Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.

Many Penicillium species could produce extracellular enzyme systems with good lignocellulose hydrolysis performance. However, these species and their enzyme systems are still poorly understood and explored due to the lacking of genetic information. Here, we present the genomic and secretomic analyse...

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Autores principales: Guodong Liu, Lei Zhang, Xiaomin Wei, Gen Zou, Yuqi Qin, Liang Ma, Jie Li, Huajun Zheng, Shengyue Wang, Chengshu Wang, Luying Xun, Guo-Ping Zhao, Zhihua Zhou, Yinbo Qu
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Publicado: Public Library of Science (PLoS) 2013
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Acceso en línea:https://doaj.org/article/7f098f6063a840c0a7f2018783814f51
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spelling oai:doaj.org-article:7f098f6063a840c0a7f2018783814f512021-11-18T07:59:00ZGenomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.1932-620310.1371/journal.pone.0055185https://doaj.org/article/7f098f6063a840c0a7f2018783814f512013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23383313/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Many Penicillium species could produce extracellular enzyme systems with good lignocellulose hydrolysis performance. However, these species and their enzyme systems are still poorly understood and explored due to the lacking of genetic information. Here, we present the genomic and secretomic analyses of Penicillium decumbens that has been used in industrial production of lignocellulolytic enzymes in China for more than fifteen years. Comparative genomics analysis with the phylogenetically most similar species Penicillium chrysogenum revealed that P. decumbens has evolved with more genes involved in plant cell wall degradation, but fewer genes in cellular metabolism and regulation. Compared with the widely used cellulase producer Trichoderma reesei, P. decumbens has a lignocellulolytic enzyme system with more diverse components, particularly for cellulose binding domain-containing proteins and hemicellulases. Further, proteomic analysis of secretomes revealed that P. decumbens produced significantly more lignocellulolytic enzymes in the medium with cellulose-wheat bran as the carbon source than with glucose. The results expand our knowledge on the genetic information of lignocellulolytic enzyme systems in Penicillium species, and will facilitate rational strain improvement for the production of highly efficient enzyme systems used in lignocellulose utilization from Penicillium species.Guodong LiuLei ZhangXiaomin WeiGen ZouYuqi QinLiang MaJie LiHuajun ZhengShengyue WangChengshu WangLuying XunGuo-Ping ZhaoZhihua ZhouYinbo QuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e55185 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Guodong Liu
Lei Zhang
Xiaomin Wei
Gen Zou
Yuqi Qin
Liang Ma
Jie Li
Huajun Zheng
Shengyue Wang
Chengshu Wang
Luying Xun
Guo-Ping Zhao
Zhihua Zhou
Yinbo Qu
Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.
description Many Penicillium species could produce extracellular enzyme systems with good lignocellulose hydrolysis performance. However, these species and their enzyme systems are still poorly understood and explored due to the lacking of genetic information. Here, we present the genomic and secretomic analyses of Penicillium decumbens that has been used in industrial production of lignocellulolytic enzymes in China for more than fifteen years. Comparative genomics analysis with the phylogenetically most similar species Penicillium chrysogenum revealed that P. decumbens has evolved with more genes involved in plant cell wall degradation, but fewer genes in cellular metabolism and regulation. Compared with the widely used cellulase producer Trichoderma reesei, P. decumbens has a lignocellulolytic enzyme system with more diverse components, particularly for cellulose binding domain-containing proteins and hemicellulases. Further, proteomic analysis of secretomes revealed that P. decumbens produced significantly more lignocellulolytic enzymes in the medium with cellulose-wheat bran as the carbon source than with glucose. The results expand our knowledge on the genetic information of lignocellulolytic enzyme systems in Penicillium species, and will facilitate rational strain improvement for the production of highly efficient enzyme systems used in lignocellulose utilization from Penicillium species.
format article
author Guodong Liu
Lei Zhang
Xiaomin Wei
Gen Zou
Yuqi Qin
Liang Ma
Jie Li
Huajun Zheng
Shengyue Wang
Chengshu Wang
Luying Xun
Guo-Ping Zhao
Zhihua Zhou
Yinbo Qu
author_facet Guodong Liu
Lei Zhang
Xiaomin Wei
Gen Zou
Yuqi Qin
Liang Ma
Jie Li
Huajun Zheng
Shengyue Wang
Chengshu Wang
Luying Xun
Guo-Ping Zhao
Zhihua Zhou
Yinbo Qu
author_sort Guodong Liu
title Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.
title_short Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.
title_full Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.
title_fullStr Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.
title_full_unstemmed Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.
title_sort genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of penicillium decumbens.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/7f098f6063a840c0a7f2018783814f51
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