Composition and acquisition of the microbiome in solitary, ground-nesting alkali bees

Abstract Increasing evidence suggests the microbiome plays an important role in bee ecology and health. However, the relationship between bees and their bacterial symbionts has only been explored in a handful of species. We characterized the microbiome across the life cycle of solitary, ground-nesti...

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Autores principales:	Karen M. Kapheim, Makenna M. Johnson, Maggi Jolley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7efa4e7fd962479ab9ce13336edb008c
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spelling	oai:doaj.org-article:7efa4e7fd962479ab9ce13336edb008c2021-12-02T14:06:56ZComposition and acquisition of the microbiome in solitary, ground-nesting alkali bees10.1038/s41598-021-82573-x2045-2322https://doaj.org/article/7efa4e7fd962479ab9ce13336edb008c2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82573-xhttps://doaj.org/toc/2045-2322Abstract Increasing evidence suggests the microbiome plays an important role in bee ecology and health. However, the relationship between bees and their bacterial symbionts has only been explored in a handful of species. We characterized the microbiome across the life cycle of solitary, ground-nesting alkali bees (Nomia melanderi). We find that feeding status is a major determinant of microbiome composition. The microbiome of feeding larvae was similar to that of pollen provisions, but the microbiome of post-feeding larvae (pre-pupae) was similar to that of the brood cell walls and newly-emerged females. Feeding larvae and pollen provisions had the lowest beta diversity, suggesting the composition of larval diet is highly uniform. Comparisons between lab-reared, newly-emerged, and nesting adult females suggest that the hindgut bacterial community is largely shaped by the external environment. However, we also identified taxa that are likely acquired in the nest or which increase or decrease in relative abundance with age. Although Lactobacillus micheneri was highly prevalent in pollen provisions, it was only detected in one lab-reared female, suggesting it is primarily acquired from environmental sources. These results provide the foundation for future research on metagenomic function and development of probiotics for these native pollinators.Karen M. KapheimMakenna M. JohnsonMaggi JolleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karen M. Kapheim Makenna M. Johnson Maggi Jolley Composition and acquisition of the microbiome in solitary, ground-nesting alkali bees
description	Abstract Increasing evidence suggests the microbiome plays an important role in bee ecology and health. However, the relationship between bees and their bacterial symbionts has only been explored in a handful of species. We characterized the microbiome across the life cycle of solitary, ground-nesting alkali bees (Nomia melanderi). We find that feeding status is a major determinant of microbiome composition. The microbiome of feeding larvae was similar to that of pollen provisions, but the microbiome of post-feeding larvae (pre-pupae) was similar to that of the brood cell walls and newly-emerged females. Feeding larvae and pollen provisions had the lowest beta diversity, suggesting the composition of larval diet is highly uniform. Comparisons between lab-reared, newly-emerged, and nesting adult females suggest that the hindgut bacterial community is largely shaped by the external environment. However, we also identified taxa that are likely acquired in the nest or which increase or decrease in relative abundance with age. Although Lactobacillus micheneri was highly prevalent in pollen provisions, it was only detected in one lab-reared female, suggesting it is primarily acquired from environmental sources. These results provide the foundation for future research on metagenomic function and development of probiotics for these native pollinators.
format	article
author	Karen M. Kapheim Makenna M. Johnson Maggi Jolley
author_facet	Karen M. Kapheim Makenna M. Johnson Maggi Jolley
author_sort	Karen M. Kapheim
title	Composition and acquisition of the microbiome in solitary, ground-nesting alkali bees
title_short	Composition and acquisition of the microbiome in solitary, ground-nesting alkali bees
title_full	Composition and acquisition of the microbiome in solitary, ground-nesting alkali bees
title_fullStr	Composition and acquisition of the microbiome in solitary, ground-nesting alkali bees
title_full_unstemmed	Composition and acquisition of the microbiome in solitary, ground-nesting alkali bees
title_sort	composition and acquisition of the microbiome in solitary, ground-nesting alkali bees
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7efa4e7fd962479ab9ce13336edb008c
work_keys_str_mv	AT karenmkapheim compositionandacquisitionofthemicrobiomeinsolitarygroundnestingalkalibees AT makennamjohnson compositionandacquisitionofthemicrobiomeinsolitarygroundnestingalkalibees AT maggijolley compositionandacquisitionofthemicrobiomeinsolitarygroundnestingalkalibees
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Composition and acquisition of the microbiome in solitary, ground-nesting alkali bees

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