Novel Type III Effectors in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

ABSTRACT Pseudomonas aeruginosa is a Gram-negative, opportunistic pathogen that causes chronic and acute infections in immunocompromised patients. Most P. aeruginosa strains encode an active type III secretion system (T3SS), utilized by the bacteria to deliver effector proteins from the bacterial ce...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	David Burstein, Shirley Satanower, Michal Simovitch, Yana Belnik, Meital Zehavi, Gal Yerushalmi, Shay Ben-Aroya, Tal Pupko, Ehud Banin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7f02ec7e262e4739a86c58fe62647c26
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7f02ec7e262e4739a86c58fe62647c26
record_format	dspace
spelling	oai:doaj.org-article:7f02ec7e262e4739a86c58fe62647c262021-11-15T15:41:34ZNovel Type III Effectors in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>10.1128/mBio.00161-152150-7511https://doaj.org/article/7f02ec7e262e4739a86c58fe62647c262015-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00161-15https://doaj.org/toc/2150-7511ABSTRACT Pseudomonas aeruginosa is a Gram-negative, opportunistic pathogen that causes chronic and acute infections in immunocompromised patients. Most P. aeruginosa strains encode an active type III secretion system (T3SS), utilized by the bacteria to deliver effector proteins from the bacterial cell directly into the cytoplasm of the host cell. Four T3SS effectors have been discovered and extensively studied in P. aeruginosa: ExoT, ExoS, ExoU, and ExoY. This is especially intriguing in light of P. aeruginosa's ability to infect a wide range of hosts. We therefore hypothesized that additional T3SS effectors that have not yet been discovered are encoded in the genome of P. aeruginosa. Here, we applied a machine learning classification algorithm to identify novel P. aeruginosa effectors. In this approach, various types of data are integrated to differentiate effectors from the rest of the open reading frames of the bacterial genome. Due to the lack of a sufficient learning set of positive effectors, our machine learning algorithm integrated genomic information from another Pseudomonas species and utilized dozens of features accounting for various aspects of the effector coding genes and their products. Twelve top-ranking predictions were experimentally tested for T3SS-specific translocation, leading to the discovery of two novel T3SS effectors. We demonstrate that these effectors are not part of the injection structural complex and report initial efforts toward their characterization. IMPORTANCE Pseudomonas aeruginosa uses a type III secretion system (T3SS) to secrete toxic proteins, termed effectors, directly into the cytoplasm of the host cell. The activation of this secretion system is correlated with disease severity and patient death. Compared with many other T3SS-utilizing pathogenic bacteria, P. aeruginosa has a fairly limited arsenal of effectors that have been identified. This is in sharp contrast with the wide range of hosts that this bacterium can infect. The discovery of two novel effectors described here is an important step toward better understanding of the virulence and host evasion mechanisms adopted by this versatile pathogen and may provide novel approaches to treat P. aeruginosa infections.David BursteinShirley SatanowerMichal SimovitchYana BelnikMeital ZehaviGal YerushalmiShay Ben-AroyaTal PupkoEhud BaninAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 2 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 David Burstein Shirley Satanower Michal Simovitch Yana Belnik Meital Zehavi Gal Yerushalmi Shay Ben-Aroya Tal Pupko Ehud Banin Novel Type III Effectors in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
description	ABSTRACT Pseudomonas aeruginosa is a Gram-negative, opportunistic pathogen that causes chronic and acute infections in immunocompromised patients. Most P. aeruginosa strains encode an active type III secretion system (T3SS), utilized by the bacteria to deliver effector proteins from the bacterial cell directly into the cytoplasm of the host cell. Four T3SS effectors have been discovered and extensively studied in P. aeruginosa: ExoT, ExoS, ExoU, and ExoY. This is especially intriguing in light of P. aeruginosa's ability to infect a wide range of hosts. We therefore hypothesized that additional T3SS effectors that have not yet been discovered are encoded in the genome of P. aeruginosa. Here, we applied a machine learning classification algorithm to identify novel P. aeruginosa effectors. In this approach, various types of data are integrated to differentiate effectors from the rest of the open reading frames of the bacterial genome. Due to the lack of a sufficient learning set of positive effectors, our machine learning algorithm integrated genomic information from another Pseudomonas species and utilized dozens of features accounting for various aspects of the effector coding genes and their products. Twelve top-ranking predictions were experimentally tested for T3SS-specific translocation, leading to the discovery of two novel T3SS effectors. We demonstrate that these effectors are not part of the injection structural complex and report initial efforts toward their characterization. IMPORTANCE Pseudomonas aeruginosa uses a type III secretion system (T3SS) to secrete toxic proteins, termed effectors, directly into the cytoplasm of the host cell. The activation of this secretion system is correlated with disease severity and patient death. Compared with many other T3SS-utilizing pathogenic bacteria, P. aeruginosa has a fairly limited arsenal of effectors that have been identified. This is in sharp contrast with the wide range of hosts that this bacterium can infect. The discovery of two novel effectors described here is an important step toward better understanding of the virulence and host evasion mechanisms adopted by this versatile pathogen and may provide novel approaches to treat P. aeruginosa infections.
format	article
author	David Burstein Shirley Satanower Michal Simovitch Yana Belnik Meital Zehavi Gal Yerushalmi Shay Ben-Aroya Tal Pupko Ehud Banin
author_facet	David Burstein Shirley Satanower Michal Simovitch Yana Belnik Meital Zehavi Gal Yerushalmi Shay Ben-Aroya Tal Pupko Ehud Banin
author_sort	David Burstein
title	Novel Type III Effectors in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_short	Novel Type III Effectors in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full	Novel Type III Effectors in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_fullStr	Novel Type III Effectors in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full_unstemmed	Novel Type III Effectors in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_sort	novel type iii effectors in <named-content content-type="genus-species">pseudomonas aeruginosa</named-content>
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/7f02ec7e262e4739a86c58fe62647c26
work_keys_str_mv	AT davidburstein noveltypeiiieffectorsinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent AT shirleysatanower noveltypeiiieffectorsinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent AT michalsimovitch noveltypeiiieffectorsinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent AT yanabelnik noveltypeiiieffectorsinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent AT meitalzehavi noveltypeiiieffectorsinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent AT galyerushalmi noveltypeiiieffectorsinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent AT shaybenaroya noveltypeiiieffectorsinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent AT talpupko noveltypeiiieffectorsinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent AT ehudbanin noveltypeiiieffectorsinnamedcontentcontenttypegenusspeciespseudomonasaeruginosanamedcontent
_version_	1718427650034237440

Novel Type III Effectors in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

Ejemplares similares