A novel laser-Doppler flowmetry assisted murine model of acute hindlimb ischemia-reperfusion for free flap research.

Suitable and reproducible experimental models of translational research in reconstructive surgery that allow in-vivo investigation of diverse molecular and cellular mechanisms are still limited. To this end we created a novel murine model of acute hindlimb ischemia-reperfusion to mimic a microsurgic...

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Autores principales:	Tolga Taha Sönmez, Othman Al-Sawaf, Gerald Brandacher, Isabella Kanzler, Nancy Tuchscheerer, Mersedeh Tohidnezhad, Anastasios Kanatas, Matthias Knobe, Athanassios Fragoulis, René Tolba, David Mitchell, Thomas Pufe, Christoph Jan Wruck, Frank Hölzle, Elisa Anamaria Liehn
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f0c4fcf399740389774704aec555183
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spelling	oai:doaj.org-article:7f0c4fcf399740389774704aec5551832021-11-18T07:40:47ZA novel laser-Doppler flowmetry assisted murine model of acute hindlimb ischemia-reperfusion for free flap research.1932-620310.1371/journal.pone.0066498https://doaj.org/article/7f0c4fcf399740389774704aec5551832013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23840492/?tool=EBIhttps://doaj.org/toc/1932-6203Suitable and reproducible experimental models of translational research in reconstructive surgery that allow in-vivo investigation of diverse molecular and cellular mechanisms are still limited. To this end we created a novel murine model of acute hindlimb ischemia-reperfusion to mimic a microsurgical free flap procedure. Thirty-six C57BL6 mice (n = 6/group) were assigned to one control and five experimental groups (subject to 6, 12, 96, 120 hours and 14 days of reperfusion, respectively) following 4 hours of complete hindlimb ischemia. Ischemia and reperfusion were monitored using Laser-Doppler Flowmetry. Hindlimb tissue components (skin and muscle) were investigated using histopathology, quantitative immunohistochemistry and immunofluorescence. Despite massive initial tissue damage induced by ischemia-reperfusion injury, the structure of the skin component was restored after 96 hours. During the same time, muscle cells were replaced by young myotubes. In addition, initial neuromuscular dysfunction, edema and swelling resolved by day 4. After two weeks, no functional or neuromuscular deficits were detectable. Furthermore, upregulation of VEGF and tissue infiltration with CD34-positive stem cells led to new capillary formation, which peaked with significantly higher values after two weeks. These data indicate that our model is suitable to investigate cellular and molecular tissue alterations from ischemia-reperfusion such as occur during free flap procedures.Tolga Taha SönmezOthman Al-SawafGerald BrandacherIsabella KanzlerNancy TuchscheererMersedeh TohidnezhadAnastasios KanatasMatthias KnobeAthanassios FragoulisRené TolbaDavid MitchellThomas PufeChristoph Jan WruckFrank HölzleElisa Anamaria LiehnPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e66498 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tolga Taha Sönmez Othman Al-Sawaf Gerald Brandacher Isabella Kanzler Nancy Tuchscheerer Mersedeh Tohidnezhad Anastasios Kanatas Matthias Knobe Athanassios Fragoulis René Tolba David Mitchell Thomas Pufe Christoph Jan Wruck Frank Hölzle Elisa Anamaria Liehn A novel laser-Doppler flowmetry assisted murine model of acute hindlimb ischemia-reperfusion for free flap research.
description	Suitable and reproducible experimental models of translational research in reconstructive surgery that allow in-vivo investigation of diverse molecular and cellular mechanisms are still limited. To this end we created a novel murine model of acute hindlimb ischemia-reperfusion to mimic a microsurgical free flap procedure. Thirty-six C57BL6 mice (n = 6/group) were assigned to one control and five experimental groups (subject to 6, 12, 96, 120 hours and 14 days of reperfusion, respectively) following 4 hours of complete hindlimb ischemia. Ischemia and reperfusion were monitored using Laser-Doppler Flowmetry. Hindlimb tissue components (skin and muscle) were investigated using histopathology, quantitative immunohistochemistry and immunofluorescence. Despite massive initial tissue damage induced by ischemia-reperfusion injury, the structure of the skin component was restored after 96 hours. During the same time, muscle cells were replaced by young myotubes. In addition, initial neuromuscular dysfunction, edema and swelling resolved by day 4. After two weeks, no functional or neuromuscular deficits were detectable. Furthermore, upregulation of VEGF and tissue infiltration with CD34-positive stem cells led to new capillary formation, which peaked with significantly higher values after two weeks. These data indicate that our model is suitable to investigate cellular and molecular tissue alterations from ischemia-reperfusion such as occur during free flap procedures.
format	article
author	Tolga Taha Sönmez Othman Al-Sawaf Gerald Brandacher Isabella Kanzler Nancy Tuchscheerer Mersedeh Tohidnezhad Anastasios Kanatas Matthias Knobe Athanassios Fragoulis René Tolba David Mitchell Thomas Pufe Christoph Jan Wruck Frank Hölzle Elisa Anamaria Liehn
author_facet	Tolga Taha Sönmez Othman Al-Sawaf Gerald Brandacher Isabella Kanzler Nancy Tuchscheerer Mersedeh Tohidnezhad Anastasios Kanatas Matthias Knobe Athanassios Fragoulis René Tolba David Mitchell Thomas Pufe Christoph Jan Wruck Frank Hölzle Elisa Anamaria Liehn
author_sort	Tolga Taha Sönmez
title	A novel laser-Doppler flowmetry assisted murine model of acute hindlimb ischemia-reperfusion for free flap research.
title_short	A novel laser-Doppler flowmetry assisted murine model of acute hindlimb ischemia-reperfusion for free flap research.
title_full	A novel laser-Doppler flowmetry assisted murine model of acute hindlimb ischemia-reperfusion for free flap research.
title_fullStr	A novel laser-Doppler flowmetry assisted murine model of acute hindlimb ischemia-reperfusion for free flap research.
title_full_unstemmed	A novel laser-Doppler flowmetry assisted murine model of acute hindlimb ischemia-reperfusion for free flap research.
title_sort	novel laser-doppler flowmetry assisted murine model of acute hindlimb ischemia-reperfusion for free flap research.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7f0c4fcf399740389774704aec555183
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A novel laser-Doppler flowmetry assisted murine model of acute hindlimb ischemia-reperfusion for free flap research.

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