Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis

Angelino Julio Cariello,1 Paulo Jos&eacute; Martins Bispo,2 Gabriela Freitas Pereira de Souza,3 Antonio Carlos Campos Pignatari,2 Marcelo Ganzarolli de Oliveira,3 Ana Luisa Hofling-Lima11Department of Ophthalmology, 2Division of Infectious Diseases, Federal University of S&atilde;o P...

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Autores principales:	de Oliveira MG, Pignatari AC, de Souza GF, Bispo PJ, Cariello AJ, Hofling-Lima AL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/7efc2496812f4d8288fb0ce70664dad1
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spelling	oai:doaj.org-article:7efc2496812f4d8288fb0ce70664dad12021-12-02T09:09:07ZBactericidal effect of S-nitrosothiols against clinical isolates from keratitis1177-54671177-5483https://doaj.org/article/7efc2496812f4d8288fb0ce70664dad12012-11-01T00:00:00Zhttp://www.dovepress.com/bactericidal-effect-of-s-nitrosothiols-against-clinical-isolates-from--a11558https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Angelino Julio Cariello,1 Paulo Jos&eacute; Martins Bispo,2 Gabriela Freitas Pereira de Souza,3 Antonio Carlos Campos Pignatari,2 Marcelo Ganzarolli de Oliveira,3 Ana Luisa Hofling-Lima11Department of Ophthalmology, 2Division of Infectious Diseases, Federal University of S&atilde;o Paulo, 3Institute of Chemistry, University of Campinas, Campinas, S&atilde;o Paulo, BrazilBackground: The purpose of this study was to evaluate the antimicrobial activity of two nitric oxide donors, ie, S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylcysteine (SNAC), against clinical isolates from patients with infectious keratitis.Methods: Reference broth microdilution assays were performed to determine the minimum inhibitory and bactericidal concentrations for GSNO and SNAC against four American Type Culture Collection strains and 52 clinical isolates from patients with infectious keratitis as follows: 14 (26.9%) Pseudomonas species; 13 (25.0%) coagulase-negative Staphylococci; 10 (19.2%) Staphylococcus aureus; nine (17.3%) Serratia marcescens; and six (11.5%) Enterobacter aerogenes. Sterility control and bacterial growth control were also performed.Results: SNAC showed lower minimum inhibitory and bactericidal concentrations than GSNO for all clinical isolates from patients with infectious keratitis. For Gram-positive bacteria, mean minimum inhibitory and bactericidal concentrations were 2.1 &plusmn; 1.3 and 8.6 &plusmn; 3.8 mM for SNAC and 4.6 &plusmn; 3.2 and 21.5 &plusmn; 12.5 mM for GSNO (P &lt; 0.01). For Gram-negative bacteria, mean minimum inhibitory and bactericidal concentrations were 3.3 &plusmn; 1.4 and 6.1 &plusmn; 3.4 mM for SNAC and 12.4 &plusmn; 5.4 and 26.5 &plusmn; 10.1 mM for GSNO (P &lt; 0.01). The minimum bactericidal to inhibitory concentration ratio was &le;8 in 100% of all isolates tested for SNAC and in 94.2% tested for GSNO.Conclusions: SNAC and GSNO had effective inhibitory and bactericidal effects against bacterial isolates from keratitis. SNAC showed greater antimicrobial activity than GSNO against all bacteria. Gram-positive bacteria were more susceptible to the inhibitory and bactericidal effects of the S-nitrosothiols.Keywords: antimicrobial activity, S-nitroso-N-acetylcysteine, S-nitrosoglutathione, nitric oxide donors, infectious keratitisde Oliveira MGPignatari ACde Souza GFBispo PJCariello AJHofling-Lima ALDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2012, Iss default, Pp 1907-1914 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 de Oliveira MG Pignatari AC de Souza GF Bispo PJ Cariello AJ Hofling-Lima AL Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis
description	Angelino Julio Cariello,1 Paulo Jos&eacute; Martins Bispo,2 Gabriela Freitas Pereira de Souza,3 Antonio Carlos Campos Pignatari,2 Marcelo Ganzarolli de Oliveira,3 Ana Luisa Hofling-Lima11Department of Ophthalmology, 2Division of Infectious Diseases, Federal University of S&atilde;o Paulo, 3Institute of Chemistry, University of Campinas, Campinas, S&atilde;o Paulo, BrazilBackground: The purpose of this study was to evaluate the antimicrobial activity of two nitric oxide donors, ie, S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylcysteine (SNAC), against clinical isolates from patients with infectious keratitis.Methods: Reference broth microdilution assays were performed to determine the minimum inhibitory and bactericidal concentrations for GSNO and SNAC against four American Type Culture Collection strains and 52 clinical isolates from patients with infectious keratitis as follows: 14 (26.9%) Pseudomonas species; 13 (25.0%) coagulase-negative Staphylococci; 10 (19.2%) Staphylococcus aureus; nine (17.3%) Serratia marcescens; and six (11.5%) Enterobacter aerogenes. Sterility control and bacterial growth control were also performed.Results: SNAC showed lower minimum inhibitory and bactericidal concentrations than GSNO for all clinical isolates from patients with infectious keratitis. For Gram-positive bacteria, mean minimum inhibitory and bactericidal concentrations were 2.1 &plusmn; 1.3 and 8.6 &plusmn; 3.8 mM for SNAC and 4.6 &plusmn; 3.2 and 21.5 &plusmn; 12.5 mM for GSNO (P &lt; 0.01). For Gram-negative bacteria, mean minimum inhibitory and bactericidal concentrations were 3.3 &plusmn; 1.4 and 6.1 &plusmn; 3.4 mM for SNAC and 12.4 &plusmn; 5.4 and 26.5 &plusmn; 10.1 mM for GSNO (P &lt; 0.01). The minimum bactericidal to inhibitory concentration ratio was &le;8 in 100% of all isolates tested for SNAC and in 94.2% tested for GSNO.Conclusions: SNAC and GSNO had effective inhibitory and bactericidal effects against bacterial isolates from keratitis. SNAC showed greater antimicrobial activity than GSNO against all bacteria. Gram-positive bacteria were more susceptible to the inhibitory and bactericidal effects of the S-nitrosothiols.Keywords: antimicrobial activity, S-nitroso-N-acetylcysteine, S-nitrosoglutathione, nitric oxide donors, infectious keratitis
format	article
author	de Oliveira MG Pignatari AC de Souza GF Bispo PJ Cariello AJ Hofling-Lima AL
author_facet	de Oliveira MG Pignatari AC de Souza GF Bispo PJ Cariello AJ Hofling-Lima AL
author_sort	de Oliveira MG
title	Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis
title_short	Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis
title_full	Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis
title_fullStr	Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis
title_full_unstemmed	Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis
title_sort	bactericidal effect of s-nitrosothiols against clinical isolates from keratitis
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/7efc2496812f4d8288fb0ce70664dad1
work_keys_str_mv	AT deoliveiramg bactericidaleffectofsnitrosothiolsagainstclinicalisolatesfromkeratitis AT pignatariac bactericidaleffectofsnitrosothiolsagainstclinicalisolatesfromkeratitis AT desouzagf bactericidaleffectofsnitrosothiolsagainstclinicalisolatesfromkeratitis AT bispopj bactericidaleffectofsnitrosothiolsagainstclinicalisolatesfromkeratitis AT carielloaj bactericidaleffectofsnitrosothiolsagainstclinicalisolatesfromkeratitis AT hoflinglimaal bactericidaleffectofsnitrosothiolsagainstclinicalisolatesfromkeratitis
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Bactericidal effect of S-nitrosothiols against clinical isolates from keratitis

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