Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases

Group B <i>Streptococcus</i> (GBS) is an important pathogen of neonatal infections, and the clonal complex (CC)-17/serotype III GBS strain has emerged as the dominant strain. The clinical manifestations of CC17/III GBS sepsis may vary greatly but have not been well-investigated. A total...

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Autores principales:	Jen-Fu Hsu, Jang-Jih Lu, Chih Lin, Shih-Ming Chu, Lee-Chung Lin, Mei-Yin Lai, Hsuan-Rong Huang, Ming-Chou Chiang, Ming-Horng Tsai
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	group B <i>Streptococcus</i> CRISPR multilocus sequence typing antimicrobial resistance phage Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/7f0b4f77ba79480596f21f4377a55b19
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spelling	oai:doaj.org-article:7f0b4f77ba79480596f21f4377a55b192021-11-11T17:06:12ZClustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases10.3390/ijms2221116261422-00671661-6596https://doaj.org/article/7f0b4f77ba79480596f21f4377a55b192021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11626https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Group B <i>Streptococcus</i> (GBS) is an important pathogen of neonatal infections, and the clonal complex (CC)-17/serotype III GBS strain has emerged as the dominant strain. The clinical manifestations of CC17/III GBS sepsis may vary greatly but have not been well-investigated. A total of 103 CC17/III GBS isolates that caused neonatal invasive diseases were studied using a new approach based on clustered regularly interspaced short palindromic repeats (CRISPR) loci and restriction fragment length polymorphism (RFLP) analyses. All spacers of CRISPR loci were sequenced and analyzed with the clinical presentations. After CRISPR-RFLP analyses, a total of 11 different patterns were observed among the 103 CRISPR-positive GBS isolates. GBS isolates with the same RFLP patterns were found to have highly comparable spacer contents. Comparative sequence analysis of the CRISPR1 spacer content revealed that it is highly diverse and consistent with the dynamics of this system. A total of 29 of 43 (67.4%) spacers displayed homology to reported phage and plasmid DNA sequences. In addition, all CC17/III GBS isolates could be categorized into three subgroups based on the CRISPR-RFLP patterns and eBURST analysis. The CC17/III GBS isolates with a specific CRISPR-RFLP pattern were more significantly associated with occurrences of severe sepsis (57.1% vs. 29.3%, <i>p</i> = 0.012) and meningitis (50.0% vs. 20.8%, <i>p</i> = 0.009) than GBS isolates with RFLP lengths between 1000 and 1300 bp. Whole-genome sequencing was also performed to verify the differences between CC17/III GBS isolates with different CRISPR-RFLP patterns. We concluded that the CRISPR-RFLP analysis is potentially applicable to categorizing CC17/III GBS isolates, and a specific CRISPR-RFLP pattern could be used as a new biomarker to predict meningitis and illness severity after further verification.Jen-Fu HsuJang-Jih LuChih LinShih-Ming ChuLee-Chung LinMei-Yin LaiHsuan-Rong HuangMing-Chou ChiangMing-Horng TsaiMDPI AGarticlegroup B <i>Streptococcus</i>CRISPRmultilocus sequence typingantimicrobial resistancephageBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11626, p 11626 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	group B <i>Streptococcus</i> CRISPR multilocus sequence typing antimicrobial resistance phage Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	group B <i>Streptococcus</i> CRISPR multilocus sequence typing antimicrobial resistance phage Biology (General) QH301-705.5 Chemistry QD1-999 Jen-Fu Hsu Jang-Jih Lu Chih Lin Shih-Ming Chu Lee-Chung Lin Mei-Yin Lai Hsuan-Rong Huang Ming-Chou Chiang Ming-Horng Tsai Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases
description	Group B <i>Streptococcus</i> (GBS) is an important pathogen of neonatal infections, and the clonal complex (CC)-17/serotype III GBS strain has emerged as the dominant strain. The clinical manifestations of CC17/III GBS sepsis may vary greatly but have not been well-investigated. A total of 103 CC17/III GBS isolates that caused neonatal invasive diseases were studied using a new approach based on clustered regularly interspaced short palindromic repeats (CRISPR) loci and restriction fragment length polymorphism (RFLP) analyses. All spacers of CRISPR loci were sequenced and analyzed with the clinical presentations. After CRISPR-RFLP analyses, a total of 11 different patterns were observed among the 103 CRISPR-positive GBS isolates. GBS isolates with the same RFLP patterns were found to have highly comparable spacer contents. Comparative sequence analysis of the CRISPR1 spacer content revealed that it is highly diverse and consistent with the dynamics of this system. A total of 29 of 43 (67.4%) spacers displayed homology to reported phage and plasmid DNA sequences. In addition, all CC17/III GBS isolates could be categorized into three subgroups based on the CRISPR-RFLP patterns and eBURST analysis. The CC17/III GBS isolates with a specific CRISPR-RFLP pattern were more significantly associated with occurrences of severe sepsis (57.1% vs. 29.3%, <i>p</i> = 0.012) and meningitis (50.0% vs. 20.8%, <i>p</i> = 0.009) than GBS isolates with RFLP lengths between 1000 and 1300 bp. Whole-genome sequencing was also performed to verify the differences between CC17/III GBS isolates with different CRISPR-RFLP patterns. We concluded that the CRISPR-RFLP analysis is potentially applicable to categorizing CC17/III GBS isolates, and a specific CRISPR-RFLP pattern could be used as a new biomarker to predict meningitis and illness severity after further verification.
format	article
author	Jen-Fu Hsu Jang-Jih Lu Chih Lin Shih-Ming Chu Lee-Chung Lin Mei-Yin Lai Hsuan-Rong Huang Ming-Chou Chiang Ming-Horng Tsai
author_facet	Jen-Fu Hsu Jang-Jih Lu Chih Lin Shih-Ming Chu Lee-Chung Lin Mei-Yin Lai Hsuan-Rong Huang Ming-Chou Chiang Ming-Horng Tsai
author_sort	Jen-Fu Hsu
title	Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases
title_short	Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases
title_full	Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases
title_fullStr	Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases
title_full_unstemmed	Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases
title_sort	clustered regularly interspaced short palindromic repeat analysis of clonal complex 17 serotype iii group b <i>streptococcus</i> strains causing neonatal invasive diseases
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7f0b4f77ba79480596f21f4377a55b19
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Clustered Regularly Interspaced Short Palindromic Repeat Analysis of Clonal Complex 17 Serotype III Group B <i>Streptococcus</i> Strains Causing Neonatal Invasive Diseases

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