Possible involvement of ROS generation in vorinostat pretreatment induced enhancement of the antibacterial activity of ciprofloxacin

Majed M Masadeh,1 Karem H Alzoubi,2 Sayer I Al-azzam,2 Ahlam M Al-buhairan3 1Department of Pharmaceutical Technology, 2Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid, Jordan; 3Department of Clinical Laboratory Sciences, King Saud University, Riyadh, Saudi Arabia...

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Autores principales:	Masadeh MM, Alzoubi KH, Al-azzam SI, Al-buhairan AM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	ciprofloxacin MIC vorinostat antimicrobial susceptibility Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/7f02025171eb4abfaae32ac8f118642a
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spelling	oai:doaj.org-article:7f02025171eb4abfaae32ac8f118642a2021-12-02T03:26:22ZPossible involvement of ROS generation in vorinostat pretreatment induced enhancement of the antibacterial activity of ciprofloxacin1179-1438https://doaj.org/article/7f02025171eb4abfaae32ac8f118642a2017-10-01T00:00:00Zhttps://www.dovepress.com/possible-involvement-of-ros-generation-in-vorinostat-pretreatment-indu-peer-reviewed-article-CPAAhttps://doaj.org/toc/1179-1438Majed M Masadeh,1 Karem H Alzoubi,2 Sayer I Al-azzam,2 Ahlam M Al-buhairan3 1Department of Pharmaceutical Technology, 2Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid, Jordan; 3Department of Clinical Laboratory Sciences, King Saud University, Riyadh, Saudi Arabia Abstract: The mechanism underlying ciprofloxacin action involves interference with transcription and replication of bacterial DNA and, thus, the induction of double-strand breaks in DNA. It also involves elevated oxidative stress, which might contribute to bacterial cell death. Vorinostat was shown to induce oxidative DNA damage. The current work investigated a possible interactive effect of vorinostat on ciprofloxacin-induced cytotoxicity against a number of reference bacteria. Standard bacterial strains were Escherichia coli ATCC 35218, Staphylococcus aureus ATCC29213, Pseudomonas aeruginosa ATCC 9027, Staphylococcus epidermidis ATCC 12228, Acinetobacter baumannii ATCC 17978, Proteus mirabilis ATCC 12459, Klebsiella pneumoniae ATCC 13883, methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43300), and Streptococcus pneumoniae (ATCC 25923). The antibacterial activity of ciprofloxacin, with or without pretreatment of bacterial cells by vorinostat, was examined using the disc diffusion procedure and determination of the minimum inhibitory concentration (MIC) and zones of inhibition of bacterial growth. All tested bacterial strains showed sensitivity to ciprofloxacin. When pretreated with vorinostat, significantly larger zones of inhibition and smaller MIC values were observed in all bacterial strains compared to those treated with ciprofloxacin alone. In correlation, generation of reactive oxygen species (ROS) induced by the antibacterial action of ciprofloxacin was enhanced by treatment of bacterial cells with vorinostat. Results showed the possible agonistic properties of vorinostat when used together with ciprofloxacin. This could be related to the ability of these agents to enhance oxidative stress in bacterial cells. Keywords: flouroquinolones, MIC, histone deacetylase inhibitor, oxidative stress, antimicrobial susceptibility Masadeh MMAlzoubi KHAl-azzam SIAl-buhairan AMDove Medical PressarticleciprofloxacinMICvorinostatantimicrobial susceptibilityTherapeutics. PharmacologyRM1-950ENClinical Pharmacology: Advances and Applications, Vol Volume 9, Pp 119-124 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	ciprofloxacin MIC vorinostat antimicrobial susceptibility Therapeutics. Pharmacology RM1-950
spellingShingle	ciprofloxacin MIC vorinostat antimicrobial susceptibility Therapeutics. Pharmacology RM1-950 Masadeh MM Alzoubi KH Al-azzam SI Al-buhairan AM Possible involvement of ROS generation in vorinostat pretreatment induced enhancement of the antibacterial activity of ciprofloxacin
description	Majed M Masadeh,1 Karem H Alzoubi,2 Sayer I Al-azzam,2 Ahlam M Al-buhairan3 1Department of Pharmaceutical Technology, 2Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid, Jordan; 3Department of Clinical Laboratory Sciences, King Saud University, Riyadh, Saudi Arabia Abstract: The mechanism underlying ciprofloxacin action involves interference with transcription and replication of bacterial DNA and, thus, the induction of double-strand breaks in DNA. It also involves elevated oxidative stress, which might contribute to bacterial cell death. Vorinostat was shown to induce oxidative DNA damage. The current work investigated a possible interactive effect of vorinostat on ciprofloxacin-induced cytotoxicity against a number of reference bacteria. Standard bacterial strains were Escherichia coli ATCC 35218, Staphylococcus aureus ATCC29213, Pseudomonas aeruginosa ATCC 9027, Staphylococcus epidermidis ATCC 12228, Acinetobacter baumannii ATCC 17978, Proteus mirabilis ATCC 12459, Klebsiella pneumoniae ATCC 13883, methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43300), and Streptococcus pneumoniae (ATCC 25923). The antibacterial activity of ciprofloxacin, with or without pretreatment of bacterial cells by vorinostat, was examined using the disc diffusion procedure and determination of the minimum inhibitory concentration (MIC) and zones of inhibition of bacterial growth. All tested bacterial strains showed sensitivity to ciprofloxacin. When pretreated with vorinostat, significantly larger zones of inhibition and smaller MIC values were observed in all bacterial strains compared to those treated with ciprofloxacin alone. In correlation, generation of reactive oxygen species (ROS) induced by the antibacterial action of ciprofloxacin was enhanced by treatment of bacterial cells with vorinostat. Results showed the possible agonistic properties of vorinostat when used together with ciprofloxacin. This could be related to the ability of these agents to enhance oxidative stress in bacterial cells. Keywords: flouroquinolones, MIC, histone deacetylase inhibitor, oxidative stress, antimicrobial susceptibility
format	article
author	Masadeh MM Alzoubi KH Al-azzam SI Al-buhairan AM
author_facet	Masadeh MM Alzoubi KH Al-azzam SI Al-buhairan AM
author_sort	Masadeh MM
title	Possible involvement of ROS generation in vorinostat pretreatment induced enhancement of the antibacterial activity of ciprofloxacin
title_short	Possible involvement of ROS generation in vorinostat pretreatment induced enhancement of the antibacterial activity of ciprofloxacin
title_full	Possible involvement of ROS generation in vorinostat pretreatment induced enhancement of the antibacterial activity of ciprofloxacin
title_fullStr	Possible involvement of ROS generation in vorinostat pretreatment induced enhancement of the antibacterial activity of ciprofloxacin
title_full_unstemmed	Possible involvement of ROS generation in vorinostat pretreatment induced enhancement of the antibacterial activity of ciprofloxacin
title_sort	possible involvement of ros generation in vorinostat pretreatment induced enhancement of the antibacterial activity of ciprofloxacin
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/7f02025171eb4abfaae32ac8f118642a
work_keys_str_mv	AT masadehmm possibleinvolvementofrosgenerationinvorinostatpretreatmentinducedenhancementoftheantibacterialactivityofciprofloxacin AT alzoubikh possibleinvolvementofrosgenerationinvorinostatpretreatmentinducedenhancementoftheantibacterialactivityofciprofloxacin AT alazzamsi possibleinvolvementofrosgenerationinvorinostatpretreatmentinducedenhancementoftheantibacterialactivityofciprofloxacin AT albuhairanam possibleinvolvementofrosgenerationinvorinostatpretreatmentinducedenhancementoftheantibacterialactivityofciprofloxacin
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Possible involvement of ROS generation in vorinostat pretreatment induced enhancement of the antibacterial activity of ciprofloxacin

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