cell Factory for the Production of Quercetin Glycosides

Land plants produce a vast arsenal of specialized metabolites and many of them display interesting bioactivities in humans. Recently, flavonol quercetin gained great attention in the light of the COVID-19 pandemic because, in addition to the anti-inflammatory, antiviral and anti-cancer activity alre...

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Autores principales:	Stefano Negri, Sofia Gambini, Stefania Ceoldo, Linda Avesani, Mauro Commisso, Flavia Guzzo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	quercetin flavonoids plant cell and tissue culture <i>Actinida deliciosa</i> <i>Actinidia chinensis</i> Botany QK1-989
Acceso en línea:	https://doaj.org/article/7ee8610687164462ab1390fab1518eda
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spelling	oai:doaj.org-article:7ee8610687164462ab1390fab1518eda2021-11-25T18:47:06ZUndifferentiated In Vitro Cultured <i>Actinidia deliciosa</i> as Cell Factory for the Production of Quercetin Glycosides10.3390/plants101124992223-7747https://doaj.org/article/7ee8610687164462ab1390fab1518eda2021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2499https://doaj.org/toc/2223-7747Land plants produce a vast arsenal of specialized metabolites and many of them display interesting bioactivities in humans. Recently, flavonol quercetin gained great attention in the light of the COVID-19 pandemic because, in addition to the anti-inflammatory, antiviral and anti-cancer activity already described, it emerged as possible inhibitor of 3CLpro, the major protease of SARS-CoV-2 virus. Plant cell and tissue culture (PCTC) is an attractive platform for the biotechnological production of plant metabolites. This technology allows a large amount of water and agricultural land to be saved and, being free of contaminants in the process, it is suitable for scaling up the production in bioreactors. In a project aimed to generate and screen in vitro plant cells for the production of valuable specialized metabolites for commercial production, we generated various cell lines from <i>Actinidia deliciosa</i> (kiwi fruit tree) and <i>Actinidia chinensis</i> (gold kiwi fruit tree), that were able to produce relevant amounts of quercetin derivatives, mainly quercetin glycosides. Three cell lines from <i>A. deliciosa</i> were characterized by targeted and untargeted metabolomics. In standard growing conditions, they produce and accumulate up to 13.26 mg/100 g fresh weight (419.76 mg/100 g dry weight) of quercetin derivatives. To address future industrial applications, these cell lines should be entered into an acceleration program to further increase the amount of these metabolites by optimizing the culture conditions and elicitation.Stefano NegriSofia GambiniStefania CeoldoLinda AvesaniMauro CommissoFlavia GuzzoMDPI AGarticlequercetinflavonoidsplant cell and tissue culture<i>Actinida deliciosa</i><i>Actinidia chinensis</i>BotanyQK1-989ENPlants, Vol 10, Iss 2499, p 2499 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	quercetin flavonoids plant cell and tissue culture <i>Actinida deliciosa</i> <i>Actinidia chinensis</i> Botany QK1-989
spellingShingle	quercetin flavonoids plant cell and tissue culture <i>Actinida deliciosa</i> <i>Actinidia chinensis</i> Botany QK1-989 Stefano Negri Sofia Gambini Stefania Ceoldo Linda Avesani Mauro Commisso Flavia Guzzo Undifferentiated In Vitro Cultured <i>Actinidia deliciosa</i> as Cell Factory for the Production of Quercetin Glycosides
description	Land plants produce a vast arsenal of specialized metabolites and many of them display interesting bioactivities in humans. Recently, flavonol quercetin gained great attention in the light of the COVID-19 pandemic because, in addition to the anti-inflammatory, antiviral and anti-cancer activity already described, it emerged as possible inhibitor of 3CLpro, the major protease of SARS-CoV-2 virus. Plant cell and tissue culture (PCTC) is an attractive platform for the biotechnological production of plant metabolites. This technology allows a large amount of water and agricultural land to be saved and, being free of contaminants in the process, it is suitable for scaling up the production in bioreactors. In a project aimed to generate and screen in vitro plant cells for the production of valuable specialized metabolites for commercial production, we generated various cell lines from <i>Actinidia deliciosa</i> (kiwi fruit tree) and <i>Actinidia chinensis</i> (gold kiwi fruit tree), that were able to produce relevant amounts of quercetin derivatives, mainly quercetin glycosides. Three cell lines from <i>A. deliciosa</i> were characterized by targeted and untargeted metabolomics. In standard growing conditions, they produce and accumulate up to 13.26 mg/100 g fresh weight (419.76 mg/100 g dry weight) of quercetin derivatives. To address future industrial applications, these cell lines should be entered into an acceleration program to further increase the amount of these metabolites by optimizing the culture conditions and elicitation.
format	article
author	Stefano Negri Sofia Gambini Stefania Ceoldo Linda Avesani Mauro Commisso Flavia Guzzo
author_facet	Stefano Negri Sofia Gambini Stefania Ceoldo Linda Avesani Mauro Commisso Flavia Guzzo
author_sort	Stefano Negri
title	Undifferentiated In Vitro Cultured <i>Actinidia deliciosa</i> as Cell Factory for the Production of Quercetin Glycosides
title_short	Undifferentiated In Vitro Cultured <i>Actinidia deliciosa</i> as Cell Factory for the Production of Quercetin Glycosides
title_full	Undifferentiated In Vitro Cultured <i>Actinidia deliciosa</i> as Cell Factory for the Production of Quercetin Glycosides
title_fullStr	Undifferentiated In Vitro Cultured <i>Actinidia deliciosa</i> as Cell Factory for the Production of Quercetin Glycosides
title_full_unstemmed	Undifferentiated In Vitro Cultured <i>Actinidia deliciosa</i> as Cell Factory for the Production of Quercetin Glycosides
title_sort	undifferentiated in vitro cultured <i>actinidia deliciosa</i> as cell factory for the production of quercetin glycosides
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7ee8610687164462ab1390fab1518eda
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Undifferentiated In Vitro Cultured <i>Actinidia deliciosa</i> as Cell Factory for the Production of Quercetin Glycosides

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