Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model

Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model

Muthu Irulappan Sriram, Selvaraj Barath Mani Kanth, Kalimuthu Kalishwaralal, Sangiliyandi GurunathanDepartment of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University, Tamilnadu, IndiaAbstract: Nanomedicine concerns the use of precision-engineered nanomaterials to devel...

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Autores principales: Muthu Irulappan Sriram, Selvaraj Barath Mani Kanth, Kalimuthu Kalishwaralal, et al
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2010
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/7f06c97c7ab1435589e0e5d61aa42547
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spelling oai:doaj.org-article:7f06c97c7ab1435589e0e5d61aa425472021-12-02T01:42:41ZAntitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model1176-91141178-2013https://doaj.org/article/7f06c97c7ab1435589e0e5d61aa425472010-09-01T00:00:00Zhttp://www.dovepress.com/antitumor-activity-of-silver-nanoparticles-in-daltonrsquos-lymphoma-as-a5368https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Muthu Irulappan Sriram, Selvaraj Barath Mani Kanth, Kalimuthu Kalishwaralal, Sangiliyandi GurunathanDepartment of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University, Tamilnadu, IndiaAbstract: Nanomedicine concerns the use of precision-engineered nanomaterials to develop novel therapeutic and diagnostic modalities for human use. The present study demonstrates the efficacy of biologically synthesized silver nanoparticles (AgNPs) as an antitumor agent using Dalton’s lymphoma ascites (DLA) cell lines in vitro and in vivo. The AgNPs showed dose-dependent cytotoxicity against DLA cells through activation of the caspase 3 enzyme, leading to induction of apoptosis which was further confirmed through resulting nuclear fragmentation. Acute toxicity, ie, convulsions, hyperactivity and chronic toxicity such as increased body weight and abnormal hematologic parameters did not occur. AgNPs significantly increased the survival time in the tumor mouse model by about 50% in comparison with tumor controls. AgNPs also decreased the volume of ascitic fluid in tumor-bearing mice by 65%, thereby returning body weight to normal. Elevated white blood cell and platelet counts in ascitic fluid from the tumor-bearing mice were brought to near-normal range. Histopathologic analysis of ascitic fluid showed a reduction in DLA cell count in tumor-bearing mice treated with AgNPs. These findings confirm the antitumor properties of AgNPs, and suggest that they may be a cost-effective alternative in the treatment of cancer and angiogenesis-related disorders.Keywords: antitumor, silver nanoparticles, Dalton’s lymphoma, ascites Muthu Irulappan SriramSelvaraj Barath Mani KanthKalimuthu Kalishwaralalet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 753-762 (2010)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Muthu Irulappan Sriram
Selvaraj Barath Mani Kanth
Kalimuthu Kalishwaralal
et al
Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model
description Muthu Irulappan Sriram, Selvaraj Barath Mani Kanth, Kalimuthu Kalishwaralal, Sangiliyandi GurunathanDepartment of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University, Tamilnadu, IndiaAbstract: Nanomedicine concerns the use of precision-engineered nanomaterials to develop novel therapeutic and diagnostic modalities for human use. The present study demonstrates the efficacy of biologically synthesized silver nanoparticles (AgNPs) as an antitumor agent using Dalton’s lymphoma ascites (DLA) cell lines in vitro and in vivo. The AgNPs showed dose-dependent cytotoxicity against DLA cells through activation of the caspase 3 enzyme, leading to induction of apoptosis which was further confirmed through resulting nuclear fragmentation. Acute toxicity, ie, convulsions, hyperactivity and chronic toxicity such as increased body weight and abnormal hematologic parameters did not occur. AgNPs significantly increased the survival time in the tumor mouse model by about 50% in comparison with tumor controls. AgNPs also decreased the volume of ascitic fluid in tumor-bearing mice by 65%, thereby returning body weight to normal. Elevated white blood cell and platelet counts in ascitic fluid from the tumor-bearing mice were brought to near-normal range. Histopathologic analysis of ascitic fluid showed a reduction in DLA cell count in tumor-bearing mice treated with AgNPs. These findings confirm the antitumor properties of AgNPs, and suggest that they may be a cost-effective alternative in the treatment of cancer and angiogenesis-related disorders.Keywords: antitumor, silver nanoparticles, Dalton’s lymphoma, ascites
format article
author Muthu Irulappan Sriram
Selvaraj Barath Mani Kanth
Kalimuthu Kalishwaralal
et al
author_facet Muthu Irulappan Sriram
Selvaraj Barath Mani Kanth
Kalimuthu Kalishwaralal
et al
author_sort Muthu Irulappan Sriram
title Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model
title_short Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model
title_full Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model
title_fullStr Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model
title_full_unstemmed Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model
title_sort antitumor activity of silver nanoparticles in dalton’s lymphoma ascites tumor model
publisher Dove Medical Press
publishDate 2010
url https://doaj.org/article/7f06c97c7ab1435589e0e5d61aa42547
work_keys_str_mv AT muthuirulappansriram antitumoractivityofsilvernanoparticlesindaltonamprsquoslymphomaascitestumormodel
AT selvarajbarathmanikanth antitumoractivityofsilvernanoparticlesindaltonamprsquoslymphomaascitestumormodel
AT kalimuthukalishwaralal antitumoractivityofsilvernanoparticlesindaltonamprsquoslymphomaascitestumormodel
AT etal antitumoractivityofsilvernanoparticlesindaltonamprsquoslymphomaascitestumormodel
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